A comparative study on the effects of pristine and functionalized single-walled carbon nanotubes on osteoblasts: ultrastructural and biochemical properties

A comparative study was performed to investigate the ultrastructural and biomolecular properties of osteoblasts induced by three types of single-walled carbon nanotubes (SWNTs). The results on cellular uptake and ultrastructural alteration indicate that SWNTs enter osteoblasts by endocytosis. SWNTs-COOH and SWNTs-OH particles were freely dispersed in the cytoplasm, while pristine SWNTs were localized to the periphery of the cell. Both SWNTs-OH and SWNTs-COOH promoted cell changes in cell activity regarding mRNA expression at doses of 50 and 100 μg/mL in the first 24 h. When treated with 50 μg/mL SWNTs-COOH for 48 h, the expression of type I collagen increased by 6.3-fold (for MG63) or 9.1-fold (for primary osteoblasts) compared with the control group. The present study observed for the first time that SWNTs-COOH initiated the prompt and the maximum upregulation of type I collagen gene expression, and simultaneously induced the expansion of the endoplasmic reticulum for increased protein synthesis, which in turn accelerated the mineralization process. However, impaired cell properties and mitochondrial injury were detected following treatment with SWNTs at 100 μg/mL after 48 h. In conclusion, we believe that SWNTs-COOH is a good candidate for the fabrication of biomedical scaffolds for bone regeneration.     

螺旋管中间介质气化器可行性研究

A novel intermediate fluid vaporization (IFV) technology for LNG re-gasification process with spiral-wound heat exchanging tubes is proposed. The new IFV project combines the advantage of running the shell and tube heat exchangers at high pressure with the advantage of compact space of heat exchangers. Thermal analysis on the two processes of forced convection and vaporization type heat transfer in the spiral wound tubes and vapor condensation /re-boiling type of heat transfer via intermediate fluid in shell side shows the feasibility of this promising technology.     

血液中佐匹克隆全自动固相萃取高效液相色谱质谱检测方法研究

目的:建立血液中佐匹克隆全自动固相萃取高效液相色谱-三重四级杆串联质谱(LC/MS/MS)检测方法。方法:采用全自动固相萃取进行提取,用Oasis HLB固相萃取柱,使用二氯甲烷洗脱。洗脱物浓缩后用Eclipse Plus C18柱(3.5μm×2.1×100mm)分离,LC/MS/MS分析。结果:采用本文方法检测血液中佐匹克隆的检出限为0.1ng/ml,回收率大于90.0%。结论:本方法具有操作简单、灵敏度高和重现性好等优点,可应用于血液中佐匹克隆的检验鉴定。     

基于人机工程的汽车组合仪表设计研究

重点介绍了轿车内部组合仪表的布置及校核方法。通过以人机工程为依据,对组合仪表的位置、视野盲区、反光、眩目进行理论分析,同时结合工作经验,提出了一些设计经验,对新产品的设计开发具有指导意义。     

界面层剪切试验方法综述

采用界面剪切试验方法对不同材料组成的复合结构层间抗剪性能进行了研究,通过对比分析不同情况下的界面层剪切试验方法,探讨了其常用试验方法的使用情况及优缺点,并提出采用局部脱空叠层梁模型研究的试验方法,为界面层非线性粘结滑移状态的剪切特性试验研究提供参考。     

Fractionation and characterization of ɛ-poly-l-lysine from Streptomyces albulus CGMCC 1986

ɛ-Poly-l-lysine (ɛ-PLL) produced by Streptomyces albulus CGMCC 1986 was fractionated using ultra-filtration technique with 2 and 5 kDa cut-offs of membrane. The number-average molecular weight of each fraction was determined by ¹H nuclear magnetic resonance (NMR) method. The number-average molecular weights of the cutoffs of 5 and 2 kDa and the filtrate are 4,230.95, 3,687.80, and 1,900.82 Da, respectively. ¹H NMR indicates the chemical shifts of α-H, β-H, γ-H, δ-H, and ɛ-H are very similar to all the fractions. Fourier transform-infrared (FTIR) spectra showed that the ɛ-PLL solid samples obtained by freeze-drying at pH 5 with molecular weights higher than 2 kDa take on a β-turn conformation, however, the fraction with molecular weight smaller than 2 kDa adopts random coil structure. The antibacterial test proved that the fraction between 2 and 5 kDa of membranes behaves the highest antibacterial activity than other fractions for the test strains of Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Escherichia coli, and Shigella.     

Antiproliferative and antioxidant properties of anthocyanin-rich extract from açai

An anthocyanin-rich extract, generated from açai (AEA), was investigated for its antioxidant properties and antiproliferative activity against C-6 rat brain glioma cells and MDA-468 human breast cancer cells. AEA has an ORAC value of 2589 μmoles trolox equivalents (TE)/g dried powder and a DPPH radical-scavenging activity of 1208 μmoles TE/g, suggesting that AEA is an exceptional source of natural antioxidants. In addition, AEA remarkably suppresses proliferation of C-6 rat brain glioma cells, but has no effect on the growth of MDA-468 human breast cancer cells. Further experiments demonstrated that the AEA treatment dose-dependently inhibited the growth of C-6 rat glioma cells with an IC₅₀ of 121 μg/ml. The DNA ladder fragmentation results indicated that AEA induced apoptosis of C-6 rat brain glioma cells. To compare açai with other anthocyanin-rich extracts, a number of berry extracts, including blueberry, strawberry, raspberry, blackberry and wolfberry, were assessed for potential antiproliferative activity against C-6 rat brain glioma cells. However, none of them showed suppressing effect. The results suggest that the active antiproliferative constituents in AEA are unlikely to be anthocyanins normally found in common berries.     

高温超导量子干涉磁强计的发展现状及其应用

本文介绍了高温超导量子干涉磁强计的发展现状及其在生物磁测量、地磁测量、无损探伤及扫描SQUID显微镜等方面的应用。     

昆明新机场航站楼钢彩带贝雷架支撑系统设计与应用

昆明新机场航站楼钢彩带为双层群拱结构,施工时采用自行设计的贝雷架支撑系统,此系统主要由贝雷片和支撑架组合而成.根据群拱结构特点和吊装顺序,利用有限元软件SAP2000对吊装过程的胎架受力和变形进行了分析计算,计算结果表明自行设计的贝雷架支撑系统满足钢彩带施工要求.介绍了贝雷架的安装过程.采用全站仪对贝雷架卸载前后的变形进行了监测,发现卸载前后的胎架变形实测值与模拟值基本吻合.     

心脏病与高频心电研究

寻求心脏病的早期诊断方法是当务之急,高频心电除了具有较宽的通频带和较小信号幅度外,它比常规心电图具有更多的有关心脏活动状态的信息,可以观察到更多有关心脏主要部位病变的细节,能揭示出QRS综合波上某些部位的波形特征,所谓切迹(motch)和转折(slur)。本文重点研究高频心电的分析和识别方法及其技术实现。