Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity

For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function.     

Enhanced mechanical,thermal and dielectric properties of polyimide nanocomposites containing SiCp (SiCw) nanofillers for high energy-storage applications

Journal of Polymer Research - In this paper, we aimed to develop the nanocarrier based on poly (N-isopropyl acrylamide)—allyl acetoacetate grafted MoS2 nanosheets. The obtained polymer...     

Prediction of rock burst in underground caverns based on rough set and extensible comprehensive evaluation

Bulletin of Engineering Geology and the Environment - In high terrestrial stress regions, rock burst is a major geological disaster influencing underground engineering construction significantly....     

Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.     

职工食堂建筑设计的体验

伴随企业的不断发展,职工也不断增多,许多企业为员工建起了职工食堂。然而职工食堂设计施工并不是一项普通的工程,需要考虑的因素很多。本文就职工食堂设计要注意的事项进行了分析,浅谈了笔者对于职工食堂设计的体验。     

城市中心高架钢桥施工期保畅通实践

改造老旧道路、修建高架桥的施工期会加剧道路的拥堵。在郑州北三环快速化改造中,通过采取组织、管理、技术、经济等综合措施,实现了施工期原有主干道路畅通的目标,可为类似工程提供经验和借鉴。     

基于3DS MAX的铁路客运站旅客出站诱导设计模拟与评价

本文采用层次分析法建立了旅客诱导评价模型,通过模拟实验,分别对汉口火车站现状和设计方案进行了评价,为铁路客运站诱导设计方案评价提出了一种新的方法。