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The data of literature about the structure of cytoskeleton proteins in the normal and malignant cells are analyzed. The possibilities of using a specific polyclonal serum and a monoclonal antibody for a more precise ascertainment of the origin and diagnostic of tumours are discussed. The intermediate and active filaments may be used as a new class of histochemical markers. Laminin, the protein of basement membranes, probably, plays an important role in metastatic spreading.  相似文献   
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Summary The problem of fairly densily packed particles with rate independent though otherwise arbitrary interaction is investigated in two dimensions. It is shown that slow changes in a statistically uniform medium in the pre-failure deformation state can be described by a stiffness tensor. An approximate expression for this tensor is obtained from first principles. The connection with the perfectly random heterogeneous medium is established. A simple example for round rough particles in an isotropic packing of four contacts is given.With 8 Figure  相似文献   
225.
The usefulness of selected PCR-protocols for the detection of Salmonella in 117 samples of animal origin (17 raw minced meat, 27 raw chicken meat, 8 raw sausages, and 25 egg samples, as well as 18 poultry faecal, and caecal swabs samples) and DNA-fingerprinting typing is shown. To establish an accurate PCR-procedure for Salmonella detection the following parameters were evaluated: two pre-PCR concentration procedures, centrifugation and immunomagnetic separation (IMS) using Dynabeads anti- Salmonella; the specificity and sensitivity of 10 sets of primers; and different conditions of the amplification reaction. In light of the results obtained from the use of PCR-based procedures alone or in combination with conventional methods, the following findings can be underlined: First, IMS is more efficient than centrifugation in the recovery of Salmonella. Second, the selected IMS/PCR-detection protocol is less time-consuming (45 h) than the IMS/culture procedure (90 h), and a good concordance between them was found when the Kappa coefficient was calculated (0·87). Third, PCR-ribotyping technique showed a very low discrimination power, being able to differentiate only three profiles. Fourth, RAPD technique using specific primers supports previous works in which it was proposed as a simple and useful tool for discriminating isolates between and within serotypes. Fifth, The efficiency, rapidity, and flexibility of the PCR-protocols applied were high, and they can be performed using two PCR-programs and the same basic equipment.  相似文献   
226.
Results of a numerical investigation of heat and mass transfer at the initial stage of fire within a building in combustion of kerosene, carried out with the use of a three-dimensional mathematical field model, are presented. A comparison of the distribution of the averaged temperatures and velocities along the vertical axis of the convective column and of the mass rates of gas flows through an open opening with the experimental data has been made. Substantial three-dimensional inhomogeneities of the velocities and the temperatures in the near-ceiling layer have been revealed. It has been established that near the opening there is a critical separation zone which influences significantly the parameters of natural gas transfer.  相似文献   
227.
Coarse filters for shape matching   总被引:3,自引:0,他引:3  
This article describes the coarse shape filters that support the 3D, Internet-based search engine ShapeSifter, which aims to locate parts already in production that have a shape similar to a desired new part. The search engine analyzes the target model's shape characteristics and performs a similarity match on the database's contents. Our main challenge is identifying shape metrics that produce effective characterizations of 3D models for similarity comparison. We focus on using three novel convex hull-based indices to carry out a preliminary coarse filtering of candidates prior to more detailed analysis. We also describe the crucial role played by two databases of benchmark objects. Unlike other shape-matching systems, we chose ShapeSifter's architecture (multistep query), 3D representation (triangulated mesh), and implementation (Java3D) to allow deployment online once it matures  相似文献   
228.
This work is aimed at developing a predictive capability for the quantitative assessment of crack growth under fatigue loadings. The crack growth rate relation, ΔaΔN, may involve all three stress intensity factors k1-k3 such that the direction of crack growth may not be known in advance and must be predicted from a preassumed criterion. In principle, both the stress amplitude and the mean stress level should be included in the original expression for ΔaΔN.The strain energy density factor range, ΔS, is found to be a convenient parameter for predicting fatigue crack growth and can be applied expediently to examine the combined influence of crack geometry, complex loadings and material properties. Assumed is the accumulation of energy, ΔWΔV, stored in an element ahead of the crack which triggers subcritical crack growth upon reaching a number of loading cycle, say ΔN. The proposed δaΔN relationship includes both the stress amplitude and mean stress effects.  相似文献   
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Efficient data layout is an important aspect of the compilation process. A model for the creation of perfect memory maps for large-scale parallel machines capable of user-controlled partitionable single-instruction-multiple data/single-program-multiple data (SIMD/SPMD) operation is developed. The term perfect implies that no memory fragmentation occurs and ensures that the memory map size is kept to a minimum. A major constraint on solving this problem is based on the single program nature of both the SIMD and SPMD modes of parallelism. It is assumed that all processors within the same submachine used identical addresses to access corresponding data items in each of their local memories. Necessary and sufficient conditions are derived for being able to create perfect memory maps, and results are applied to several partitionable interconnection networks  相似文献   
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