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31.
Our previous reports have shown that two thirds of 4-nitroquinoline-1-oxide (4NQO)-induced murine oral squamous cell carcinomas (SCC) have Hras1 mutations. Loss of heterozygosity (LOH) involving the distal portion of chromosome (Chr) 7 occurred in half of the tumors with Hras1 mutations. Here, we demonstrate that five of six tumors with LOH have 4-8-fold amplification involving the distal portion of Chr 7 (7F4). Ccnd1. Fgf4 and Fgf3, within the most telomeric region of Chr 7 (70.5 cM), are co-amplified. The region is syntenic to a previously identified human amplicon at 11q13. Only one out of eight tumors without LOH at Chr 7 had twofold amplification; the other seven had no detectable amplification. Significant amplification is restricted to the chromosome with the Hras1 mutation. Gene amplification occurred without overexpression since only one of five tumors with amplification and one of six tumors without Ccnd1 amplification expressed increased protein. Although amplification of 11q13 occurs rather frequently in human tumors, 4NQO-induced oral cavity tumors in inbred mice are the first example of a murine tumor with consistent amplification. Our observations are strikingly similar to human head and neck SCC where overexpression of genes within the 11q13 amplicon is inconsistently detected. The amplification of genes localized to human 11q13 and the syntenic region on murine Chr 7 during tumorigenesis suggests that similar structural elements are present which predispose these regions to amplification during malignant transformation. 相似文献
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Habib M. Ammari 《Computer Networks》2012,56(7):1935-1950
The problem of sensor deployment to achieve k-coverage of a field, where every point is covered by at least k sensors, is very critical in the design of energy-efficient wireless sensor networks (WSNs). It becomes more challenging in mission-oriented WSNs, where sensors have to move in order to k-cover a region of interest in the field. In this type of network, there are multiple missions (or monitoring tasks) to be accomplished, each of which has different requirements, particularly, in terms of coverage. In this paper, we consider the problem of k-coverage in mission-oriented mobile WSNs which we divide into two sub-problems, namely sensor placement and sensor selection. The sensor placement problem is to identify a subset of sensors and their locations in a region of interest so it is k-covered with a small number of sensors. The sensor selection problem is to determine which sensors should move to the above-computed locations in the region while minimizing the total energy consumption due to sensor mobility and communication. Specifically, we propose centralized and distributed approaches to solve the k-coverage problem in mission-oriented mobile WSNs. Our solution to the sensor placement problem is based on Helly’s Theorem and the geometric analysis of the Reuleaux triangle. First, we consider a deterministic (or disk) sensing model, where the sensing range is modeled as a disk. Then, based on the above analysis, we address the k-coverage problem using a more realistic sensing model, known as probabilistic sensing model. The latter reflects the stochastic nature of the characteristics of the sensors, namely sensing and communication ranges. Our centralized and distributed protocols enable the sensors to move toward a region of interest and k-cover it with a small number of sensors. Our experiments show a good match between simulation and analytical results. In particular, simulation results show that our solution to the k-coverage problem in mission-oriented mobile WSNs outperforms an existing one in terms of the number of sensors needed to k-cover a region of interest in the field and their total energy consumption due to communication, sensing, and mobility for the correct operation of the protocol. 相似文献
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Sitai Liang Bijaya K. Nayak Kristine S. Vogel Samy L. Habib 《International journal of molecular sciences》2021,22(8)
The role of tumor protein 63 (TP63) in regulating insulin receptor substrate 1 (IRS-1) and other downstream signal proteins in diabetes has not been characterized. RNAs extracted from kidneys of diabetic mice (db/db) were sequenced to identify genes that are involved in kidney complications. RNA sequence analysis showed more than 4- to 6-fold increases in TP63 expression in the diabetic mice’s kidneys, compared to wild-type mice at age 10 and 12 months old. In addition, the kidneys from diabetic mice showed significant increases in TP63 mRNA and protein expression compared to WT mice. Mouse proximal tubular cells exposed to high glucose (HG) for 48 h showed significant decreases in IRS-1 expression and increases in TP63, compared to cells grown in normal glucose (NG). When TP63 was downregulated by siRNA, significant increases in IRS-1 and activation of AMP-activated protein kinase (AMPK (p-AMPK-Th172)) occurred under NG and HG conditions. Moreover, activation of AMPK by pretreating the cells with AICAR resulted in significant downregulation of TP63 and increased IRS-1 expression. Ad-cDNA-mediated over-expression of tuberin resulted in significantly decreased TP63 levels and upregulation of IRS-1 expression. Furthermore, TP63 knockdown resulted in increased glucose uptake, whereas IRS-1 knockdown resulted in a decrease in the glucose uptake. Altogether, animal and cell culture data showed a potential role of TP63 as a new candidate gene involved in regulating IRS-1 that may be used as a new therapeutic target to prevent kidney complications in diabetes. 相似文献
37.
In this study, we investigate a novel metallic microgripper which is able to grasp and transport nano particles (nano tubes/wires) and release them on desirable substrate by vibrating the gripper arms. This microgripper consists of a chevron actuator to grip nano object electrothermally and interdigited comb drive systems to generate vibration at the gripper arms electrostatically. Metallic (nickel) properties enable the chevron actuator to close the gap and pick the nano particle at low voltage and temperature. In order to reduce the out of plane bending during operation and also increase the gripping force, thickness of the nickel layer must be increased, hence electroplating process is proposed for deposition of nickel layer. To generate vibration at the end effectors, comb drive systems are stimulated by applying two voltage signals at desired resonant frequency to the stators. Practically, by sweeping the frequency of these signals around the resonant frequency the end effectors start vibrating. The vibration results in overcoming the adhesion forces due to inertial effects. 相似文献
38.
A. M. Mance M. A. Habib S. P. Maheswari S. J. Simko 《Journal of Applied Electrochemistry》1992,22(6):501-505
This paper describes a simple and inexpensive metallo-organic deposition (MOD) process for forming electrochromic tungsten oxide (WO3) films on glass. The thin films of WO3 were made by air firing (500–700°C) films from xylene/2-propanol solutions of bis-(di-n-octylammonium) tetratungstate, [(n-C8H17)2NH2]2[W4O13]. The process coats glass with undoped films ranging in colour from faint yellow to dark brown, and can be used to make gradients of these colours. The colour is determined by the firing parameters and results from residual carbon and tungsten suboxides in the film due to incomplete firing. Increased firing temperatures or longer firing times removes the carbon and produces films with higher crystallinity. Electrochemical doping with acid (H+) switches the colour gradient films to a uniformly blue colour. 相似文献
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Mariem Nafti Chiraz Hannachi Jaber Hsaini Béchir Hamrouni Habib Nouaïgui 《分离科学与技术》2020,55(15):2772-2778
ABSTRACT A new approach of cloud point extraction CPE procedure is optimized for hexavalent chromium determination in airborne dusts. Triton X-114 is used as a surfactant and 1-(2-pyridylazo)-2-naphthol as a specific complexing agent for the trivalent chromium’s removal from the aqueous phase to isolate hexavalent chromium compounds. The parameters influencing the extraction protocol (pH, surfactant concentration, and temperature are optimized. The obtained detection and quantification limits are 0.1 and 0.4 μg/L, respectively. The linearity is verified, with a regression coefficient close to 0.999 and the extraction recovery exceeds 99%. The method was successfully applied to analyze airborne samples collected from workplaces. 相似文献
40.
Jadoon Habib Ullah Khan Huang Deqing Qin Na Gong Zifeng 《International Journal of Control, Automation and Systems》2021,19(8):2769-2783
International Journal of Control, Automation and Systems - The fault and disturbances estimation has an important role in the modern traction railway system. This paper proposes a unique method for... 相似文献