HSPA4 Is a Biomarker of Placenta Accreta and Enhances the Angiogenesis Ability of Vessel Endothelial Cells

Placenta accreta spectrum (PAS) accounts for 7% of maternal mortality and is associated with intraoperative and postoperative morbidity caused by massive blood loss, infection, and adjacent organ damage. The aims of this study were to identify the protein biomarkers of PAS and to further explore their pathogenetic roles in PAS. For this purpose, we collected five placentas from pregnant subjects with PAS complications and another five placentas from normal pregnancy (NP) cases. Then, we enriched protein samples by specifically isolating the trophoblast villous, deeply invading into the uterine muscle layer in the PAS patients. Next, fluorescence-based two-dimensional difference gel electrophoresis (2D-DIGE) and MALDI-TOF/MS were used to identify the proteins differentially abundant between PAS and NP placenta tissues. As a result, nineteen spots were determined as differentially abundant proteins, ten and nine of which were more abundant in PAS and NP placenta tissues, respectively. Then, specific validation with western blot assay and immunohisto/cytochemistry (IHC) assay confirmed that heat shock 70 kDa protein 4 (HSPA4) and chorionic somatomammotropin hormone (CSH) were PAS protein biomarkers. Further tube formation assays demonstrated that HSPA4 promoted the in vitro angiogenesis ability of vessel endothelial cells, which is consistent with the in vivo scenario of PAS complications. In this study, we not only identified PAS protein biomarkers but also connected the promoted angiogenesis with placenta invasion, investigating the pathogenetic mechanism of PAS.     

Synthesis, characterization and osteoconductivity properties of bone fillers based on alendronate-loaded poly(ε-caprolactone)/hydroxyapatite microspheres

A superior drug controlled release system capable of achieving efficient osteogenesis is in imperative demand because of limited bone substitute tissue for the treatment of bone defect. In the present study, we investigated the potential of using poly(ε-caprolactone)–hydroxyapatite (PCL–HA) composite microspheres as an injectable bone repair vehicle by controlled release of alendronate (AL), a medicine that belongs to the bisphosphonates family. The PCL/HA–AL microspheres were prepared with solid/oil/water emulsion technique, which included two processes: (1) AL was loaded on the hydroxyapatite nanoparticles; (2) the HA–AL complex was built in the PCL matrix. The spherical PCL/HA–AL microspheres were characterized with its significantly improved encapsulation efficiency of hydrophilic AL and better sustained release. Human bone mesenchymal stem cells (hMSCs) were cultured on the surface of these microspheres and exhibited high proliferative profile. Specifically, in osteogenic medium, hMSCs on the surface of PCL/HA–AL microspheres displayed superior osteogenic differentiation which was verified by alkaline phosphatase activity assay. In conclusion, by presenting strong osteogenic commitment of hMSCs in vitro, the PCL/HA–AL microspheres have the potential to be used as an injectable vehicle for local therapy of bone defect.     

水利综合类工程财务会计管理制度研究

水利工程多是水利综合类工程。目前多数水利综合类工程实行水利工程管理单位财务会计制度。随着我国社会主义市场经济体制的逐步建立,现行的水利工程管理单位财务会计制度已不适应国家财政三项制度改革和水管体制改革要求,需要进行财务管理制度创新,以促进水利综合类工程实现良性运行。     

基于卡尔曼滤波的蠕虫检测方法

蠕虫对Internet安全构成了严重威胁,检测和防范蠕虫成为网络安全的研究课题.提出了一种基于卡尔曼滤波的蠕虫检测方法,建立适当的数学模型,给出相应的滤波方程,最后进行仿真分析.该方法可以利用实时量测信息不断地修正估计值.仿真结果表明,采用卡尔曼滤波能够快速有效地检测出蠕虫的爆发.     

基于Peercast的P2P直播系统改进

分析Peercast流媒体直播系统的结构。根据分析结果,指出其在实际网络应用中的弊端。改进下载流程,将单线程下载改为多线程下载,设计多线程F载任务分配模块。重新设计缓冲区,维护多对一情况F的缓冲区。介绍在多线程下载情况下直播延时方面的改进,对改进方案的性能进行分析。     

一种快速提取建筑物高度的方法研究

目前有多种利用单张高分辨率卫星影像计算建筑物高度的模型,但是其适用条件限制了建筑物高度提取的速度和精度。为了解决这一难题,提出利用建筑物投影同名点线段长度估算高度方法,并结合高分辨率影像数据和实测建筑物高度数据对估算结果进行分析。结果表明计算模型正确、精度较高(绝对误差在2.04 m以内)、使用参数较少、普适性强,在城市灾害评估与预测方面具有较大的实用潜质。     

QoS-aware placement of stream processing service

Many emerging online stream processing services require the consideration of quality of service (QoS), which is highly dependent on the placement of services at various hosts. This paper investigates the QoS-aware placement problems of stream processing services under different contexts. On condition that the client demands are stable, the QoS-aware placement problem aiming to minimize the cost when servers are CPU-uncapacitated, is equivalent to the set cover problem, and can be solved by a greedy algorithm with approximation factor O(log?n), where n is the number of clients. However, when CPU capacity constraints on servers are taken into account, the QoS-aware placement problem cannot be approximated unless P=NP. Therefore, we propose two heuristic algorithms: (1) ISCA (Iterated Set Cover-based Algorithm) and (2) KBA (Knapsack-Based Algorithm). We also consider the placement problem of client demands increasing over time. Two objectives, called extension factor and system lifetime, are proposed for demand increment-blind and increment-aware models respectively. Both of them can be solved by extending ISCA and KBA. The experimental results show that ISCA and KBA have distinct effects on different demand sizes. ISCA is more efficient when client demands are relatively small, while KBA performs better for larger demands.     

基于分段地址结构的快速路由查找算法

根据IPV6地址结构和骨干路由表特点,分析了原有路由查找算法,基于IPV6的掩码长度和分段地址,采用Hash表和多分支Trie树结构,提出了一种快速的IPV6路由查找算法。根据分段地址和掩码将最常用到的路由前缀按前缀长度设置Hash表,并将前缀值有序存放在表结点中。不仅可以进行前缀长度的二分查找,同时又是其它前缀匹配的索引。对于其他的前缀匹配问题,根据Hash表中的索引到相应的多分支Trie树完成最长前缀匹配。实践证明该算法具有较好的时空效率,可以较好地提高路由查找速度。     

高压直流换流变压器暂态铁心饱和不稳定及抑制

高压直流(HVDC)的铁心饱和谐波不稳定按照其激励源可划分为自发铁心饱和谐波不稳定和暂态铁心饱和谐波不稳定两种形式。在分析产生暂态铁心饱和谐波不稳定形成机理的基础上,提出暂态铁心饱和谐波不稳定新抑制措施,即整流侧接入SVC。且以云广特高压孤岛10%功率运行方式,采用PSCAD建立详细的电磁暂态模型,通过时域仿真试验验证了对暂态铁心饱和不稳定形成机理分析的正确性以及所提出整流侧接入SVC抑制暂态铁心饱和不稳定的有效性。     

基于闭环矢量法的六足机器人关节位姿研究

设计出了一种新型的三自由度缩放式的并联机构,并把该机构作为六足步行机器人的腿机构.采用闭环矢量法求解腿机构运动位置的正运动学问题,用MATLAB软件编写函数来求解腿机构的最大腿行程和最大抬跨高度/研究表明,原动件较小的位移可以获得较大的足端运动空间.