Involvement of MIIC-like late endosomes in B cell receptor-mediated antigen processing in murine B cells

Currently, the involvement of classical vs novel endocytic compartments in the phenomenon of B cell receptor (BCR)-mediated Ag processing is a matter of considerable debate. In murine B cells, class II vesicles (CIIV) represent a novel endocytic compartment involved in BCR-mediated Ag processing and class II peptide loading. Alternatively, in human B cells, the MHC class II-enriched compartment (MIIC) represents a lysosome (L)-like endocytic compartment that appears to be involved in this process. Presently, the relationship between CIIV, MIIC, and classical endosomes and L remains to be determined. Using density gradient centrifugation, a subcellular compartment morphologically and immunologically similar to human MIIC has been identified, isolated, and characterized in murine B cells. These MIIC-like vesicles represent a population of class II-positive late endosomes (LE) and are distinct from CIIV. MIIC-like LE are uniquely marked by the thiol protease cathepsin B, and along with mature L, appear to be the major repository of DM molecules in these cells. Importantly, both MIIC-like LE and CIIV isolated from Ag-pulsed B cells contain BCR-internalized Ag as well as antigenic peptide-class II complexes.     

Neural ensemble coding of target identity in echolocating bats

The Volvo plant at Olofstr?m, Sweden is engaged in the pressing and assembling of sheet-metal components for automobiles. During the past 25 years, determined efforts have been made to introduce less noisy methods of production and to limit the levels of noise emission in three large workshops at the plant. Noise levels were mapped with the aid of stationary noise measurements. The results are expressed in terms of noise emission zones and presented as noise contour maps. The investigations were made on four occasions between 1967 and 1991. Following the comparative area calculations of the noise emission zones, it was deduced that the noise levels had been significantly reduced, despite an increased production. The results show that noise reduction measures applied to the presses. An increase in automation in sheet-metal handling, improved quality in the pressing of components and changes in the range of products have contributed to the reduction of noise levels at the plant.     

The assessment of depression in the postnatal period: a comparison of four self-report questionnaires

OBJECTIVE: The objective of this study was to ascertain the degree of agreement between four self-report depression scales, with particular emphasis on whether each scale would identify the same subgroup of women as being 'most depressed'. METHOD: The questionnaires were administered to a sample of approximately 200 postnatal women at 4 weeks, 4 months and 8 months after delivery. The instruments were: the Edinburgh Postnatal Depression Scale; the depression subscale of the Hospital Anxiety Depression Scale; the Zung Self-Rating Depression Scale; and the depression subscale of the Profile of Mood States. RESULTS: Agreement between pairs of instruments, in terms of identifying the most depressed subgroup of women in the cohort, only averaged approximately 40%. Agreement between the three instruments was only about 25%. CONCLUSIONS: This poor level of agreement most likely reflects the different emphasis in item content of the questionnaires, which in turn reflects different notions of 'depression' held by the designers of the instruments. The implications of the findings for research and clinical practice are discussed.     

Specifying and verifying a broadcast and a multicast snooping cachecoherence protocol

We develop a specification methodology that documents and specifies a cache coherence protocol in eight tables: the states, events, actions, and transitions of the cache and memory controllers. We then use this methodology to specify a detailed, modern three-state broadcast snooping protocol with an unordered data network and an ordered address network that allows arbitrary skew. We also present a detailed specification of a new protocol called multicast snooping (Bilir et al., 1999) and, in doing so, we better illustrate the utility of the table-based specification methodology. Finally, we demonstrate a technique for verification of the multicast snooping protocol, through the sketch of a manual proof that the specification satisfies a sequentially consistent memory model     

Three Faces of Eve revisited: A study of transient microstrabismus.

Examined frame by frame C. H. Thigpen and H. Cleckley's film of a patient with multiple personality. Each of the 3 personalities of the S emerged in this film. A microtransient strabismus was detected and described, and its occurrence in relation to each of the 3 personalities analyzed. Eve Black, the least stable personality, exhibited 5 times as many of these strabismic occurrences as the other 2 personalities. A somewhat similar strabismus has also been observed in some films of schizophrenic patients, but has not been observed thus far in films of normal behavior. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Ascorbic Acid as an Adjuvant to Unbleached Cotton Promotes Antimicrobial Activity in Spunlace Nonwovens

The development of affordable, effective, and environmentally friendly barrier fabrics is a current goal in antimicrobial textile development. The discovery of new routes to achieve non-toxic naturally occurring molecules with antimicrobial activity is of interest in the development of materials that promote wound healing, improve hygiene, and offer protection against nosocomial infection. Highly cleaned and sterile unbleached cotton has constituents that produce hydrogen peroxide at levels commensurate with those that favor cell signaling in wound healing. Here, we show the antimicrobial and antiviral properties of spunlaced griege cotton-containing nonwovens treated with ascorbic acid formulations. The mechanism of action occurs through the promotion of enhanced hydrogen peroxide activity. The levels of hydrogen peroxide activity afford antimicrobial activity against Gram-negative and Gram-positive bacteria and antiviral activity against MS2 bacteriophages. Spun-bond nonwoven unbleached cotton was treated with ascorbic acid using traditional pad-dry-cure methods. An assessment of antibacterial and antiviral activity against Staphylococcus aureus, Klebsiella pneumoniae, and MS2 bacteriophages with the AATCC 100 test method showed a 99.99% inhibitory activity. An approach to the covalent attachment of ascorbic to cellulose through citric acid crosslinking chemistry is also discussed. Thus, a simple, low-cost approach to antimicrobial and antiviral cotton-based nonwovens applicable to dressings, nosocomial barrier fabrics, and face masks can be adopted by combining ascorbic acid with spunlace greige cotton nonwoven fabrics.     

Increased Angiogenesis and Lymphangiogenesis in Adenomyosis Visualized by Multiplex Immunohistochemistry

There is evidence for increased angiogenesis in the (ectopic) endometrium of adenomyosis patients under the influence of vascular endothelial growth factor (VEGF). VEGF stimulates both angiogenesis and lymph-angiogenesis. However, information on lymph vessels in the (ectopic) endometrium of adenomyosis patients is lacking. In this retrospective matched case-control study, multiplex immunohistochemistry was performed on thirty-eight paraffin embedded specimens from premenopausal women who had undergone a hysterectomy at the Amsterdam UMC between 2001 and 2018 to investigate the evidence for (lymph) angiogenesis in the (ectopic) endometrium or myometrium of patients with adenomyosis versus controls with unrelated pathologies. Baseline characteristics of both groups were comparable. In the proliferative phase, the blood and lymph vessel densities were, respectively, higher in the ectopic and eutopic endometrium of patients with adenomyosis than in the endometrium of controls. The relative number of blood vessels without α-smooth muscle actinin (α SMA) was higher in the eutopic and ectopic endometrium of adenomyosis patients versus controls. The level of VEGF staining intensity was highest in the myometrium but did not differ between patients with adenomyosis or controls. The results indicate increased angiogenesis and lymphangiogenesis in the (ectopic) endometrium affected by adenomyosis. The clinical relevance of our findings should be confirmed in prospective clinical studies.     

System availability monitoring

A process set up by Digital to monitor and quantify the availability of its systems is described. The reliability data are collected in an automated manner and stored in a database. The breadth of data gathered provides a unique opportunity to correlate hardware andsoftware failures. In addition, several hypotheses have been tested, e.g. the relationship between crash rate and system load, the interdependence of crashes, the cause of crashes, and the effect of new releases in the operating system. It is concluded that the process (in operation since 1988) has yielded worthwhile information on the products monitored. The usual availability metrics are calculated regularly for the machines monitored. Trends in system fault occurrence have been identified, leading to suggestions for both software and hardware improvements. The monitoring process and analysis methodology are revised on an ongoing basis to improve the quality of information obtained and to extend the analysis to Digital's new systems. The recently announced VAX9000 mainframe and fault-tolerant VAXft 3000 are two such systems