Finite element simulations of crack propagation in functionally graded materials under flexural loading

Cracks in stepped and continuously graded material specimens under flexural loading were investigated via finite element analysis. Calculation of mechanical energy release rates and propagation angles with crack-opening displacement correlation and the local symmetry (K_II = 0) criterion, respectively, provided results most efficiently and accurately, as compared with compliance and J-integral approaches and other deflection criteria. A routine was developed for automatic crack extension and remeshing, enabling simulation of incremental crack propagation. Effects of gradient profile and crack geometry on crack-tip stresses and crack propagation path are examined, and implications of these for optimal design of graded components against failure by fast fracture are discussed.     

Measurement methods for LCoS displays

Abstract— Two measurement methods to characterize uniformity and the absolute cell gap of LCoS images will be reviewed. These are simple to use in a production environment and have allowed major improvements in manufacture to be achieved. For the purpose of this paper, the liquid‐crystal mode used is the 45° normally black mode.     

Dennett Explained?: A Critical Study of Dahlbom's Dennett and His Critics

Minds and Machines -     

Slicing Software for Model Construction

Applying finite-state verification techniques (e.g., model checking) to software requires that program source code be translated to a finite-state transition system that safely models program behavior. Automatically checking such a transition system for a correctness property is typically very costly, thus it is necessary to reduce the size of the transition system as much as possible. In fact, it is often the case that much of a program's source code is irrelevant for verifying a given correctness property.In this paper, we apply program slicing techniques to remove automatically such irrelevant code and thus reduce the size of the corresponding transition system models. We give a simple extension of the classical slicing definition, and prove its safety with respect to model checking of linear temporal logic (LTL) formulae. We discuss how this slicing strategy fits into a general methodology for deriving effective software models using abstraction-based program specialization.     

Identifying cell-to-cell variability in internalization using flow cytometry

Biological heterogeneity is a primary contributor to the variation observed in experiments that probe dynamical processes, such as the internalization of material by cells. Given that internalization is a critical process by which many therapeutics and viruses reach their intracellular site of action, quantifying cell-to-cell variability in internalization is of high biological interest. Yet, it is common for studies of internalization to neglect cell-to-cell variability. We develop a simple mathematical model of internalization that captures the dynamical behaviour, cell-to-cell variation, and extrinsic noise introduced by flow cytometry. We calibrate our model through a novel distribution-matching approximate Bayesian computation algorithm to flow cytometry data of internalization of anti-transferrin receptor antibody in a human B-cell lymphoblastoid cell line. This approach provides information relating to the region of the parameter space, and consequentially the nature of cell-to-cell variability, that produces model realizations consistent with the experimental data. Given that our approach is agnostic to sample size and signal-to-noise ratio, our modelling framework is broadly applicable to identify biological variability in single-cell data from internalization assays and similar experiments that probe cellular dynamical processes.     

Direct sampling of chemical weapons in water by photoionization mass spectrometry

The vulnerability of water supplies to toxic contamination calls for fast and effective means for screening water samples for multiple threats. We describe the use of photoionization (PI) mass spectrometry (MS) for high-speed, high-throughput screening and molecular identification of chemical weapons (CW) threats and other hazardous compounds. The screening technology can detect a wide range of compounds at subacute concentrations with no sample preparation and a sampling cycle time of approximately 45 s. The technology was tested with CW agents VX, GA, GB, GD, GF, HD, HN1, and HN3, in addition to riot agents and precursors. All are sensitively detected and give simple PI mass spectra dominated by the parent ion. The target application of the PI MS method is as a routine, real-time early warning system for CW agents and other hazardous compounds in air and in water. In this work, we also present comprehensive measurements for water analysis and report on the system detection limits, linearity, quantitation accuracy, and false positive (FP) and false negative rates for concentrations at subacute levels. The latter data are presented in the form of receiver operating characteristic curves of the form of detection probability P(D) versus FP probability P(FP). These measurements were made using the CW surrogate compounds, DMMP, DEMP, DEEP, and DIMP. Method detection limits (3sigma) obtained using a capillary injection method yielded 1, 6, 3, and 2 ng/mL, respectively. These results were obtained using 1-microL injections of water samples without any preparation, corresponding to mass detection limits of 1, 6, 3, and 2 pg, respectively. The linear range was about 3-4 decades and the dynamic range about 4-5 decades. The relative standard deviations were generally <10% at CW subacute concentrations levels.     

Tumor-Localized Administration of α-GalCer to Recruit Invariant Natural Killer T Cells and Enhance Their Antitumor Activity against Solid Tumors

Invariant natural killer T (iNKT) cells have the capacity to mount potent anti-tumor reactivity and have therefore become a focus in the development of cell-based immunotherapy. iNKT cells attack tumor cells using multiple mechanisms with a high efficacy; however, their clinical application has been limited because of their low numbers in cancer patients and difficulties in infiltrating solid tumors. In this study, we aimed to overcome these critical limitations by using α-GalCer, a synthetic glycolipid ligand specifically activating iNKT cells, to recruit iNKT to solid tumors. By adoptively transferring human iNKT cells into tumor-bearing humanized NSG mice and administering a single dose of tumor-localized α-GalCer, we demonstrated the rapid recruitment of human iNKT cells into solid tumors in as little as one day and a significantly enhanced tumor killing ability. Using firefly luciferase-labeled iNKT cells, we monitored the tissue biodistribution and pharmacokinetics/pharmacodynamics (PK/PD) of human iNKT cells in tumor-bearing NSG mice. Collectively, these preclinical studies demonstrate the promise of an αGC-driven iNKT cell-based immunotherapy to target solid tumors with higher efficacy and precision.     

Preface: Volume 70, Issue 3

This volume contains the Proceedings of the REFINE 2002 workshop. The Workshop was held in Copenhagen, Denmark on July 20 and 21, 2002, as a satellite event to FLoC'02 as an FME-affiliated workshop.Refinement is one of the cornerstones of a formal approach to software engineering. Refinement is the process of developing a more detailed design or implementation from an abstract specification through a sequence of mathematically-based steps that maintain correctness with respect to the original specification.The aim of this BCS FACS refinement workshop was to bring together people who are interested in the development of more concrete designs or executable programs from abstract specifications using formal notations, tool support for formal software development, and practical experience with formal refinement methodologies.The purpose of the workshop was to provide a forum for discussion of common ground and key differences. Topics of interest included:

• Simulation techniques

• Foundations and semantics

• Case studies (specification and verification)

• Compositional and modular reasoning

• Object-orientation

• Time

• Specification notations

• Programming models

• Verification and tool support

The workshop continued a long tradition of refinement workshops run under the auspices of the British Computer Society (BCS) FACS special interest group. Running since 1988, previous refinement workshops have been held at Cambridge, London, Bath etc.In 1998 the BCS refinement workshop was combined with the Australasian Refinement Workshop to form the International Refinement Workshop, hosted at alongside Formal Methods Pacific 1998 at The Australian National University.The papers in this volume were reviewed by a small program committee consisting of

•

John Derrick<

jd1@ukc.ac.uk

, University of Kent, UK.

•

Eerke Boiten<

eab2@ukc.ac.uk

, University of Kent, UK.

•

Jim Woodcock<

jcpw@ukc.ac.uk

, University of Kent, UK.

•

Joakim von Wright<

jockum.wright@abo.fi

, Åbo Akademi University, Finland

Additional information about the Workshop can be found at: http://www.cs.ukc.ac.uk/people/staff/eab2/refine/floc.htmlThis volume will be published as volume 70 issue 3 in the series Electronic Notes in Theoretical Computer Science (ENTCS). This series is published electronically through the facilities of Elsevier Science B.V. and its auspices. The volumes in the ENTCS series can be accessed at the URLhttp://www.elsevier.nl/locate/entcsA printed version of the current volume was distributed to the participants at the workshop in Denmark.We are very grateful to the following persons, whose help has been crucial for the success of REFINE 2002: Lars-Henrik Eriksson and Peter Lindsay of FME for their help with the organization of the Workshop as satellite event of FLoC'02 and an FME affiliated workshop; Mike Mislove, one of the Managing Editors of the ENTCS series, for his assistance with the use of the ENTCS style files. Thanks are also due to the Computing Laboratory of the University of Kent, which supplied financial support to cover the printing costs.July 24, 2002John Derrick