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91.
92.
Solid-phase extraction microtips are important devices in modern bioanalytics, as they allow miniaturized sample preparation for mass spectrometric analysis. Here we introduce the use of cotton wool for the preparation of filter-free HILIC SPE microtips. To this end, pieces of cotton wool pads (approximately 500 μg) were packed into 10 μL pipet tips. The performance of the tips was evaluated for microscale purification of tryptic IgG Fc N-glycopeptides. Cotton wool HILIC SPE microtips allowed the removal of salts, most nonglycosylated peptides, and detergents such as SDS from glycoconjugate samples. MALDI-TOF-MS glycopeptide profiles were very repeatable with different tips as well as reused tips, and very similar profiles were obtained with different brands of cotton wool pads. In addition, we used cotton HILIC microtips to purify N-glycans after N-glycosidase F treatment of IgG and transferrin followed by MALDI-TOF-MS detection. In conclusion, we establish cotton wool microtips for glycan and glycopeptide purification with subsequent mass spectrometric detection. 相似文献
93.
Maurice L. Huggins K.uan-Ran Sun Alexander Silverman 《Journal of the American Ceramic Society》1943,26(12):393-398
The difficulty of defining the vitreous state in a rigorous manner, the existence of many borderline cases, and the theoretical requirements which must be met if a substance is to be vitreous are discussed. Silicate glasses and certain other typical classes of vitreous substances are treated in some detail with especial reference to the relation between their structures and vitreous properties. 相似文献
94.
Scientometrics - The h-index is the most used measurement of impact for researchers. Sites such as Web of Science, Google Scholar, Microsoft Academic, and Scopus leverage it to show and compare the... 相似文献
95.
Szymusiak Ronald; McGinty Dennis; Shouse Margaret N.; Shepard Diane; Sterman Maurice B. 《Canadian Metallurgical Quarterly》1990,104(1):217
Sensorimotor electroencephalogram (EEG) frequencies in cats were evaluated with power spectral analysis before and after 3 doses of atropine sulfate. All doses of atropine tested caused enhanced EEG slow waves (0–7 Hz) and spindles (8–25 Hz) during waking immobility, and postdrug frequency profiles during slow-wave sleep and waking immobility were identical. With 0.75 mg/kg atropine, movement (head movement, locomotion) resulted in EEG desynchronization and reduced power in all frequencies less than 24 Hz. After 1.5 or 3.0 mg/kg atropine, power in low frequencies remained elevated during movement, but power in spindle frequencies was significantly reduced compared with other states. During active REM sleep after 1.5 mg/kg atropine, power in spindle frequencies was significantly lower than that during quiet REM sleep. These results indicate that the sensorimotor cortical EEG in cats is under the control of multiple systems. At least 1 of these systems is active during movement, and its actions are resistant to muscarinic receptor blockade. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Peter F. Martin W. Allan Gillespie Maurice G. Kelliher 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》1985,240(2):445-451
A versatile electron spectrum monitor has been developed for the UK free electron laser project. The monitor uses secondary emission from 96 tungsten foils and microprocessor-controlled sampling electronics to produce a high-resolution electron energy spectrum. A 30-channel prototype is currently operating as a beam profile monitor and has been extensively used in emittance measurements at the Kelvin Laboratory linac. 相似文献
99.
The physics of mechanical alloying: A first report 总被引:14,自引:0,他引:14
In this paper, we present a first attempt to define the basic geometry, mechanics, and physics of the process of mechanical
alloying. The geometry of the collision events which lead to particle fragmentation and coalescence is modeled on the basis
of Hertzian contacts between the grinding media which entrap a certain amount of material volume between the impacting surfaces.
This geometry essentially defines the volume of material affected per collision, and from this information and characteristics
of the specific mill and the material being processed, impact times, powder strain rates and strains, powder temperature increase,
powder cooling times, and milling times can be approximated. 相似文献
100.