Fabrication of nanoconcave surface for cell immobilization in cell-based chip

Nanostructured surface such as nanoconcave shape can be utilized as a bioplatform to immobilize cells. In this study, we present fabrication of Au-coated nanoconcave surface and some possibility of cell immobilization. Long-range ordered periodic patterns with concave shape were formed on aluminum substrate by electrochemical anodization process. The morphology and topography of nanoconcave surface was investigated by atomic force microscopy and scanning electron microscopy. The pore-pore distance and the pore depth of nanoconcave pattern were measured at 105 +/- 5 nm and 30 +/- 2 nm, respectively. After Au deposition, the pore depth within Au-coated concave surface was 15 +/- 2 nm. The topography of HeLa cells immobilized on the nanoconcave surface was observed by AFM combined with confocal microscopy. The result expected that the Au-coated nanoconcave surface may be used as new culture substrate for cells immobilization in cell-based chip.     

Stochasticity and time delays in gene expression and evolutionary game theory

We discuss effects of stochasticity and time delays in simple models of population dynamics. In social-type models, where individuals react to the information concerning the state of the population at some earlier time, sufficiently large time delays may cause oscillations. In biological-type models, where some changes already take place in the population at an earlier time, oscillations might not be present for any time delay. We illustrate this idea in models of delayed random walks, gene expression, and population dynamics of evolutionary game theory.     

Continuous ethanol production from wood hydrolysate by chemostat and total cell retention culture

Chemostat and total cell retention cultures with internal filter system ofSaecharomyc.es cerevisiae H1-7 were carried out to produce ethanol from wood hydrolysate. Maximum ethanol productivity obtained in a chemostat with the aeration rate of 1 vvm was 3.79 g/(L·h). This was 20% higher than that in a chemostat without aeration. However, the substrate was not completely consumed at the dilution rate with the maximum productivity. The realistic productivity, which has higher than 99% conversion rate of substrate, was. 2.95 g/(L·h). The maximum productivity in the total cell retention culture was 6.65 g/(L·h) at the dilution rate of 0.19 h¹ and the residual glucose concentration was negligible.     

劣质粉煤灰的改性激活及高强度粉煤灰砌块的研究

介绍以劣质为主要原料,制备高强度粉煤灰硅酸盐砌块的研究成果。研究表明,采用磨细、添加化学性物质以及水热合成反应条件等激活方法可以提高粉煤灰的反应活性,进而显著提高粉煤灰砌块的早期强度。本文还对影响粉煤灰砌块强度的诸因素进行讨论,并根据粉煤灰结构特性,阐述激发方法和制备因素对粉煤灰砌块强度影响的作用机理。