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91.
Lamarque M Tastet C Poncet J Demettre E Jouin P Vial H Dubremetz JF 《Proteomics. Clinical applications》2008,2(9):1361-1374
The Plasmodium falciparum food vacuole (FV) is a lysosome-like organelle where erythrocyte hemoglobin digestion occurs. It is a favorite target in the development of antimalarials. We have used a tandem mass spectrometry approach to investigate the proteome of an FV-enriched fraction and identified 116 proteins. The electron microscopy analysis and the Western blot data showed that the major component of the fraction was the FV and, as expected, the majority of previously known FV markers were recovered. Of particular interest, several proteins involved in vesicle-mediated trafficking were identified, which are likely to play a key role in FV biogenesis and/or FV protein trafficking. Recovery of parasite surface proteins lends support to the cytostomal pathway of hemoglobin ingestion as a FV trafficking route. We have identified 32 proteins described as hypothetical in the databases. This insight into FV protein content provides new clues towards understanding the biological function of this organelle in P. falciparum. 相似文献
92.
Barelli S Canellini G Thadikkaran L Crettaz D Quadroni M Rossier JS Tissot JD Lion N 《Proteomics. Clinical applications》2008,2(2):142-157
Protein oxidation mechanisms result in a wide array of modifications, from backbone cleavage or protein crosslinking to more subtle modifications such as side chain oxidations. Protein oxidation occurs as part of normal regulatory processes, as a defence mechanism against oxidative stress, or as a deleterious processes when antioxidant defences are overcome. Because blood is continually exposed to reactive oxygen and nitrogen species, blood proteomics should inherently adopt redox proteomic strategies. In this review, we recall the biochemical basis of protein oxidation, review the proteomic methodologies applied to analyse redox modifications, and highlight some physiological and in vitro responses to oxidative stress of various blood components. 相似文献
93.
94.
95.
As a notion dual to Knuth's nested formulas [4], we call a boolean formula
in conjunctive normal formco-nested if its clauses can be linearly ordered (sayC={c
i
;i=1,2, ...,n})so that the graphG
cl
=(XC, {xc
i
;xc
i
or ¬xc
i
} {c
i
c
i+1;i=1, 2, ...,n}) allows a noncrossing drawing in the plane so that the circlec
1,c
2, ...,c
n
bounds the outerface. Our main result is that maximum satisfiability of co-nested formulas can be decided in linear time.Both authors acknowledge a partial support of Ec Cooperative Action IC-1000 (project ALTEC:Algorithms for Future Technologies). 相似文献
96.
Jean Mayrand Jean-François Patenaude Ettore Merlo Michel Dagenais Bruno Laguë 《Annals of Software Engineering》2000,9(1-2):117-141
This paper presents an assessment method to evaluate the quality of object oriented software systems. The assessment method is based on source code abstraction, object–oriented metrics and graphical representation. The metrics used and the underlying model representing the software are presented. The assessment method experiment is part of an industrial research effort with the Bell Canada Quality Engineering and Research Group. It helps evaluators assess the quality and risks associated with software by identifying code fragments presenting unusual characteristics. The assessment method evaluates object–oriented software systems at three levels of granularity: system level, class level and method level. One large C++ and eight Java software systems, for a total of over one million lines of code, are presented as case studies. A critical analysis of the results is presented comparing the systems and the two languages. 相似文献
97.
NMR measurements are reported on rotating3He-A in a long cylindrical geometry of 5 mm diameter at a liquid pressure of 29.3 bar and in axial magnetic fields of 14.2, 28.4, and 56.9 mT. At 28.4 mT, NMR studies are also reported in fields inclined by 25° and 90° from the axis of rotation. The frequency shift, the width, and the intensity of the spin wave modes localized on the soft vortex cores, as well as the additional broadening of the main NMR line during rotation, were measured as a function of temperature, angular velocity , magnetic field intensity, and its inclination angle. Also observed were a critical angular velocity of vortex formation, hysteretic behavior in the number of vortices when comparing accelerating rotation to decelerating, and metastable vortex densities, presumably a vortex tangle after rapid oscillatory acceleration. The results can be understood in terms of the continuous 4 vortices first proposed by Seppälä and Volovik. 相似文献
98.
This article presents the result of the current knowledge on the very high frequency electromagnetic radiation of lightning. A phenomenology analysis of the new results obtained from the study of triggered lightning in New Mexico (TRIP 82) and at Saint-Privat-d’ Allier in Haute-Loire, France (1983) is first described. The authors then present the spectral and temporal characteristics of the VHF/UHF radiation which can be applied to the electromagnetic compatibility of systems. From a synthesis of the experimental and theoretical contributions, they propose a reflection on the lightning initiation process and on the mechanisms generating lightning radiation. 相似文献
99.
This paper considers the problem of multirobot coordination in pick-and-place tasks on a conveyor band. The robot team is composed of identical robots with mutually exclusive, but neighboring workspaces. The products are fed in at one end of the band, move through each workspace sequentially until being picked up and are collected at the other end—if not picked up interim. Each robot has the same task—that is picking up and packaging as many products as possible. We propose an approach based on noncooperative game theory where each robot uses local observations of the conveyor band and their neighbors' actions in order to decide on its actions. The developed algorithm has been implemented and tested in a simulated manufacturing environment using Webots. Results obtained from the simulations are analyzed using a variety of statistical performance measures. 相似文献
100.
Darío MaravallAuthor Vitae Javier de LopeAuthor Vitae Raúl DomínguezAuthor Vitae 《Neurocomputing》2012,75(1):106-114
In multi-agent systems, the study of language and communication is an active field of research. In this paper we present the application of evolutionary strategies to the self-emergence of a common lexicon in a population of agents. By modeling the vocabulary or lexicon of each agent as an association matrix or look-up table that maps the meanings (i.e. the objects encountered by the agents or the states of the environment itself) into symbols or signals we check whether it is possible for the population to converge in an autonomous, decentralized way to a common lexicon, so that the communication efficiency of the entire population is optimal. We have conducted several experiments aimed at testing whether it is possible to converge with evolutionary strategies to an optimal Saussurean communication system. We have organized our experiments alongside two main lines: first, we have investigated the effect of the population size on the convergence results. Second, and foremost, we have also investigated the effect of the lexicon size on the convergence results. To analyze the convergence of the population of agents we have defined the population's consensus when all the agents (i.e. 100% of the population) share the same association matrix or lexicon. As a general conclusion we have shown that evolutionary strategies are powerful enough optimizers to guarantee the convergence to lexicon consensus in a population of autonomous agents. 相似文献