Identification and quantification of anammox bacteria in eight nitrogen removal reactors

Various studies have revealed anaerobic ammonium oxidation (anammox) as a very attractive alternative process suitable for nitrogen removal from wastewater. Here we investigated anammox bacteria in eight different nitrogen removal reactors. The diversity and abundance of anammox bacteria were determined by the 16S rRNA gene analysis, fluorescence in situ hybridization with specific probes and real-time quantitative PCR (qPCR). In these reactors, at least eight unique near full length anammox 16S rRNA gene sequences were detected, which were distributed over two genera; Candidati Brocadia and Kuenenia. FISH results confirmed that only one anammox bacterium dominated the community in each of the eight reactors investigated in this study. qPCR analysis revealed that anammox bacteria were present in seven of the reactors in the order of 10⁹ cells/ml and 10⁷ cells/ml in reactor A1. The dominant and divergent Brocadia-like anammox phylotype in one reactor represented a novel species for which we propose the name Candidatus Brocadia sinica. Taken together, these results indicated that a single seeding source could be used to seed anammox reactors designed to treat different types of wastewater, which could lead to a faster start-up of bioreactors.     

在LTspice/SwitcherCAD Ⅲ中采用变压器

关于LTspice/SwitcherCAD Ⅲ LTspice/SwitcherCAD Ⅲ是一款具集成原理图捕获能力的强大SPICE仿真工具.     

iFlash:80℃活化漂白与新型皂洗

加工技术与设备的发展通常会使加工时间缩短.但是新型助剂的应用也可减少加工时间,大大降低成本.随着iFlash系统的应用,漂白温度可降至80℃,活性染料染色后皂洗的时间也大大缩短.因此,织物间歇式染色得到优化,在不增加额外投资的情况下运行更经济.     

实现无线设计过程的无缝协作

(上篇)在无线系统设计过程中依靠基于部门划分的方法,往往会给开发流程带来麻烦。使用系统级模型不仅可以解决诸多此类问题,而且能够大大改进设计流程。各家公司都在使用基于模型的设计,以整合不同的开发团队(例如,模拟和数字)和各个设计阶段(例如,设计和验证),从而获得巨大收益。     

Vehicle mass and injury risk in two-car crashes: A novel methodology

This paper introduces a novel methodology based on disaggregate analysis of two-car crash data to estimate the partial effects of mass, through the velocity change, on absolute driver injury risk in each of the vehicles involved in the crash when absolute injury risk is defined as the probability of injury when the vehicle is involved in a two-car crash. The novel aspect of the introduced methodology is in providing a solution to the issue of lack of data on the speed of vehicles prior to the crash, which is required to calculate the velocity change, as well as a solution to the issue of lack of information on non-injury two-car crashes in national accident data. These issues have often led to focussing on relative measures of injury risk that are not independent of risk in the colliding cars. Furthermore, the introduced methodology is used to investigate whether there is any effect of vehicle size above and beyond that of mass ratio, and whether there are any effects associated with the gender and age of the drivers. The methodology was used to analyse two-car crashes to investigate the partial effects of vehicle mass and size on absolute driver injury risk. The results confirmed that in a two-car collision, vehicle mass has a protective effect on its own driver injury risk and an aggressive effect on the driver injury risk of the colliding vehicle. The results also confirmed that there is a protective effect of vehicle size above and beyond that of vehicle mass for frontal and front to side collisions.     

Dynamic construction site layout planning using max-min ant system

Construction site layout planning (CSLP) is a dynamic multi-objective optimization (MOO) problem as there are different facilities employed in the different construction phases of a construction project. In this study, a new method using continuous dynamic searching scheme to guide the max-min ant system (MMAS) algorithm, which is one of the ant colony optimization (ACO) algorithms, to solve the dynamic CSLP problem under the two congruent objective functions of minimizing safety concerns and reducing construction cost is proposed. Using weighted sum method the MOO problem can be solved by the proposed MMAS method. An office building case was used to verify the capability of the proposed method to solve dynamic CSLP problem and the results are promising. The approach could be benchmarked by researchers using other advanced optimization algorithms to solve the same problem or expand the applications to other fields.     

Effects of ionic group incorporation into model polypeptide,poly(γ-benzyl-L-glutamate) (PBLG)

Effects of incorporation of ionic groups and ionic interactions to a model polypeptide, poly(γ-benzyl-L-glutamate) (PBLG), are studied. Partial conversion of γ-benzyl-L-glutamate residues to glutamic acid residues does not change molecular conformation (α-helix) and shows little change in properties. By contrast, partial conversion to sodium glutamates (ionic groups) leads to conversion from a rigid rod to a semi-flexible rod at low ion contents and causes significant changes in thermal stability and birefringence. In solution, lyotropic liquid crystalline behavior of PBLG is lost at ion content as low as 3.2 mol %. In solid, birefringence is still observed at this ion content, suggesting thermotropic liquid crystallinity of this ionic PBLG. Molecular composites made of ionic PBLG as a reinforcer and poly(4-vinylpyridine) (PVP) as a matrix polymer show significant enhancement in strength and toughness as a result of ion-dipole interactions between the component polymers.