Catalase (CAT) Gene Family in Rapeseed (Brassica napus L.): Genome-Wide Analysis,Identification, and Expression Pattern in Response to Multiple Hormones and Abiotic Stress Conditions

Catalase (CAT) is an antioxidant enzyme expressed by the CAT gene family and exists in almost all aerobic organisms. Environmental stresses induce the generation of reactive oxygen species (ROS) that eventually hinder plant growth and development. The CAT enzyme translates the hydrogen peroxide (H₂O₂) to water (H₂O) and reduce the ROS levels to shelter the cells’ death. So far, the CAT gene family has not been reported in rapeseed (Brassica napus L.). Therefore, a genome-wide comprehensive analysis was conducted to classify the CAT genes in the rapeseed genome. The current study identified 14 BnCAT genes in the rapeseed genome. Based on phylogenetic and synteny analysis, the BnCATs belong to four groups (Groups I–IV). A gene structure and conserved motif analysis showed that Group I, Group II, and Group IV possess almost the same intron/exon pattern, and an equal number of motifs, while Group III contains diverse structures and contain 15 motifs. By analyzing the cis-elements in the promoters, we identified five hormone-correlated responsive elements and four stress-related responsive elements. Further, six putative bna-miRNAs were also identified, targeting three genes (BnCAT4, BnCAT6, and BnCAT8). Gene ontology (GO) enrichment analysis showed that the BnCAT genes were largely related to cellular organelles, ROS response, stimulus response, stress response, and antioxidant enzymes. Almost 10 BnCAT genes showed higher expression levels in different tissues, i.e., root, leaf, stem, and silique. The expression analysis showed that BnCAT1–BnCAT3 and BnCAT11–BnCAT13 were significantly upregulated by cold, salinity, abscisic acid (ABA), and gibberellic acid (GA) treatment, but not by drought and methyl jasmonate (MeJA). Notably, most of the genes were upregulated by waterlogging stress, except BnCAT6, BnCAT9, and BnCAT10. Our results opened new windows for future investigations and provided insights into the CAT family genes in rapeseed.     

特殊工业粉尘地区复合绝缘子腐蚀失效过程研究

硅橡胶复合绝缘子因其优异的憎水性及憎水迁移性而具备较好的防污闪能力,往往作为应对污闪事故的首选方案。但在部分特殊工业粉尘地区,复合绝缘子在短时间运行后会出现爬电及蚀损现象,并发展为绝缘失效。为研究特殊工业粉尘地区复合绝缘子的腐蚀失效过程,测试了某特殊工业园区内运行的复合绝缘子的表面污秽度及污秽成分、污闪电压梯度、憎水性能及微观性能,并分析了其腐蚀失效过程。主要结论如下:绝缘子表面等值盐密为0.1~0.2 mg/cm²,其污秽成分与化工污源类似;存在自然污秽时绝缘子表面憎水性良好,但其憎水性的减弱、恢复及迁移特性均不能满足运行要求;绝缘失效的起因是电晕放电导致憎水性降低,进而导致伞裙电蚀损,最后发展为绝缘失效。     

MICROSTRUCTURES AND TEXTURES IN COLD-ROLLED ALUMINIUM STRIP

ＭＩＣＲＯＳＴＲＵＣＴＵＲＥＳＡＮＤＴＥＸＴＵＲＥＳＩＮＣＯＬＤＲＯＬＬＥＤＡＬＵＭＩＮＩＵＭＳＴＲＩＰ①ＮｉｅＺｕｏｒｅｎ，ＺｈａｎｇＸｉｎｍｉｎｇ，ＹｉｎＺｈｉｍｉｎＤｅｐａｒｔｍｅｎｔｏｆＭａｔｅｒｉａｌｓＳｃｉｅｎｃｅａｎｄＥｎｇｉｎｅｒｉ...     

The effect of rate sensitivity on history dependent forming limits of anisotropic sheet metals

history dependent forming limits of sheet metals are computed by examining the role of rate sensitivity of flow stress in forming limits for anisotropic materials. Assuming that necking or nonuniform deformation is caused by initial heterogeneity, the forming limits are computed by considering the flow theory and incorporating an isotropic hardening model for anisotropic materials. Representative results show some of the trends for changing forming limits with different parameters under complex nonproportional loading histories. The computed results agree well with published experimental data for different loading history, anisotropy, and rate sensitivity of sheet materials.     

工业循环冷却水成套节水新技术在天津石化乙烯厂的应用

为解决天津地区水资源短缺的现状，通过应用先进的水处理药剂及自动监控技术，在天津石化公司乙烯厂成功地建成了高浓缩倍率冷却水处理技术示范工程，系统浓缩倍率从3倍提高到5倍以上，年节水量超过60万t。     

低浓度的阴离子洗涤剂实验室内的质量控制

在环境监测中低浓度的阴离子洗涤剂实验室内质量控制应采取以下措施：空白试验值测定，检出限的确定，空白试验的质量控制图，校准曲线的检验，标准样品的测定。     

聚乙烯转光棚膜的加工及应用研究

本文对聚乙烯转光棚膜的加工和光转换剂的作用、配比关系及物理性能进行了讨论。     

凉型风味料介绍

本文分析了主要凉味剂的立体构型 ,简单介绍了以L -薄荷脑 (又称L -薄荷醇 )和L -薄荷脑为基本骨架的酯类、醚类和缩酮类化合物的结构和味觉特征 ,它们的安全性以及在食品、口腔卫生用品、化妆品和肤用药中的应用。     

基于ANSYS混凝土重力坝流固耦合动力特性分析

以某混凝土重力坝非溢流坝段动力特性为背景,探讨流固耦合效应对结构自振特性的影响,运用大型有限元软件对结构自振特性进行分析计算,得出了流体对结构自振特性影响以及结构的固有频率,避免在运行过程中发生共振,造成对结构的破坏。     

Electrosyntheses and characterization of poly(5‐bromoindole) in boron trifluoride diethyl etherate

Poly(5‐bromoindole) (PBrI) films were synthesized electrochemically by direct oxidation of 5‐bromoindole in pure boron trifluoride diethyl etherate. The oxidation potential of 5‐bromoindole in this medium was measured to be only 0.97 V vs. saturated calomel electrode, which was lower than that determined in acetonitrile + 0.1 mol L^?1 Bu₄NBF₄ (1.08 V). PBrI films obtained from this medium showed good electrochemical behavior and good thermal stability. Structural studies showed that the polymerization of 5‐bromoindole ring occurred at 2,3 position. As‐formed PBrI films were thoroughly soluble in strong polar solvent dimethylsulfoxide and partly soluble in tetrahydrofuran. Fluorescent spectral studies indicated that PBrI was a good blue‐light emitter. The excitation and emission spectra of PBrI showed a significant shift to longer wavelength compared with that of the monomer, consistent with the greater extent of electron delocalization. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 101: 539–547, 2006