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131.
Alessandro Tocchio Naside Gozde Durmus Kaushik Sridhar Vigneshwaran Mani Bukre Coskun Rami El Assal Utkan Demirci 《Advanced materials (Deerfield Beach, Fla.)》2018,30(4)
In nature, cells self‐assemble at the microscale into complex functional configurations. This mechanism is increasingly exploited to assemble biofidelic biological systems in vitro. However, precise coding of 3D multicellular living materials is challenging due to their architectural complexity and spatiotemporal heterogeneity. Therefore, there is an unmet need for an effective assembly method with deterministic control on the biomanufacturing of functional living systems, which can be used to model physiological and pathological behavior. Here, a universal system is presented for 3D assembly and coding of cells into complex living architectures. In this system, a gadolinium‐based nonionic paramagnetic agent is used in conjunction with magnetic fields to levitate and assemble cells. Thus, living materials are fabricated with controlled geometry and organization and imaged in situ in real time, preserving viability and functional properties. The developed method provides an innovative direction to monitor and guide the reconfigurability of living materials temporally and spatially in 3D, which can enable the study of transient biological mechanisms. This platform offers broad applications in numerous fields, such as 3D bioprinting and bottom‐up tissue engineering, as well as drug discovery, developmental biology, neuroscience, and cancer research. 相似文献
132.
T. Venkata Deepthi Ch. Sridhar Reddy A. Satyadevi 《International Journal for Computational Methods in Engineering Science and Mechanics》2018,19(6):412-418
AbstractAn estimation of elastic-plastic stresses and strains is presented for mechanical components, using pseudo elastic analysis based on the deformation theory of plasticity. Analysis of two applications, one under proportional loading and other under non-proportional loading paths using pseudo elastic finite element method, is presented. A rectangular plate with a hole under tension loading and a rectangular plate fixed at one end under bending-tension non-proportional loading are considered for analysis. Pseudo Elastic finite element analysis for proportional loading uses elastic solutions and varies material properties for elements in plastic zone to estimate elastic-plastic solution. A finite element code is developed based on pseudo elastic analysis method. An attempt is made to extend pseudo elastic analysis to analyze bending-tension non-proportional loading problem. Both applications in consideration are assumed to be of Von Mises material and follow isotropic hardening rule with elastic-linear hardening material model. Non-linear analysis of the plate with a hole under proportional tensile loading and that of rectangular plate under bending-tension non-proportional loading are performed in ANSYS and results are compared for validation and are observed to be in good agreement with present analysis. 相似文献
133.
Concept learning in robotics is an extremely challenging problem: sensory data is often high-dimensional, and noisy due to specularities and other irregularities. In this paper, we investigate two general strategies to speed up learning, based on spatial decomposition of the sensory representation, and simultaneous learning of multiple classes using a shared structure. We study two concept learning scenarios: a hallway navigation problem, where the robot has to induce features such as opening or wall. The second task is recycling, where the robot has to learn to recognize objects, such as a trash can. We use a common underlying function approximator in both studies in the form of a feedforward neural network, with several hundred input units and multiple output units. Despite the high degree of freedom afforded by such an approximator, we show the two strategies provide sufficient bias to achieve rapid learning. We provide detailed experimental studies on an actual mobile robot called PAVLOV to illustrate the effectiveness of this approach. 相似文献
134.
The low-melting ternary sulfate and modified sulfate glasses based on transition metals like copper, nickel, and vanadium reduce the smoke generation during burning of the PVC compound. With increase in concentration of these glasses, the smoke-density rating values decrease without appreciably altering the oxygen index values. Although trihydrated alumina and potash alum improve flame-retarding properties, the latter cannot be used as it considerably reduces the thermal stability of the PVC compound. Formulations to meet the flame-retardant requirements of a PVC compound suitable for cable sheathing have been developed. 相似文献
135.
Alamanda V. Prasadarao Ulagaraj Selvaraj Sridhar Komarneni Amar S. Bhalla 《Journal of the American Ceramic Society》1992,75(10):2697-2701
Strontium and calcium pyroniobates were prepared by a sol–gel process, using strontium/calcium metal and niobium ethoxide as precursors. The formation of Sr2 Nb2 O7 occurred at 750°C via an intermediate perovskite phase of composition close to Sr0.82 NbO3 . The crystallization of Ca2 Nb2 O7 occurred at 600°C directly without any intermediate phases. Sintered Sr2 Nb2 O7 and Ca2 Nb2 O7 pellets showed a preferred grain orientation. Microstructural studies revealed an increase in grain growth and associated orientation with sintering temperature. 相似文献
136.
Summary Dense polymer membranes were made by mixing aqueous solutions of hydrophilic polymers poly(vinyl alcohol) (PVA) and polyethyleneimine
(PEI) in different ratios for investigating the separation of Tetrahydrofuran (THF)/water azeotropic mixtures by pervaporation
(PV). In order to gain a more detailed picture of the molecular transport phenomenon, we have performed sorption gravimetric
experiments at 30 °C to compute diffusion, swelling, sorption and permeability coefficients of PVA/PEI membranes in the presence
of THF and water. The membranes were found to have good potential for breaking the azeotrope of THF at 6% concentration of
water. An increase in PVA content in the blend caused a reduction in the flux and an increase in selectivity. Among the blends
tested in the study, the 5:1 PVA/PEI blend membrane showed the highest separation factor of 181.5, exhibited a flux of 1.28 kg/m2h for THF respectively at azeotropic feed composition. 相似文献
137.
Solé M Chatzinotas A Sridhar KR Harms H Krauss G 《The Science of the total environment》2008,406(1-2):324-330
Traditional methods used for studying communities of aquatic hyphomycetes are based on the detection and identification of their asexual spores under a microscope. These techniques limit detection to aquatic fungi present in sufficient quantity and capable of sporulating under laboratory conditions. Our objective was to develop a molecular approach to detect and monitor all types of fungi (i.e. strictly or facultatively aquatic) in harsh habitats (i.e. groundwater wells and heavily polluted surface water) where fungal biomass may become limited. We developed a semi-nested PCR protocol for fungal 18S ribosomal RNA genes coupled to subsequent analysis of the PCR products by Temperature Gradient Gel Electrophoresis (TGGE) to monitor the fungal community structure in aquatic habitats characterized by a pollution gradient. Our TGGE-protocol was compared with the traditional morphological approach and revealed a higher diversity in groundwaters and in some polluted surface waters. Thus, PCR-TGGE is a promising alternative in particular in habitats with low fungal biomass. The dynamics of fungal biomass and sporulation rates during the first weeks of leaf colonization showed that habitats with adverse ecological conditions allow only reduced fungal growth, which might subsequently impact upper trophic levels and thus interfere with key ecological processes of leaf decomposition. 相似文献
138.
139.
Crowley MM Zhang F Repka MA Thumma S Upadhye SB Battu SK McGinity JW Martin C 《Drug development and industrial pharmacy》2007,33(9):909-926
Interest in hot-melt extrusion techniques for pharmaceutical applications is growing rapidly with well over 100 papers published in the pharmaceutical scientific literature in the last 12 years. Hot-melt extrusion (HME) has been a widely applied technique in the plastics industry and has been demonstrated recently to be a viable method to prepare several types of dosage forms and drug delivery systems. Hot-melt extruded dosage forms are complex mixtures of active medicaments, functional excipients, and processing aids. HME also offers several advantages over traditional pharmaceutical processing techniques including the absence of solvents, few processing steps, continuous operation, and the possibility of the formation of solid dispersions and improved bioavailability. This article, Part I, reviews the pharmaceutical applications of hot-melt extrusion, including equipment, principles of operation, and process technology. The raw materials processed using this technique are also detailed and the physicochemical properties of the resultant dosage forms are described. Part II of this review will focus on various applications of HME in drug delivery such as granules, pellets, immediate and modified release tablets, transmucosal and transdermal systems, and implants. 相似文献
140.
Kapila D. Jain A. Nandakumar M. Ashburn S. Vasanth K. Sridhar S. 《Semiconductor Manufacturing, IEEE Transactions on》1999,12(4):457-461
The high energy retrograde well implants for sub-0.18 microns CMOS are done at a normal or near normal incidence to minimize the shadowing due to the thick photoresist edges. The endstation geometry in a high energy implanter results in an incident angle variation across the wafer, which causes strong spatial variations in the well profile and can negatively impact device performance. We show that the spatial variations can have significant impact on shallow trench isolation (STI), by causing in a deterministic pattern the failure of STI devices on a wafer. These spatial variations are important and need to be taken into consideration for STI design 相似文献