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Cludia Jassica Gonalves Moreno Henriqueta Monalisa Farias Rafael de Lima Medeiros Talita Katiane de Brito Pinto Johny Wysllas de Freitas Oliveira Francimar Lopes de Sousa Jr. Mayara Jane Campos de Medeiros Bruno Amorim-Carmo Gabriela Santos-Gomes Daniel de Lima Pontes Hugo Alexandre Oliveira Rocha Nilton Fereira Frazo Marcelo Sousa Silva 《International journal of molecular sciences》2022,23(15)
Leishmanolysin, also known as major promastigote protease (PSP) or gp63, is the most abundant surface glycoprotein of Leishmania spp., and has been extensively studied and recognized as the main parasite virulence factor. Characterized as a metalloprotease, gp63 can be powerfully inactivated in the presence of a metal chelator. In this study, we first used the structural parameters of a 7-hydroxycoumarin derivative, L1 compound, to evaluate the theoretical–computational experiments against gp63, comparing it with an available metal chelator already described. The methodology followed was (i) analysis of the three-dimensional structure of gp63 as well as its active site, and searching the literature and molecular databases for possible inhibitors; (ii) molecular docking simulations and investigation of the interactions in the generated protein–ligand complexes; and (iii) the individual energy of the gp63 amino acids that interacted most with the ligands of interest was quantified by ab initio calculations using Molecular Fraction with Conjugated Caps (MFCC). MFCC still allowed the final quantum balance calculations of the protein interaction to be obtained with each inhibitor candidate binder. L1 obtained the best energy quantum balance result with −2 eV, followed by DETC (−1.4 eV), doxycycline (−1.3 eV), and 4-terpineol (−0.6 eV), and showed evidence of covalent binding in the enzyme active site. In vitro experiments confirmed L1 as highly effective against L. amazonensis parasites. The compound also exhibited a low cytotoxicity profile against mammalian RAW and 3T3 cells lines, presenting a selective index of 149.19 and 380.64 µM, respectively. L1 induced promastigote forms’ death by necrosis and the ultrastructural analysis revealed disruption in membrane integrity. Furthermore, leakage of the contents and destruction of the parasite were confirmed by Spectroscopy Dispersion analysis. These results together suggested L1 has a potential effect against L. amazonensis, the etiologic agent of diffuse leishmaniasis, and the only one that currently does not have a satisfactory treatment. 相似文献
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A positive-sequence current baseddirectional relaying approach for CCVTsubsidence transient condition
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With the increasing connection of controllable devices to isolated community microgrid, an economic
operation model of isolated community microgrid based on the temperature regulation characteristics of
temperature controlling devices composed of wind turbine, micro-gas turbine, energy storage battery and
heat pump is proposed. With full consideration of various economic costs, including fuel cost, start-stop
cost, energy storage battery depletion expense and penalty for wind curtailment, the model is solved by
hybrid particle swarm optimization (HPSO) algorithm. The optimal output of the micro-sources and total
operating cost of the system in the scheduling cycle are also obtained. The case study demonstrates that
temperature adjustment of temperature controlling devices can adjust the power load indirectly and
increase the schedulability of the isolated community microgrid, and reduce the operating cost of the
microgrid. 相似文献