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排序方式: 共有627条查询结果,搜索用时 15 毫秒
41.
Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes 总被引:1,自引:0,他引:1
Matsumoto K Morimoto K Gohda A Shimada H Taguchi S 《Journal of Bioscience and Bioengineering》2011,111(4):485-488
Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold higher than the control transformants harboring the wild-type phaB gene. The immunodetection revealed an increased production of PhaB in leaves, indicating that the enhanced expression of PhaB was effective to increase P(3HB) production in tobacco. In contrast, codon-optimization of the phaC gene exhibited no apparent effect on P(3HB) production. This result suggests that the efficiency of PhaB-catalyzed reaction contributed to the flux toward P(3HB) biosynthesis in tobacco leaves. 相似文献
42.
Yoshitomi T. Saito M. Ohguro T. One M. Momose H.S. Morifuji E. Morimoto T. Katsumata Y. Iwai H. 《Electron Devices, IEEE Transactions on》1998,45(6):1295-1299
A silicided silicon-sidewall source and drain (S4D) structure is proposed for sub-0.1-μm devices. The merit of the S4D structure is that the series resistance of the source and drain is significantly reduced since the silicide layer is attached very close to the gate electrode and the silicon sidewall can be doped very highly. Thus, very high drain current drive can be expected, Another advantage of this structure is that the source and drain extensions are produced by the solid-phase diffusion of boron from the highly doped silicon-sidewall. Thus, shallow extensions with very high doping can be realized. A 75-nm gate length pMOSFET fabricated with this structure is shown to exhibit excellent electrical characteristics 相似文献
43.
Kawai S. Morimoto M. Mutoh N. Teranishi N. 《Electron Devices, IEEE Transactions on》1995,42(4):652-655
Photo response in CCD image sensors with Vertical-Overflow-Drain (VOD) was analyzed in an attempt to discover a way to lessen the photo response rise that accompanies increasing incident light intensity in the saturation region. A photo response analysis based on transistor I-V characteristics revealed that the extent of rise in the saturation region is uniquely determined by the non-ideality factor and temperature. Calculation of the non-ideality factor and its dependence on P-well impurity concentration and layer thickness further revealed that fabrication of P-wells with lower impurity concentrations and thicker layers would be effective in suppressing photo response rise 相似文献
44.
This paper proposes a novel method for color restoration that can effectively apply accurate color based on spectral information to a segmented image using the normalized cut technique. Using the proposed method, we can obtain a digital still camera image and spectral information in different environments. Also, it is not necessary to estimate reflectance spectra using a spectral database such as other methods. The synthesized images are accurate and high resolution. The proposed method effectively works in making digital archive contents. Some experimental results are demonstrated in this paper. 相似文献
45.
The performance of permanent magnet synchronous machines (PMSMs) has improved rapidly by the progress in elemental technologies such as electromagnetic material technology, computer‐aided design technology, control technique, and drive circuit technology, and thus PMSMs are attractive as high‐performance machines in various fields. This paper describes the recent technology and the trends in PMSMs. To begin with the PMSMs are classified by the ratio of magnet torque to the reluctance torque and their features described, then the trend of the motor design and the electromagnetic material for highly efficient PMSM is shown. The technologies that help to reduce the vibration and noise are also described, and the recently developed PMSMs for traction drive application are introduced. Copyright © 2007 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. 相似文献
46.
热压/热变形Nd2Fe14B/α-Fe纳米双相永磁体的研究 总被引:3,自引:0,他引:3
为了制备各向异性块状Nd2Fe14B/α—Fe纳米双相永磁体,研究了热压/热变形工艺参数与样品微观组织结构、磁性能之间的关系。结果表明,饱和磁化强度Js随模压温度的升高而提高;而剩磁Jr、内禀矫顽力Hcj和最大磁能积(BH)max开始都随模压温度的升高而上升,但超过一定温度后反而降低;同时提高热压压力会使磁性能增加,而热变形温度对磁性能影响很小。热变形后样品垂直于压力方向的磁性能略高于平行于压力方向,呈现出轻微的各向异性。Nd2Fe14B/α—Fe纳米双相永磁材料在热压/热变形后没有产生晶粒的择优长大,在晶体学上仍然是各向同性的。 相似文献
47.
Interannual variations in terrestrial carbon cycle over tropical rainforests affect the global carbon cycle. Terrestrial ecosystem models show the interannual relationship between climate changes due to El Niño‐Southern Oscillation (ENSO) and net primary production over tropical rainforests. However, we need an independent analysis using satellite‐based vegetation index and climate parameters. In the present study, we extracted the ENSO‐related interannual variations from time‐series in Normalized Difference Vegetation Index (NDVI) and climate data from 1981 to 2000, and analysed their relevance. We detected relationships among NDVI, ENSO, and climate parameters from long‐term data with negative NDVI–ENSO, NDVI–temperature, and positive NDVI–precipitation relations. These correlations suggest that interannual variability in vegetation activities over tropical rainforests could be extracted from NDVI time‐series despite noise components in NDVI data, and that interannual changes in precipitation and temperature caused by ENSO play a more important role in vegetation activities over tropical rainforests than in incoming surface solar radiation. 相似文献
48.
49.
Sato H. Wada T. Ohbayashi S. Kozaru K. Okamoto Y. Higashide Y. Shimizu T. Maki Y. Morimoto R. Otoi H. Koga T. Honda H. Taniguchi M. Arita Y. Shiomi T. 《Solid-State Circuits, IEEE Journal of》1999,34(11):1571-1579
This paper describes a 0.25-μm, 64 K×36 bit pipelined burst SRAM using a 6.156-μm2 cell. It realizes over 500-MHz operation using a lower cost double metal process, Internal 16 K×144 organization by T-shaped bit line array reduces 20% of latency, 20% of active power, and 8.5% of die size. The low power also enables us to use lower cost thin quad flat type packages. Our solution to the soft error problem, a shallow triple well structure and four-transistor cell with stacked capacitor, improved soft error rate by 3.5 orders of magnitude compared with the conventional SRAM cell 相似文献
50.
Sumio Chono Yoshihiko Tauchi Kazuhiro Morimoto 《Drug development and industrial pharmacy》2013,39(1):125-135
ABSTRACTIn order to confirm the efficacy of liposomes as a drug carrier for atherosclerotic therapy, the influence of particle size on the distribution of liposomes to atherosclerotic lesions in mice was investigated. In brief, liposomes of three different particle sizes (500, 200, and 70 nm) were prepared, and the uptake of liposomes by the macrophages and foam cells in vitro and the biodistributions of liposomes administered intravenously to atherogenic mice in vivo were examined. The uptake by the macrophages and foam cells increased with the increase in particle size. Although the elimination rate from the blood circulation and the hepatic and splenic distribution increased with the increase in particle size in atherogenic mice, the aortic distribution was independent of the particle size. The aortic distribution of 200 nm liposomes was the highest in comparison with the other sizes. Surprisingly, the aortic distribution of liposomes in vivo did not correspond with the uptake by macrophages and foam cells in vitro. These results suggest that there is an optimal size for the distribution of liposomes to atherosclerotic lesions. 相似文献