Characterization of microbial community structures and their activities in single anaerobic granules by beta imaging, microsensors and fluorescence in situ hybridization

The spatial distribution of microorganisms and their in situ activities in anaerobic granules were investigated by fluorescence in situ hybridization (FISH), beta imaging and microsensors. FISH results revealed a layered structure of microorganisms in the granule, where Chloroflexi was present in the outermost layer, Smithella spp. and Syntrophobacter spp. were found in a depth of ca. 100 μm, and Archaea was restricted to the inner layer (below ca. 300 μm from the surface). Substrate uptake patterns elucidated by beta imaging demonstrated that glucose uptake was highest at 50 μm depth, whereas propionate uptake had a peak at 200 μm depth. In addition, microsensor measurements revealed that acid was produced mainly at 100 μm depth and H(2) production was detected at a depth from 100 to 200 μm. H(2) consumption and corresponding CH(4) production were found below 200 μm from the surface. Direct comparison of these results implied sequential degradation of complex organic compounds in anaerobic granules; Chloroflexi contributed to fermentation of organic compounds and acid production in the outermost layer, volatile fatty acids were oxidized and H(2) was produced mainly by Smithella spp. and Syntrophobacter spp. at a depth from 100 to 200 μm, and Archaea produced CH(4) below ca. 300 μm from the surface.     

Case learning for CBR-based collision avoidance systems

With the rapid development of case-based reasoning (CBR) techniques, CBR has been widely applied to real-world applications such as collision avoidance systems. A successful CBR-based system relies on a high-quality case base, and a case creation technique for generating such a case base is highly required. In this paper, we propose an automated case learning method for CBR-based collision avoidance systems. Building on techniques from CBR and natural language processing, we developed a methodology for learning cases from maritime affair records. After giving an overview on the developed systems, we present the methodology and the experiments conducted in case creation and case evaluation. The experimental results demonstrated the usefulness and applicability of the case learning approach for generating cases from the historic maritime affair records.     

Capability and limitation of max- and sum-type construction of Lyapunov functions for networks of iISS systems

This paper addresses the problem of verifying stability of networks whose subsystems admit dissipation inequalities of integral input-to-state stability (iISS). We focus on two ways of constructing a Lyapunov function satisfying a dissipation inequality of a given network. Their difference from one another is elucidated from the viewpoint of formulation, relation, fundamental limitation and capability. One is referred to as the max-type construction resulting in a Lipschitz continuous Lyapunov function. The other is the sum-type construction resulting in a continuously differentiable Lyapunov function. This paper presents geometrical conditions under which the Lyapunov construction is possible for a network comprising $n\ge 2$ subsystems. Although the sum-type construction for general $n>2$ has not yet been reduced to a readily computable condition, we obtain a simple condition of iISS small gain in the case of $n=2$. It is demonstrated that the max-type construction fails to offer a Lyapunov function if the network contains subsystems which are not input-to-state stable (ISS).     

Colorimetric microdetermination of sulphites in foods by use of the modified rankine apparatus. IV.

Summary Detection and determination of traces of sulphites in foods was attempted by use of the modified Rankine apparatus and pararosaniline colorimetry. Replacement of alkaline titration reported previously by pararosaniline colorimetry lowered the absolute detection limit from 30 g (titration method) to 2 g. In view of clean analysis, in the color developing system, 0.1 N-sodium hydroxide was used in place of mercuric chloride solution commonly used as an absorbant of sulphites. In order to prevent oxidative decomposition of sulphites during operation, nitrogen gas was used as carrier instead of air. Dimedone and sodium azide were used for the elimination of aldehydes and nitrites, respecitvely, in the sample, which will disturb the color development of sulphites with pararosaniline-formaldehyde reagents. With this improved method, it was possible to determine the residual sulphites in frozen peeled shrimps, sugared beans and other foods with low sulphite contents accurately.

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Colorimetrische Mikrobestimmung von Sulfiten in Lebensmitteln bei Anwendung der modifizierten IV. Rankine Apparatur

Zusammenfassung Geringe Sulfitmengen in Lebensmitteln (geschälte Garnelen, gezuckerte Bohnen) können colorimetrisch bestimmt werden. Die neuentwickelte Methode beruht auf einer Kombination von colorimetrischer Bestimmung mittels p-Rosanilin und der Bestimmungsmethode nach Rankine. Auf diese Weise lassen sich Gehalte von 2 g noch genau bestimmen. Bei der Farbentwicklung wurde das giftige Quecksilbertetrachlorid durch 0.1 n-NaOH ersetzt, anstelle von Luft Stickstoff als Trägergas verwendet und somit eine Oxydation des Sulfits während der Bestimmung vermieden. Da Nitrit und Aldehyde die Farbentwicklung stören, wurde ihr Einfluß durch Dimedon und Natriumazid ausgeschaltet.

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Studies on the Analyses of Sulphites in Foods (IV)     

Electrical Inhomogeneity at the Mott Transition in the Band Width Controlled κ-(BEDT-TTF)2Cu[N(CN)2Br

The electronic phase separation on macroscopic scale is studied in the organic Mott system κ-(BEDT-TTF)₂Cu[N(CN)₂]Br by means of scanning microregion infrared spectroscopy using the synchrotron radiation. The phase separation appears in the vicinity of the Mott boundary in the single crystals of which the band width is controlled by partly substituting the BEDT-TTF molecule with the deuterated one. The transport properties under the phase separation are considered to be influenced by the percolation process of the domains.     

Preparation of Laminin-apatite-polymer Composites Using Metastable Calcium Phosphate Solutions

1Introduction Themostimportantrequirementofapercutaneous deviceisthepreventionofbacterialinfectionthroughtheinterfacebetweenthematerialandtheskin.Therefore,a percutaneousdevicehastoadherefirmlytoskintissueandpreventepidermaldowngrowth.Previously,Aokietalp…     

Construction of ULT-MRI cryostat for ultra low temperature physics

No Heading We have constructed an Ultra Low Temperature cryostat for Magnetic Resonance Imaging (ULT-MRI) applicable for various condensed phases of ³He at ultra low temperature, such as Nuclear Ordered Solid ³He or superfluid ³He. In contrast to conventional MRI for general use, where a pulsed-field gradient method is commonly used, we used a steady-field gradient method to avoid eddy current heating to metal parts at ultra low temperature. To avoid nonlinear response from the nuclear ordered spin system, which contradicts a principle of conventional MRI, we employed Free Induction Decay (FID) after a small tipping angle RF pulse to obtain the spectrum.PACS numbers: 07.20Mc, 67.57.Lm, 67.80.Jd, 76.60.Pc, 83.85.Fg     

Solving a Nonlinear Problem in Magneto-Rheological Fluids Using the Immersed Interface Method

Many applications lead to a nonlinear elliptic interface problem in which the discontinuous coefficient depends on the solution and the material properties. A finite difference method based on Cartesian grids and the maximum principle preserving immersed interface method is proposed for the nonlinear elliptic interface problems discussed in this paper. Numerical experiments against the exact solutions reveal that our method is nearly second order accurate in the infinity norm. The method is applied to study the magneto-rheological field-responsive fluids that contain iron particles. Numerical experiments are performed against the results from the literature.     

Avoiding Routing Loops on the Internet

Suppose that some particular link in the Internet is currently congested. A natural solution is to try to make packets bypass that link. This can be done by increasing the cost of that link intentionally, say from a ₁ to a ₂, since the Internet uses shortest-path routing. Unfortunately, however, this often causes temporary loops for packet traveling, called routing loops. In this paper we show that routing loops can be avoided by increasing the cost of the link not directly from a ₁ to a ₂ but through an intermediate value, a ₃, i.e., from a ₁ to a ₃ and then to a ₂. We may need several intermediate values. We show that in this case the greedy strategy, namely, raising the cost as much as possible in each step, is optimal.