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31.
Functional expression and synergistic cooperation of xylan‐degrading enzymes from Hypocrea orientalis and Aspergillus niger 下载免费PDF全文
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Yong Pan Chen Zhu Wen Bin Zeng Pei Fu Chi Chen Bao Ming Xu Zhong Feng Gao 《Chembiochem : a European journal of chemical biology》2021,22(24):3431-3436
Rapid and sensitive point-of-care testing (POCT) is an extremely critical mission in practical applications, especially for rigorous military medicine, home health care, and in the third world. Here, we report a visual POCT method for adenosine triphosphate (ATP) detection based on Taylor rising in the corner of quadratic geometries between two rod surfaces. We discuss the principle of Taylor rising, demonstrating that it is significantly influenced by contact angle, surface tension, and density of the sample, which are controlled by ATP-dependent rolling circle amplification (RCA). In the presence of ATP, RCA reaction effectively suppresses Taylor-rising behavior, due to the increased contact angle, density, and decreased surface tension. Without addition of ATP, untriggered RCA reaction is favorable for Taylor rising, resulting in a significant height. With this proposed method, visual sensitive detection of ATP without the aid of other instruments is realized with only a 5 μL droplet, which has good selectivity and a low detection limit (17 nM). Importantly, this visual method provides a promising POCT tool for user-friendly molecular diagnostics. 相似文献
34.
测向误差的减小一直以来都是无线电测向精度提高的一个难点,对于不同的测向体制所采用的减小测向误差的方法都不尽相同。本文主要介绍的是相关干涉仪测向体制的基本原理以及通过内插法的应用来减小测向误差的方法。 相似文献
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Shuang Shao Yong Liu Chun-Shui Xu Ying-Xuan Xu Bin Wu Xiao-Shu Zeng Xian-Feng Lu Xiang-Jie Yang 《金属学报(英文版)》2015,(1):7-14
The microstructure and mechanical properties of Mg–6Zn–1Y and Mg–6Zn–3Y(wt%) alloys under different cooling rates were investigated. The results show that the second dendrite arm spacing(SDAS) of Mg–6Zn–1Y and Mg–6Zn–3Y is reduced by 32 and 30% with increasing cooling rates(Rc) from 10.2 to 23 K/s, which can be predicted using a empirical model of SDAS=68 R 0:45:45cand SDAS=73 R 0c, respectively. The compressive strength of both alloys increases with increasing the cooling rate, which is attributed to the increase of volume fraction(Vf) of secondary phases under high cooling rate. The interaction of the cooling rate and component with SDAS has been theoretically analyzed using interdependence theory. 相似文献
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Yong Liu Kangzhuang Chen Fengbo Dong Shuai Peng Yongjie Cui Chenyu Zhang Keqing Han Muhuo Yu Hui Zhang 《Ceramics International》2018,44(9):10199-10203
In this paper, polyborosilazane precursor was synthesied from HMDZ, HSiCl3, BCl3 and CH3NH2 using a multistep method. By controlling the storage conditions, parts of the polyborosilazane fibers were hydrolyzed. FT-IR, NMR, XRD, TEM and monofilament tensile strength test were employed to study the effects of hydrolysis of precursor on the structures and properties of polymer-derived SiBN ceramic fibers. FT-IR and NMR results indicate that Si-N group in PBSZ reacts with H2O to form Si-O-Si group. After pyrolysis reaction at 1400℃, Si-O-Si group will finally transformed into highly ordered cristobalite and β-quartz, resulting in formation of the wrinkled surface of the obtained SiBN ceramic fiber. The strip-like defects on fiber surface, according to monofilament tensile strength test, had a significant effect on mechanical property of the obtained SiBN ceramic fiber and caused no increase in fiber tensile strength of hydrolytic polyborosilazane fiber before and after pyrolytic process. 相似文献
39.
摘要:为了研究退火温度对镀锌DP980+Z烘烤硬化值的影响,退火温度控制在760~820℃之间,系统分析退火温度对烘烤硬化值的影响。通过准静态拉伸试验机测量烘烤硬化值及抗拉强度,采用lepara试剂对组织中的马氏体进行着色,利用金相显微镜及图像处理软件测量马氏体的体积分数;采用扫描电镜观察DP980+Z的双相组织特点,并且将组织图片通过CAD转化成有限元图进行网格划分,建立代表性体积单元(RVE),通过有限元分析铁素体、马氏体强度对烘烤硬化值的影响。在同样的变形量情况下,DP980+Z的原始屈服强度越高,烘烤硬化值越高。 相似文献
40.
WENXI GAO QIANQIAN MA CHENYU TANG YUELI ZHAN YINONG DUAN HUIHUA NI YUNZHAO XU 《Biocell》2020,44(4):597-605
Cervical cancer (CESC) is one of the most common cancers and affects the female genital tract. Consistent HPV
infection status has been determined to be a vital cause of tumorigenesis. HPV infection may induce changes to the
immune system and limit the host’s immune response. Immunotherapy is therefore essential to improving the overall
survival of both locally advanced and recurrent CESC patients. Using 304 relevant samples from TCGA, we assessed
immune cell function in CESC patients to better understand the status of both tumor micro-environment cells and
immune cells in CESC. Functional enrichment analysis, pathway enrichment analysis, and PPI network construction
were performed to explore the differentially expressed genes (DEGs). The analysis identified 425 DEGs, which
included 295 up-regulated genes and 130 down-regulated genes. We established that upregulation of CCL5 was
correlated with significantly better survival, meaning that CCL5 expression could serve as a novel prognostic
biomarker for CESC patients. We further focused on CCL5 as a hub gene in CESC, as it had significant correlations
with increased numbers of several types of immune cells. Cell-type fractions of M1 macrophages were significantly
higher in the high-immune-scores group, which was associated with better overall survival. Finally, we concluded that
CCL5 is a promising prognostic biomarker for CESC, as well as a novel chemotherapeutic target. 相似文献