Potential of bacteriocinogenic Lactococcus lactis subsp. lactis inhabiting low pH vegetables to produce nisin variants

Antimicrobial behavior of lactic acid bacteria (LAB) has been explored since many years to assess their ability to produce bacteriocin, a natural preservative, to increase the shelf life of food. This study aims to characterize bacteriocin producing strains of lactic acid bacteria isolated from acidic to slightly acidic raw vegetables including tomato, bell pepper and green chili and to investigate their potential to inhibit food related bacteria. Among twenty nine LAB screened for antimicrobial activity, three exhibited antagonism against closely related bacterial isolates which was influenced by varying temperature and pH. They were identified up to strain level as Lactococcus lactis subsp. lactis TI-4, L. lactis subsp. lactis CE-2 and L. lactis subsp. lactis PI-2 based on 16S rRNA gene sequence. Their spectrum of inhibition was observed against food associated strains of Bacillus subtilis and Staphylococcus aureus. Moreover, L. lactis subsp. lactis PI-2 selected on the basis of higher antimicrobial activity was further evaluated for bacteriocin production which was detected as nisin A and nisin Z. These findings suggest the possible use of L. lactis strains of vegetable origin as protective cultures in slightly acidic as well as slightly alkaline food by the bio-preservative action of bacteriocins.     

Seismic Behavior of As-Built, ACI-Complying, and CFRP-Repaired Exterior RC Beam-Column Joints

In this paper, the efficiency and effectiveness of carbon-fiber-reinforced polymer (CFRP) sheets for upgrading the shear strength and ductility of a seismically deficient exterior beam-column joint were studied and compared with an American Concrete Institute (ACI)-based design joint specimen. One as-built joint specimen, representing the preseismic code design and construction practice for joints and one ACI-based design joint specimen, satisfying the seismic design requirements of the current code of practice were cast. The as-built specimen was used as baseline (control) specimen. These two specimens (i.e., the as-built control and the ACI-based specimens) were subjected to cyclic lateral load histories to induce damage equivalent to damage expected from a severe earthquake. The damaged control specimen was then repaired by filling its cracks with epoxy and externally bonding CFRP sheets to the joint, the beam, and part of the column regions. This specimen was identified as the repaired specimen. The repaired specimen was subjected to a similar cyclic lateral load history, and its response history was recorded. The response histories of the as-built control, the repaired, and the ACI-based design specimen were then compared. The test results demonstrated that externally bonded CFRP sheets can effectively improve both the shear strength and the deformation capacity of seismically deficient and damaged beam-column joints to a state comparable to the ACI-based design joint.     

Studies to analyse the relationship between IFNα2b gene dosage and its expression,using a Pichia pastoris‐based expression system

Human interferon α2b (hIFNα2b) is the most important member of the interferon family. Escherichia coli, yeasts, mammalian cell cultures and baculovirus‐infected insect cells have been used for expressing recombinant human interferon. Recently a Pichia pastoris‐based expression system has emerged as an attractive system for producing functional human recombinant IFNα2b. In this regard, gene dosage is considered an important factor in obtaining the optimum expression of recombinant protein, which may vary from one protein to another. In the present study we have shown the effect of IFNα2b gene dosage on extracellular expression of IFNα2b recombinant protein from P. pastoris. Constructs containing from one to five repeats of IFNα2b‐expressing cassettes were created via an in vitro multimerization approach. P. pastoris host strain X‐33 was transformed using these expression cassettes. Groups of P. pastoris clones transformed with different copies of the IFNα2b expression cassette were screened for intrachromosomal integration. The IFNα2b expression level of stable transformants was checked. The copy number of integrated IFNα2b was determined by performing qPCR of genomic DNA of recombinant P. patoris clones. It was observed that an increase in copy number generally had a positive effect on the expression level of IFNα2b protein. Regarding the performance of multicopy strains, those obtained from transformation of multicopy vectors showed relatively high expression, compared to those generated using transformation vector having only one copy of IFNα2b. It was also observed that an increase in drug resistance of a clone did not guarantee its high expression, as integration of a marker gene did not always correlate with integration of the gene of interest. Copyright © 2013 John Wiley & Sons, Ltd.     

A copper based metal-organic framework as single source for the synthesis of electrode materials for high-performance supercapacitors and glucose sensing applications

The article describes the conversion of MOF-199 to Cu–Cu₂O–CuO/C 700 (1) and Cu–Cu₂O–CuO/C 800 (2) nanostructures by simple pyrolysis at 700 and 800 °C under inert atmosphere. The X-ray photoelectron spectroscopy analysis reveals that the nanostructures Cu–Cu₂O–CuO/C consist of graphitic carbon functionalized with carboxylic, carbonyl and hydroxyl functional groups with copper/copper oxide particles on surfaces. The electrochemical properties of 1 and 2 are evaluated as electrode material for supercapacitors using cyclic voltammetry, galvanostatic charge/discharge and electrochemical impedance spectroscopy. The results for the capacitive performance from cyclic voltammetry and galvanostatic charge/discharge reveal that both the samples have gravimetric capacitance greater than 750 F g⁻¹ at a scan rate of 2 mV s⁻¹ and current density of 2 mA cm⁻². The samples retain about 43% of their initial capacitance even at high scan rate of 75 mV s⁻¹. The cycling performance of the nanostructures illustrate that there is 5.5% capacitance loss after 3000 cycles. The sample 1 and 2 are washed with 1 mol L⁻¹ HCl solution to obtain copper oxide free materials Cu/C 700 (3) and Cu/C 800 (4). Samples 3 and 4 are tested as electrocatalysts for glucose sensing and the cyclic voltammetry measurement shows enhanced current densities compared to the literature values.     

Substrate Selection Influences Molecular Recognition in a Screen for Lymphoid Tyrosine Phosphatase Inhibitors

Assay design is an important variable that influences the outcome of an inhibitor screen. Here, we have investigated the hypothesis that protein tyrosine phosphatase inhibitors with improved biological activity could be identified from a screen by using a biologically relevant peptide substrate, rather than traditional phosphotyrosine mimetic substrates. A 2000‐member library of drugs and drug‐like compounds was screened for inhibitors of lymphoid tyrosine phosphatase (LYP) by using both a peptide substrate (Ac‐ARLIEDNE‐pCAP‐TAREG‐NH₂, peptide 1) and a small‐molecule phosphotyrosine mimetic substrate (difluoromethyl umbelliferyl phosphate, DiFMUP). The results demonstrate that compounds that inhibited enzyme activity on the peptide substrate had greater biological activity than compounds that only inhibited enzyme activity on DiFMUP. Finally, epigallocatechin‐3,5‐digallate was identified as the most potent inhibitor of lymphoid tyrosine phosphatase activity to date, with an IC₅₀ of 50 nM and significant activity in T‐cells. Molecular docking simulations provided a first model for binding of this potent inhibitor to LYP; this will constitute the platform for ongoing lead optimization efforts.     

Thiuram Disulfides as Pseudo‐irreversible Inhibitors of Lymphoid Tyrosine Phosphatase

We screened a small library of thiuram disulfides for inhibition of lymphoid tyrosine phosphatase (LYP) activity. The parent thiuram disulfide, disulfiram, inhibited LYP activity in vitro and in Jurkat T cells, whereas diethyldithiocarbamate failed to inhibit LYP at the concentrations tested. Compound 13 , an N‐(2‐thioxothiazolidin‐4‐one) analogue, was found to be the most potent LYP inhibitor in this series, with an IC₅₀ value of 3 μM . Compound 13 inhibits LYP pseudo‐irreversibly, as evidenced by the time‐dependence of inhibition, with a K_i value of 1.1 μM and a k_inact value of 0.004 s^?1. The inhibition of LYP by compound 13 could not be reversed significantly by incubation with glutathione or by prolonged dialysis, but could be partially reversed by incubation with dithiothreitol. Compound 13 also inhibited LYP activity in Jurkat T cells.     

MHD rotating flow of a third-grade fluid on an oscillating porous plate

Summary The rotating flow of a third-grade fluid on an oscillating porous plate in the presence of a transverse magnetic field is considered. An analytic solution of the governing nonlinear boundary layer equation is obtained. Expressions for the velocity profile are established. It is found that an external magnetic field has the same effect on the flow as the material parameters of the fluid. Further the symmetric and asymmetric nature of the solutions is discussed.     

Design and implementation of a planar helix for traveling wave tubes based on package compatible ball grid array technology

In this article, a novel planar helix traveling wave tube structure is proposed for S/X-band amplification. The planar helix structure is developed between two printed circuit boards such that they utilize solder balls to connect each other. This technique which comes originally from microelecronic packaging technology called ball grid array. To evaluate the performance of the proposed structure, one period of its geometry is modeled and the cold test parameters are calculated in CST microwave studio^TM. The fundamental space harmonic mode overlaps well with the beam-line in the dispersion diagram for both bands. The in- and out-coupler ports are also designed in the form of coplanar waveguides having good matching. For 150 periods of the planar helix, the 15 dB gain is obtained in particle-in-cell simulations for both bands. Finally, the fabrication process is implemented and the cold test is done.

     

The effects of MHD and buoyancy on Hematite water-based fluid past a convectively heated stretching sheet

Neural Computing and Applications - In the present paper, we examined the buoyancy effects on MHD two-dimensional boundary layer flow in the presence of heat transfer of Hematite–water...     

Synthesis and EMI shielding studies of polyaniline grafted conducting nanohybrid

Polyaniline (PANI) grafted conducting nanohybrids are successfully synthesized by emulsion graft polymerization. Maximum grafting of 644% is obtained on sepiolite at optimized concentrations and reaction conditions. The Fourier transform infrared (FT-IR) spectra of sepiolite grafted polyaniline (MS-g-PANI) nanohybrid revealed the presence of stretching vibrations of C=C bonds of polyaniline at 1580 and 1490 cm⁻¹ along with the characteristic peaks of sepiolite. Increased thermal stability of the nanohybrid is observed with the increase in polyaniline grafting. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) are used to study the structural changes caused by grafting. A progressive increase in electrical conductivity is observed with the increase in grafting yield. Maximum electrical conductivity of 0.427 Scm⁻¹ is observed in the nanohybrid having 644% grafting. 24.2 dB (>99% attenuation) EMI shielding effectiveness has been exhibited by the developed nanohybrids in X-band frequency range.