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81.
I Kohno H Iwasaki M Okutani Y Mochizuki S Sano Y Satoh T Ishihara H Ishii S Mukaiyama H Ijiri S Komori K Tamura 《Canadian Metallurgical Quarterly》1997,14(1):71-84
Cells of the central nervous system (CNS) normally do not express detectable levels of major histocompatibility complex (MHC) Class I antigens. However, MHC Class I expression can be induced after virus infection. We tested the hypothesis that virus-induced Class I expression is mediated by lymphocytes or cytokines using lymphocyte- and cytokine-deficient mice. We used Theiler's murine encephalomyelitis virus (TMEV), which induces CNS demyelination that maps genetically to the D region of MHC Class I and is associated with high levels of Class I products. TMEV infection of severe combined immunodeficiency (SCID) and recombination activation gene-1-deficient mice, which lack B and T lymphocytes, resulted in equivalent H-2D and H-2K expression in brain and spinal cord, according to analysis of the area and intensity of immunoperoxidase staining. Class I antigens were demonstrated as early as 6 hours after infection, and they were more widely distributed than viral RNA, indicating that expression was induced indirectly via a soluble factor. To determine whether cytokines induced the expression, we infected mice lacking receptors for interferon-alpha/beta (IFN-alpha/beta R (-/-)), interferon-gamma (IFN-gamma R(-/-)), and tumor necrosis factor-alpha (TNFRp55(-/-)). TMEV-infected IFN-gamma R(-/-) and TN-FRp55(-/-) mice expressed Class I antigens in the CNS, whereas IFN-alpha/beta R(-/-) mice did not, establishing that IFN-alpha/beta mediated the expression. In contrast to the equivalent expression in SCID mice, we observed greater area and higher intensity of H-2D versus H-2K antigens in infected SCID mice reconstituted with normal spleen cells. Collectively, the data indicate that after TMEV infection, early generalized MHC Class I expression is mediated by IFN-alpha/beta independently of lymphocytes, but the differential regulation of H-2D over H-2K may be controlled by B and/or T lymphocytes. 相似文献
82.
We have characterized the binary LB films of merocyanine dye (MS) and arachidic acid (C20) before and after hydrothermal treatment (HTT), which is defined as a heat treatment under relative humidity of 100%, focusing on the morphology studied by bright field (BF) microscopy and fluorescence (FL) microscopy. BF microscopy observation has revealed that the as-deposited MS-C20 binary LB film is found to emit intense red fluorescence over the whole film area by 540-nm excitation. Since the surface image is almost featureless, it is considered that the crystallite sizes of J-aggregate are less than 10 μm. Interestingly, after HTT, round-shaped domains are observed in the LB systems, and the sizes are reaching 100 μm in diameter. Crystallites of J-aggregate, which are bluish in color and emit intense red fluorescence, tend to be in the round domains. We have observed two different types of domains, i.e., blue-rimmed domains and white-rimmed domains, which are postulated to be confined in the inner layers and located at the outermost layer, respectively. The thickness of the domains is equal to or less than that of the double layer of the MS-C20 mixed LB film, which is ca. 5.52 nm. The molecular order of MS in the J-aggregate is improved by the HTT process leading to the significant sharpening of the band shape together with the further red shift of the band (from 590 to 594 nm up to 597 to 599 nm). The reorganized J-band is considered to be ‘apparently’ isotropic owing to the random growth of the J-aggregate in the film plane. We consider that the lubrication effect by the presence of water molecules predominates in the HTT process. 相似文献
83.
Many studies have been conducted on the evacuation behavior on the staircases of buildings, but very little data are available for a situation with many occupants in a crowded high‐rise building. Therefore, this study investigated the evacuation behavior of a large number of evacuees on the staircase of a 25‐story high‐rise building. A total evacuation drill was conducted with 2088 evacuees, and the behavior of 1136 evacuees on the landings of the south staircase was recorded by a video recorder on the ceiling. The relationship between the density and speed of the evacuees on the landings was analyzed from the evacuation data for two situations: without and with merging in the stair flow. The evacuation stair flow in this drill had merging occupants entering from the floors, but no one entered from the lower floors during the latter period of the drill. Therefore, the flow during the latter period was treated as non‐merging flow, for which it was observed that, when the staircase was fully crowded, the density on the landings in the moving situation was different from that in the stopped situation. Moreover, the density on the landings was different from that on the treads. Furthermore, in the merging flow, a merging ratio of approximately 50:50 occurred during the congested evacuation. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
84.
85.
Heme can be removed from a number of native hemoproteins, thus forming corresponding apoproteins, each of which provides a site for binding of a metal complex. In one example, myoglobin, an O2 storage protein, can be reconstituted with iron porphycene to dramatically enhance the O2 affinity. Although it is known that myoglobin has poor enzymatic activity, the insertion of iron corrole or iron porphycene into apomyoglobin increases its H2O2-dependent peroxidase/peroxygenase activities. Furthermore, reconstitution with manganese porphycene promotes hydroxylation of an inert C H bond. It is also of interest to insert a non-porphyrinoid complex into an apoprotein. A cavity of apocytochrome c has been found to bind a diiron carbonyl complex, serving as a functional model of diiron hydrogenase. Aponitrobindin has a rigid β-barrel structure that provides an excellent cavity for covalently anchoring a metal complex. A rhodium complex embedded in the cavity of genetically modified nitrobindin has been found to promote stereoselective polymerization of phenylacetylene. 相似文献
86.
87.
Enhancing effect of monoolein surfactant on carbon nanoparticle synthesis by arc discharge in liquid
Tawatchai Charinpanitkul Noriaki Sano Poonlasak Muthakarn 《Materials Research Bulletin》2009,44(2):324-917
Effect of monoolein surfactant which promotes n-hexane to dissolve in water was investigated to enhance the carbon nanoparticle (CNP) synthesis by arc discharge in liquid. The production rate of CNPs consisting of multi-shelled fullerenes and multi-walled carbon nanotubes was increased by the addition of the n-hexane to water with support of monoolein surfactant. An optimal amount of monoolein led to the maximal production rate of CNPs which was ca. two-fold higher than that obtained from a pure water system. This effect is ascribed to the role of n-hexane and monoolein to provide carbon clusters to the arc reactive zone. The hydrodynamic particle sizes of CNPs and their crystallinity were also enhanced by the addition of these organic compounds. As the vapor pressure of the organic component is significantly suppressed, the proposed method is much safer than that using pure organic liquid. 相似文献
88.
Abstract— By applying an energy‐recovery method to their driver circuits, the circuit power consumption of plasma displays can be reduced. However, further power reduction is necessary for large‐sized higher‐resolution displays such as used for Super HDTV. The ideal adiabatic charge method has been proven to be able to minimize circuit resistive element power consumption to 81 % of the conventional energy‐recovery‐circuit resistive‐element loss. An experimental pseudo‐adiabatic charge circuit that reduces the power consumption to about 90% of a conventional circuit has been demonstrated. The power consumption caused by both the resistance loss and the discharge loss of the switching‐element parasitic capacitances was analyzed. 相似文献
89.
Kitsakorn Locharoenrat Haruyuki Sano 《Science and Technology of Advanced Materials》2013,14(4):277-281
We report on the experimental observation of surface plasmon resonance in Cu nanowires fabricated by shadow deposition method. When the incident light is polarized perpendicular to the wire axes, plasmon maxima appeared at about 2.3 eV in the absorption spectra. Plasmon resonance appeared at lower photon energy when the incident light is polarized parallel to the wire axes. Resonance peaks move to lower energy when the nanowire widths are increased. We have found that finite-difference time-domain (FDTD) simulation gives better results than Maxwell–Garnett model in explaining the relation between the light polarization and the energies of the observed absorption maxima. 相似文献
90.