Optimization of the Active Composition of the Wind Farm Using Genetic Algorithms

The article presents the results of research on the possibilities of using genetic algorithms for solving the multicriteria optimization problem of determining the active components of a wind farm. Optimization is carried out on two parameters: efficiency factor of wind farm use (integrated parameter calculated on the basis of 6 parameters of each of the wind farm), average power deviation level (average difference between the load power and energy generation capabilities of the active wind farm). That was done an analysis of publications on the use of genetic algorithms to solve multicriteria optimization problems. Computer simulations were performed, which allowed us to analyze the obtained statistical data and determine the main optimization indicators. That was carried out a comparative analysis of the obtained results with other methods, such as the dynamic programming method; the dynamic programming method with the general increase of the set loading; the modified dynamic programming method, neural networks. It is established that the average power deviation for the genetic algorithm and for the modified dynamic programming method is located at the same level, 33.7 and 28.8 kW, respectively. The average value of the efficiency coefficient of wind turbine used for the genetic algorithm is 2.4% less than for the modified dynamic programming method. However, the time of finding the solution by the genetic algorithm is 3.6 times less than for the modified dynamic programming method. The obtained results provide an opportunity to implement an effective decision support system in energy flow management.     

Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.     

An adaptive learning algorithm for a wavelet neural network

Abstract: An optimal online learning algorithm of a wavelet neural network is proposed. The algorithm provides not only the tuning of synaptic weights in real time, but also the tuning of dilation and translation factors of daughter wavelets. The algorithm has both tracking and smoothing properties, so the wavelet networks trained with this algorithm can be efficiently used for prediction, filtering, compression and classification of various non-stationary noisy signals.     

Sensitive Immunofluorescent Detection of the PRAME Antigen Using a Practical Antibody Conjugation Approach

Bioconjugation of antibodies with various payloads has diverse applications across various fields, including drug delivery and targeted imaging techniques. Fluorescent immunoconjugates provide a promising tool for cancer diagnostics due to their high brightness, specificity, stability and target affinity. Fluorescent antibodies are widely used in flow cytometry for fast and sensitive identification and collection of cells expressing the target surface antigen. Nonetheless, current approaches to fluorescent labeling of antibodies most often use random modification, along with a few rather sophisticated site-specific techniques. The aim of our work was to develop a procedure for fluorescent labeling of immunoglobulin G via periodate oxidation of antibody glycans, followed by oxime ligation with fluorescent oxyamines. Here, we report a novel technique based on an in situ oxime ligation of ethoxyethylidene-protected aminooxy compounds with oxidized antibody glycans. The approach is suitable for easy modification of any immunoglobulin G, while ensuring that antigen-binding domains remain intact, thus revealing various possibilities for fluorescent probe design. The technique was used to label an antibody to PRAME, a cancer-testis protein overexpressed in a number of cancers. A 6H8 monoclonal antibody to the PRAME protein was directly modified with protected-oxyamine derivatives of fluorescein-type dyes (FAM, Alexa488, BDP-FL); the stoichiometry of the resulting conjugates was characterized spectroscopically. The immunofluorescent conjugates obtained were applied to the analysis of bone marrow samples from patients with oncohematological diseases and demonstrated high efficiency in flow cytometry quantification. The approach can be applied for the development of various immunofluorescent probes for detection of diagnostic and prognostic markers, which can be useful in anticancer therapy.     

Stabilizers for peroxydicarbonate initiator solutions

Liquid dialkyl peroxydicarbonates are used as initiators in the PVC industry. Because of the thermal reactivity of these initiators, they require very low temperature storage, shipment, and handling. At temperatures above 10°C, most undergo autoaccelerated self-induced decomposition. In other words, their self-accelerating decomposition temperature (SADT) is exceeded. New additives have been discovered which increase the SADT of the initiators. These additives effectively stabilize the products, making them safer to handle, store, and ship. The proprietary additives and a mechanism of stabilization are discussed. We also include a section concerning the implications these products have for future initiator formulation.     

Effects of Oleic Acid and Phospholipids on the Formation of Lecithin Organogel and Microemulsion

The formation of organogels and microemulsions of lecithin in the presence of a biocompatible cosurfactant, oleic acid, was studied. Low content of oleic acid ([oleic acid]/[lecithin] < 0.1) in the lecithin–oleic acid–dodecane–water system leads to an expansion of the region of existence and to a decrease in the viscosity of lecithin organogels. At high contents of oleic acid ([oleic acid]/[lecithin] > 0.6), low‐viscosity microemulsion exists in the system. Phosphatidylethanolamine, lysophosphatidylcholine, and other phospholipids that are present as impurities in the commercial samples of soybean lecithin can act as cosurfactants. For the first time, the formation of lecithin organogels in the systems containing commercial samples of soybean lecithin with phosphatidylcholine concentrations of 69.3 wt% (Lipoid S75) and 52.9 wt% (Lipoid S45) and saturated aliphatic hydrocarbons is demonstrated. The gelation is observed at T =25 °C in octane, decane, dodecane, and hexadecane for Lipoid S75 and in dodecane and hexadecane for Lipoid S45. A decrease in the degree of purification of lecithin leads to an expansion of the regions of existence of the organogels and to a reduction of their viscosity.     

Comparison of some characteristics of thin‐film‐electroluminescent ZnS: Er,F planar and edge emitters

Abstract— The following main differences have been revealed in the characteristics of an edge thin‐film‐electroluminescent ZnS: Er,F emitter compared to those of a similar planar emitter: (1) the 1.535‐μm band more highly dominates over other bands in the EL spectrum; (2) the voltage (V) dependence of the intensity of this band is the strongest; (3) the 1.535‐μm band narrows with increasing voltage and its frequency. The above differences are explained, firstly, by smaller optical losses in the ZnS: Er,F film for the near‐infrared emission than for the visible one and, secondly, by an optical amplification over the 1.535‐μm band in the edge emitter.     

Flow through regular polygonal channels

A general method for analyzing viscous flow through regular polygonal channels is presented, based upon the assumption that the flow rate through regular polygonal sections equals that through circular sections of the same area. In addition we offer alternatives which apply specifically to equilaterial triangles and to squares.