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Shiming Zhang Yihang Chen Hao Liu Zitong Wang Haonan Ling Changsheng Wang Jiahua Ni Betul Celebi Saltik Xiaochen Wang Xiang Meng Han-Jun Kim Avijit Baidya Samad Ahadian Nureddin Ashammakhi Mehmet R. Dokmeci Jadranka Travas-Sejdic Ali Khademhosseini 《Advanced materials (Deerfield Beach, Fla.)》2020,32(1):2070005
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Rohollah Nasiri Amir Shamloo Samad Ahadian Leyla Amirifar Javad Akbari Marcus J. Goudie KangJu Lee Nureddin Ashammakhi Mehmet R. Dokmeci Dino Di Carlo Ali Khademhosseini 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(29)
Cell separation is a key step in many biomedical research areas including biotechnology, cancer research, regenerative medicine, and drug discovery. While conventional cell sorting approaches have led to high‐efficiency sorting by exploiting the cell's specific properties, microfluidics has shown great promise in cell separation by exploiting different physical principles and using different properties of the cells. In particular, label‐free cell separation techniques are highly recommended to minimize cell damage and avoid costly and labor‐intensive steps of labeling molecular signatures of cells. In general, microfluidic‐based cell sorting approaches can separate cells using “intrinsic” (e.g., fluid dynamic forces) versus “extrinsic” external forces (e.g., magnetic, electric field, etc.) and by using different properties of cells including size, density, deformability, shape, as well as electrical, magnetic, and compressibility/acoustic properties to select target cells from a heterogeneous cell population. In this work, principles and applications of the most commonly used label‐free microfluidic‐based cell separation methods are described. In particular, applications of microfluidic methods for the separation of circulating tumor cells, blood cells, immune cells, stem cells, and other biological cells are summarized. Computational approaches complementing such microfluidic methods are also explained. Finally, challenges and perspectives to further develop microfluidic‐based cell separation methods are discussed. 相似文献
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Polyaniline and poly(aniline-co-o-anisidine) films were deposited on brass (Cu40Zn). The synthesis processes of homo and copolymer film were carried out under cyclic voltammetric condition from 0.12 M aniline and 0.06 M aniline + 0.06 M o-anisidine containing 0.2 M sodium oxalate solutions. Homo and copolymer films were characterized by scanning electron microscopy (SEM). SEM images clearly show that one of the brass electrodes was covered with a black copolymer film of strongly adherent homogeneous characteristic while the other one with a porous dark green homo polymer one. The corrosion performances of coated and uncoated electrodes in 3.5% NaCl were evaluated with the help of AC impedance spectroscopy, anodic polarization plots and open circuit potential–time curves. The protective effect of homo and copolymer films formed on brass grew in parallel with extended exposure time. It was only observed with copolymer-coated electrode that changes in the charge transfer resistance of copolymer-coated electrode were related to strong adsorption of copolymer film on the brass surface which led to the formation of a protective oxide layer due to its catalytic behaviour. 相似文献
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Polyaniline (PANI), poly(o‐anisidine), and poly[aniline‐co‐(o‐anisidine)] were synthesized by chemical oxidative polymerization with ammonium persulfate as an oxidizing reagent in an HCl medium. The viscosities, electrical conductivity, and crystallinity of the resulting polymers (self‐doped forms) were compared with those of the doped and undoped forms. The self‐doped, doped, and undoped forms of these polymers were characterized with infrared spectroscopy, ultraviolet–visible spectroscopy, and a four‐point‐probe conductivity method. X‐ray diffraction characterization revealed the crystalline nature of the polymers. The observed decrease in the conductivity of the copolymer and poly(o‐anisidine) with respect to PANI was attributed to the incorporation of the methoxy moieties into the PANI chain. The homopolymers attained conductivity in the range of 3.97 × 10?3 to 7.8 S/cm after doping with HCl. The conductivity of the undoped forms of the poly[aniline‐co‐(o‐anisidine)] and poly(o‐anisidine) was observed to be lower than 10?5 J/S cm?1. The conductivity of the studied polymer forms decreased by the doping process in the following order: self‐doped → doped → undoped. The conductivity of the studied polymers decreased by the monomer species in the following order: PANI → poly[aniline‐co‐(o‐anisidine)] → poly(o‐anisidine). All the polymer samples were largely amorphous, but with the attachment of the pendant groups of anisidine to the polymer system, the crystallinity region increased. The undoped form of poly[aniline‐co‐(o‐anisidine)] had good solubility in common organic solvents, whereas doped poly[aniline‐co‐(o‐anisidine)] was moderately crystalline and exhibited higher conductivity than the anisidine homopolymer. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci, 2006 相似文献