Peroxiredoxin 2 as a chemotherapy responsiveness biomarker candidate in osteosarcoma revealed by proteomics

Purpose : We aimed to identify novel chemotherapy responsiveness biomarkers for osteosarcoma (OS) by investigating the global protein expression profile of 12 biopsy samples from OS patients. Experimental design : Six patients were classified as good responders and six as poor responders, according to the Huvos grading system. The protein expression profiles obtained by 2‐D DIGE consisted of 2250 protein spots. Results : Among them, we identified 55 protein spots whose intensity was significantly different (Bonferroni adjusted p‐value<0.01) between the two patient groups. Mass spectrometric protein identification demonstrated that the 55 spots corresponded to 38 distinct gene products including peroxiredoxin 2 (PRDX 2). Use of a specific antibody against PRDX 2 confirmed the differential expression of PRDX 2 between good and poor responders, while PRDX 2 levels as measured by Western blotting correlated highly with their corresponding 2‐D DIGE values. The predictive value of PRDX 2 expression was further confirmed by examining an additional four OS cases using Western blotting. Conclusions and clinical relevance : These results establish PRDX 2 as a candidate for chemotherapy responsiveness marker in OS. Measuring PRDX 2 in biopsy samples before treatment may contribute to more effective management of OS.     

Proteomic profile of differentially expressed plasma proteins from dystrophic mice and following suberoylanilide hydroxamic acid treatment

Purpose: Histone Deacetylase Inhibitors (DI) ameliorates dystrophic muscle regeneration restoring muscular strength in the mdx mouse model of Duchenne muscular dystrophy (DMD). The further development of these compounds as drugs for DMD treatment is currently hampered by the lack of knowledge about DIs effect in large dystrophic animal models and that of suitable biomarkers to monitor their efficacy. Experimental design: In this study we applied proteomic analysis to identify differentially expressed proteins present in plasma samples from mdx mice treated with the Suberoylanilide hydroxamic acid (SAHA) and relative normal controls (WT). Results: Several differentially expressed proteins were identified between untreated wild type and mdx mice. Among these, fibrinogen, epidermal growth factor 2 receptor, major urinary protein and glutathione peroxidase 3 (GPX3) were constitutively up‐regulated in mdx, while complement C3, complement C6, gelsolin, leukaemia inhibitory factor receptor (LIFr), and alpha 2 macroglobulin were down‐regulated compared to WT mice. SAHA determined the normalization of LIFr and GPX3 protein level while apoliprotein E was de novo up‐regulated in comparison to vehicle‐treated mdx mice. Conclusions and clinical relevance: Collectively, these data unravel potential serological disease biomarkers of mdx that could be useful to monitor muscular dystrophy response to DI treatment.     

Differential regulation of redox proteins and chaperones in HbEβ-thalassemia erythrocyte proteome

Purpose: In (hemoglobin, Hb) HbEβ‐thalassemia, HbE (β‐26 Glu→Lys) interacts with β‐thalassemia to produce clinical manifestation of varying severity. This is the first proteomic effort to study changes in protein levels of erythrocytes isolated from HbEβ‐thalassemic patients compared to normal. Experimental design: We have used 2‐DE and MALDI‐MS/MS‐based techniques to investigate the differential proteome profiling of membrane and Hb‐depleted fraction of cytosolic proteins of erythrocytes isolated from the peripheral blood samples of HbEβ‐thalassemia patients and normal volunteers. Results: Our study showed that redox regulators such as peroxiredoxin 2, Cu‐Zn superoxide dismutase and thioredoxin and chaperones such as α‐hemoglobin stabilizing protein and HSP‐70 were upregulated in HbEβ‐thalassemia. We have also observed larger amounts of membrane associated globin chains and indications of disruption of spectrin‐based junctional complex in the membrane skeleton of HbEβ‐thalassemic erythrocytes upon detection of low molecular weight fragments of β‐spectrin and decrease in β‐actin and dematin content. Conclusion and clinical relevance: We have observed interesting changes in the proteomic levels of redox regulators and chaperons in the thalassemic hemolysates and have observed strong correlation or association of the extent of such proteomic changes with HbE levels. This could be important in understanding the role of HbE in disease progression and pathophysiology.     

Autonomic nervous system and subjective ratings of strain in air-traffic control

The paper is aimed at studying the perceived strain in professional air-traffic controllers both through self-evaluation and physiological indicators from the autonomic nervous system (ANS). The number of aircraft to be monitored was the main independent variable and could evolve at random within 1 and 10. Five ANS variables were recorded continuously in 25 participants while they handled real traffic: skin potential, skin conductance, skin blood flow, skin temperature and instantaneous heart rate. The tonic level of each physiological variable was averaged to match the times spent monitoring a constant number of aircraft. After the session, participants reacted in compliance with the NASA-TLX rating scale. Subjective ratings and physiological values were closely correlated to the number of aircraft, especially when data were standardized thus reducing inter-subjects differences in baseline levels. Results provide objective information to prevent air-traffic controllers from overloaded situations as well as to improve passengers’ safety.     

Practical issues in the application of occlusion to measure visual demands imposed on drivers by in-vehicle tasks

Occlusion is a practical technique to measure the visual demand imposed by in-vehicle tasks and to assess whether a task can be resumed having been interrupted. This study describes a number of important factors and variables that need to be controlled to ensure reliability of results. Training of participants on in-vehicle tasks is found to help consistency and five training sessions are required for complex tasks. No significant differences in training with and without occlusion goggles are reported. The required sample size is dependent on the variability of the task; for those investigated an appropriate sample size is found to be 14. For in-vehicle systems that exhibit a delay in response to the user, consistency is improved when these delays are excluded from timing measurements. In terms of calculating the occlusion parameter R, the within-participant basis is most consistent by taking the ratio of the respective median total shutter open time and total task times across trial repetitions completed by one participant on each task under evaluation and, for the purposes of identifying interface designs that exhibit poor resumability, the 85th percentile value is identified as most suitable. Findings from the study are discussed in terms of future application of the occlusion technique to assess in-vehicle information systems (IVIS).     

老树开新花 Windows XP系统优化篇

Windows7/8虽然很流行,但Windows XP并不甘心退出市场,依然有很多用户在使用。作为一个老系统,微软已不怎么更新它,所以需要我们自己来做更多的优化工作了。 让ADSL用户告别假死．自定义网卡IP地 使用ADSL上网的,每次进入桌面都会假死一会。这主要是本机网卡没有指定IP地址所致,系统自动配置网卡IP地址会花费一些时间。解决方法就是手动为网卡指定IP地址。单击“开始一连接到一显示所有连接”,打开网络连接窗口,右击其中的“本地连接”,选择“属性”打开连接属性窗口。     

一种面向维文网站的动态天气图文信息服务支持系统

维吾尔文地理信息服务的研究与应用的匮乏,使维吾尔用户很难享受基于本民族语言的地理信息服务。该文以新疆动态天气图文信息服务为例,提出了一种面向维文网站的WebGIS服务解决方案。方案首先通过集成免费Google Maps API地图服务和实时免费Yahoo Weather RSS天气信息,制作新疆县域维吾尔文天气信息服务模块;其次,将该模块作为引用对象,根据用户对动态天气图文信息服务框架大小和位置的设定,个性化地嵌入到任意维吾尔文大众网站中,使更广大的维吾尔用户享受WebGIS带来的信息服务。该研究推动了WebGIS在维吾尔语语境的新应用,为新疆各族群众提供了便捷动态天气图文信息服务。