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排序方式: 共有369条查询结果,搜索用时 15 毫秒
111.
the fission cross-sections of 235U and 239Pu for Na---Be, La---Be, Na---D and Ga---D photoneutrons have been measured absolutely. The neutron source strength was measured using a manganese bath to compare the photoneutron yield from the sources with the standard source NBS-II. Fission counts were accumulated with the source positioned symmetrically between two identical fission foils in an experiment package suspended in a low-albedo laboratory. Fission fragments passing through limited solid angle apertures were recorded on polyester track-etch films. The masses of the foil deposits were determined by microbalance weighings and confirmed by thermal neutron fission and alpha counting. After making a correction for the calculated energy distribution of the source neutrons, values of 1.471 ± 0.029, 1.274 ± 0.026, 1.162 ± 0.025 and 1.195 ± 0.026 barns were obtained for the 235U fission cross-section at the source median energies of 140, 265, 770 and 964 keV, respectively. Corresponding values of 1.469 ± 0.045, 1.515 ± 0.038, 1.670 ± 0.039 and 1.643 ± 0.038 barns were determined for 239Pu. 相似文献
112.
The highly sensitive nature of surface plasmon resonance (SPR) spectroscopy and surface plasmon field-enhanced fluorescence spectroscopy (SPFS) are governed by the strong surface plasmon resonance-generated evanescent field at the metal/dielectric interface. The greatest evanescent field amplitude at the interface and the maximum attenuation of the reflectance are observed when a nonabsorbing dielectric is employed. An absorbing dielectric decreases the evanescent field enhancement at the interface. The SPR curve of an absorbing dielectric is characterized by a greater reflectance minimum and a broader curve, as compared to those of the nonabsorbing dielectric with the same refractive index. For a weakly absorbing dielectric, such as nanometer-thick surface-confined fluorophores, the absorption is too small to induce a significant change in the SPR curve. However, the presence of a minute amount of the fluorophore can be detected by the highly sensitive SPFS. The angle with the maximum fluorescence intensity of an SPFS curve is always smaller than the resonance angle of the corresponding SPR curve. This discrepancy is due to the differences of evanescent field distributions and their decay characteristics within the metal film and the dielectric medium. The fluorescence intensity in an SPFS curve can be expressed in terms of the evanescent field amplitude. Excellent correlations between the experimentally measured fluorescence intensities and the evanescent field amplitudes are observed. 相似文献
113.
This study describes the development of a surface plasmon fluorescence spectroscopy (SPFS)-based sandwich immunoassay, for the detection of free prostate-specific antigen (f-PSA). The commercial CM5 chip (Biacore) was integrated into the SPFS-based assay making use of its three-dimensionally extended dextran architecture, which offers a large protein retention capacity and alleviates the metal-induced fluorescence quenching. The performance of the f-PSA assay was investigated in buffer and in human plasma, respectively. In the human plasma, the limit of detection of f-PSA was estimated to be approximately 80 fM for 40 min of contact time, which adequately meets clinical requirements. 相似文献
114.
Knoll A Lyakhova KS Horvat A Krausch G Sevink GJ Zvelindovsky AV Magerle R 《Nature materials》2004,3(12):886-891
The kinetics of phase transitions is essential for understanding pattern formation in structured fluids. These fluids play a key role in the morphogenesis of biological cells, and they are very common in pharmaceutical products and plastic materials. Until now, it has not been possible to follow phase transitions in structured fluids experimentally in real time and with high spatial resolution. Previous work has relied on static images and indirect experimental evidence from spatially averaging scattering experiments. Simulating the processes with computer models is a further challenge because of the multiple time and length scales involved. Our movies based on in situ scanning force microscopy show the time sequence of the elementary steps of a phase transition in a fluid film of block copolymer from the cylinder to the perforated lamella phase. The movies validate a versatile simulation model that gives physical insight into the nature of the process. Our approach provides a means of improving the study and understanding of pattern formation processes in nanostructured fluids. We expect a significant impact on nanotechnology where block copolymers serve as self-organized templates for the synthesis of inorganic nanostructured materials. 相似文献
115.
116.
M. Petzold L. Berthold D. Katzer H. Knoll D. Memhard P. Meier K. -D. Lang 《Microelectronics Reliability》2000,40(8-10)
Using indentation testing, wire bond tests and electron microscopy, the influence of increased oxide films on Al metallization surfaces on the wire bonding behavior and hardness was investigated. Oxide film thickness values larger than about 20 nm obstruct the bond contact and resulted in a poor bonding quality. The presence of such films causes also a hardness increase which can be detected by current sensitive indentation test methods. Therefore, an improved indentation testing technique can be applied during the quality control of bondpad metallizations prior to wire bonding. 相似文献
117.
Jiao Zhu Giovanni Renzone Simona Arena Francesca Romana Dani Harald Paulsen Wolfgang Knoll Christian Cambillau Andrea Scaloni Paolo Pelosi 《International journal of molecular sciences》2021,22(13)
Spider mites are one of the major agricultural pests, feeding on a large variety of plants. As a contribution to understanding chemical communication in these arthropods, we have characterized a recently discovered class of odorant-binding proteins (OBPs) in Tetranychus urticae. As in other species of Chelicerata, the four OBPs of T. urticae contain six conserved cysteines paired in a pattern (C1–C6, C2–C3, C4–C5) differing from that of insect counterparts (C1–C3, C2–C5, C4–C6). Proteomic analysis uncovered a second family of OBPs, including twelve members that are likely to be unique to T. urticae. A three-dimensional model of TurtOBP1, built on the recent X-ray structure of Varroa destructor OBP1, shows protein folding different from that of insect OBPs, although with some common features. Ligand-binding experiments indicated some affinity to coniferyl aldehyde, but specific ligands may still need to be found among very large molecules, as suggested by the size of the binding pocket. 相似文献
118.
119.
Occurrence of nisin Z production in Lactococcus lactis BFE 1500 isolated from wara, a traditional Nigerian cheese product 总被引:2,自引:0,他引:2
Olasupo NA Schillinger U Narbad A Dodd H Holzapfel WH 《International journal of food microbiology》1999,53(2-3):141-152
Screening for bacteriocin production of 500 strains of lactic acid bacteria (LAB) from various African fermented foods resulted in the detection of a bacteriocin producing Lactococcus lactis (BFE 1500) isolated from a dairy product called wara. The bacteriocin inhibited not only the closely related LAB, but also strains of Listeria monocytogenes, Listeria innocua, Clostridium butyricum, Clostridium perfringens, Bacillis cereus and Staphylococcus aureus. It was heat stable even at autoclaving temperature (121 degrees C for 15 min) and was active over a wide pH range (2-10), but highest activity was observed in the lower pH range. The bacteriocin was inactivated by alpha-chymotrypsin and proteinase K, but not by other proteases. Growth kinetic assay indicated stronger growth inhibition by the bacteriocin produced by Lc. lactis BFE 1500 on L. monocytogenes WS 2250 and B. cereus DSM 2301 than with the nisin A producing strain DSM 20729. Polymerase chain reaction indicated the presence of the nisin operon in strain BFE 1500 and sequencing of its structural gene showed that Lc. lactis BFE 1500 produced the natural nisin variant, nisin Z, as indicated by the substitution of asparagine residue instead of histidine at position 27. The genetic determinants for bacteriocin production in strain BFE 1500 are located on a conjugative transposon. The ability of the bacteriocin produced by Lc. lactis BFE 1500 to inhibit a wide range of food-borne pathogens is of special interest for food safety, especially in the African environment with perennial problems of poor food hygiene. 相似文献
120.
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