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排序方式: 共有1021条查询结果,搜索用时 15 毫秒
111.
When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50-70%. This quenching phenomenon has been acknowledged for decades, but as yet, there are no simple, practical methods to control the fluorescence of dyes conjugated to proteins, especially for dyes conjugated to immunoglobulins. Here, we report that the addition of (2-hydroxypropyl)-β-cyclodextrin (HPβCD) to dye-antibody conjugates can increase fluorescence up to 2.5-fold in cell imaging and flow analysis. This method may be an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology, histochemistry, and cell biology. 相似文献
112.
Geints YE Zemlyanov AA Kabanov AM Bykova EE Apeksimov DV Bukin OA Sokolova EB Golik SS Ilyin AA 《Applied optics》2011,50(27):5291-5298
We report on the experiments on the interaction of gigawatt femtosecond laser pulses with suspended millimeter-sized water droplets. The transparent droplets experienced laser-induced breakdown and explosive boiling up and emitted a broadband radiation. This radiation covers the spectral range from 450 to 1100?nm and consists of the spectrum of laser pulse scattered and transformed by the droplet due to self-phase modulation and plasma emission produced in water during photoionization. The droplet emission spectrum showed remarkable broadening at all viewing angles and is maximal in the direction of the laser exit from the droplet. The enlargement of the droplet results in additional spectral spreading of the emitted radiation. The depth and amount of laser pulse spectral self-transformations upon propagation through the water droplet are simulated by means of numerical calculations. 相似文献
113.
Vitalii Kim Emily Gudvangen Oleg Kondratiev Luis Redondo Shu Xiao Andrei G. Pakhomov 《International journal of molecular sciences》2021,22(13)
Intense pulsed electric fields (PEF) are a novel modality for the efficient and targeted ablation of tumors by electroporation. The major adverse side effects of PEF therapies are strong involuntary muscle contractions and pain. Nanosecond-range PEF (nsPEF) are less efficient at neurostimulation and can be employed to minimize such side effects. We quantified the impact of the electrode configuration, PEF strength (up to 20 kV/cm), repetition rate (up to 3 MHz), bi- and triphasic pulse shapes, and pulse duration (down to 10 ns) on eliciting compound action potentials (CAPs) in nerve fibers. The excitation thresholds for single unipolar but not bipolar stimuli followed the classic strength–duration dependence. The addition of the opposite polarity phase for nsPEF increased the excitation threshold, with symmetrical bipolar nsPEF being the least efficient. Stimulation by nsPEF bursts decreased the excitation threshold as a power function above a critical duty cycle of 0.1%. The threshold reduction was much weaker for symmetrical bipolar nsPEF. Supramaximal stimulation by high-rate nsPEF bursts elicited only a single CAP as long as the burst duration did not exceed the nerve refractory period. Such brief bursts of bipolar nsPEF could be the best choice to minimize neuromuscular stimulation in ablation therapies. 相似文献
114.
Ruslan Kashapov Alsu Ibragimova Rais Pavlov Dinar Gabdrakhmanov Nadezda Kashapova Evgenia Burilova Lucia Zakharova Oleg Sinyashin 《International journal of molecular sciences》2021,22(13)
Encapsulation of cargoes in nanocontainers is widely used in different fields to solve the problems of their solubility, homogeneity, stability, protection from unwanted chemical and biological destructive effects, and functional activity improvement. This approach is of special importance in biomedicine, since this makes it possible to reduce the limitations of drug delivery related to the toxicity and side effects of therapeutics, their low bioavailability and biocompatibility. This review highlights current progress in the use of lipid systems to deliver active substances to the human body. Various lipid compositions modified with amphiphilic open-chain and macrocyclic compounds, peptide molecules and alternative target ligands are discussed. Liposome modification also evolves by creating new hybrid structures consisting of organic and inorganic parts. Such nanohybrid platforms include cerasomes, which are considered as alternative nanocarriers allowing to reduce inherent limitations of lipid nanoparticles. Compositions based on mesoporous silica are beginning to acquire no less relevance due to their unique features, such as advanced porous properties, well-proven drug delivery efficiency and their versatility for creating highly efficient nanomaterials. The types of silica nanoparticles, their efficacy in biomedical applications and hybrid inorganic-polymer platforms are the subject of discussion in this review, with current challenges emphasized. 相似文献
115.
Conley DJ Humborg C Rahm L Savchuk OP Wulff F 《Environmental science & technology》2002,36(24):5315-5320
Deep-water oxygen concentrations in the Baltic Sea are influenced by eutrophication, but also by saltwater inflows from the North Sea. In the last two decades, only two major inflows have been recorded and the lack of major inflows is believed to have resulted in a long-term stagnation of the deepest bottom water. Analyzing data from 1970 to 2000 at the basin scale, we show that the estimated volume of water with oxygen, <2 mL L(-1), was actually at a minimum at the end of the longest so-called stagnation period on record. We also show that annual changes in dissolved inorganic phosphate water pools were positively correlated to the area of bottom covered by hypoxic water, but not to changes in total phosphorus load, thus addressing the legacy of eutrophication on a basinwide scale. The variations in phosphorus pools that have occurred during the past decades do not reflect any human action to reduce inputs. The long residence time and internally controlled variation of the large P pool in the Baltic Sea has important implications for management of both N and P inputs into this eutrophicated enclosed basin. 相似文献
116.
117.
José Alfredo Tirado-Méndez Flavio Iturbide-Sánchez Oleg Golovin Hildeberto Jardón-Aguilar 《国际射频与微波计算机辅助工程杂志》2004,14(2):144-152
In this article, four linearizing techniques are compared with each other when they are used in a common-emitter SiGe HBT LNA. Finally, when all of them are employed together, improved linearity of the LNA is observed and more than 10 dBm of IIP3 and −5 dBm of 1-dB gain input compression point are realized when the circuit is fed with 2.4 V. © 2004 Wiley Periodicals, Inc. Int J RF and Microwave CAE 14, 144–152, 2004. 相似文献
118.
Oleg I. Belous Anatoliy I. Fisun Anatoliy A. Kirilenko Viktor K. Korneenkov Vladimir S. Miroshnichenko 《Journal of Infrared, Millimeter and Terahertz Waves》1997,18(2):445-461
Theoretical and experimental results of an investigation into a new resonant system have been obtained. This system is named the sphere-corner-echelette open resonator (SCEOR) due to the employment of a mirror that was formed by two echelettes at the angles of 45° to the resonator axis. It turns ont that this resonator is excited on the specific modes not unique to others oscillating systems. There are presented the results of the experimental research of the orotron oscillator with the SCEOR. The spectrum of this device contains only the fundamental modes such as theT E M 006,T E M 007,T E M 008. The efficiency of the orotron is improved, when all other factors are the same the orotron with a much used sphere-cylindrical open resonator. 相似文献
119.
Oleg Alexandrovich Bannykh Victor Michailovich Blinov Vladimir Borisovich Proscurin 《国际钢铁研究》1994,65(5):178-183
Phase transformations, when cooling and heating non-austenitic high-nitrogen low-carbon steels containing chromium and other alloying elements, as well as structure and mechanical properties of these steels were analyzed. It was confirmed that these steels have high temperature chromium diffusion controlled pearlitic type transformation and martensitic type transformation. Experimental high nitrogen steels after quenching and tempering provide mechanical properties of about the same level as high strength commercial alloyed steels. Features of nitrogen as an alloying element in steels discussed allow the supposition of a possible reduction of the consumption of nickel, manganese, molybdenum or tungsten in high strength alloyed steels. 相似文献
120.
An agar dilution assay was used to assess the effect of inoculum size and culture period on the susceptibility of 15 clinical isolates of Helicobacter pylori to ampicillin, erythromycin, tetracycline, chloramphenicol, metronidazole and tinidazole. The mean MIC of the isolates increased 2.2- to 21.2-fold as the inoculum size progressed from 10(3) to 10(7) cfu/spot. Identical results were noted when isolates were maintained for two or four days prior to testing. Inoculum size should be carefully controlled when assessing the in vitro susceptibility of Helicobacter pylori. 相似文献