Optically amplified first window systems experiments

A diode pumped, commercially packaged, thulium doped fluoride fiber amplifier (TDFFA) operating in the region of 0.8 μm has been characterized. The unit is capable of producing large small signal gains, low noise figures and high saturated output powers. A power amplifier slope efficiency of 62% has been achieved at 810 nm. The same TDFFA unit was used in a series of systems experiments, at data rates of 310 Mb/s and 1.2 Gb/s, in the first telecommunications window. The results of this work indicate that data can be transmitted over ≈10 km lengths of both singlemode and standard transmission fiber, while still allowing significant margin to split the signal to a number of customers. The application of the TDFFA could therefore have significant benefit for future LAN/MAN type systems architectures involving large signal splits to customer's premises     

Identification of elements on GeneX-secA RNA of Escherichia coli required for SecA binding and secA auto-regulation

The protein translocation ATPase of Escherichia coli, SecA protein, auto-regulates its translation by binding to its translation initiation region in geneX-secA mRNA. To analyze this regulation further the secondary structure of this portion of geneX-secA RNA was investigated utilizing structure-specific nucleases and chemical probing approaches. The results of this analysis were consistent with the existence of two adjacent helices, helix I and the lower portion of helix II, whose function in secA activation and repression, respectively, has been demonstrated. Binding of SecA protein to geneX-secA RNA or various mutant derivatives of this RNA was studied by measurement of affinity constants, RNA footprint analysis, and quantitation of auto-repression in vivo. This analysis showed that the SecA-binding site in geneX-secA RNA was remarkably large spanning a region of 96 nucleotides including a 3' portion of helix II, the secA translation initiation region and distal sequences. From the size of the SecA-binding site and the plasticity of its response to mutational alteration, it is suggested that SecA protein contains two distinct RNA-binding sites. Finally, it was shown that SecA binding was not sufficient to promote auto-regulation and that sequences both upstream (helix I) and within the binding site can contribute to auto-regulation without affecting SecA-binding affinity.     

Fecal bile acid excretion and composition in response to changes in dietary wheat bran,fat and calcium in the rat

The effect and possible interactive influence of different dietary amounts of wheat bran, fat and calcium on the fecal excretion, concentration and composition of bile acids was studied in Fischer-344 rats. The fecal bile acids were analyzed using gas-liquid chromatography. Dietary wheat bran increased both total bile acid excretion and fecal weight without changes in fecal bile acid concentration. The proportion of fecal hyodeoxycholic acid decreased with increasing dietary fiber, whereas that of lithocholic and deoxycholic acids increased significantly with fiber intake. The percent content of fecal chenodeoxycholic acid did not change. Increasing dietary fat led to an increase in bile acid excretion without changes in either fecal weight or bile acid concentration. In contrast, the level of dietary calcium did not affect the total excretion of bile acids. However, since calcium increased the fecal weight, it consequently diluted bile acids and decreased their fecal concentration. Dietary fat and calcium had no influence on fecal bile acid composition. There were no interactive effects of wheat bran, fat and calcium on fecal bile acids. The finding in this study that dietary fiber, fat and calcium induce significant changes in fecal bile acids may be of relevance to the potential of bile acids to promote carcinogenesis.     

F?deratives Identit?tsmanagement am Beispiel der elektronischen Fallakte

Für Krankenh?user sind Anwendungen für kooperativ angelegte Behandlungsprozesse wesentliche Werkzeuge um einen zeitnahen einrichtungs- und sektorübergreifenden Austausch patientenbezogener medizinischer Daten zu gew?hrleisten.     

Comparison of the resistance of C57BL/6 and C3H/He mice to infection with Mycobacterium paratuberculosis

Susceptibility of C57BL/6 (Bcgs) and C3H/HeN (Bcgr) mice to an intraperitoneal infection with Mycobacterium paratuberculosis strain 19698 was compared (by histopathology and the number of mycobacteria isolated from the spleen). Mycobacterial counts from the spleen of Bcgr mice progressively decreased over the course of infection but remained unchanged in Bcgs mice. Granulomatous lesions and acid-fast bacteria were consistently present in the liver and lymph nodes of Bcgs mice, whereas lesions were transient or absent in Bcgr mice. These results indicate that Bcgr mice are inherently resistant to M. paratuberculosis, whereas Bcgs mice are inherently susceptible. These differences may prove useful in elucidating the mechanisms of resistance and susceptibility to paratuberculosis and other mycobacterial infections.     

Performance Evaluation of Range Queries in Key Value Stores

Recently there has been a considerable increase in the number of different Key-Value stores, for supporting data storage and applications on the cloud environment. While all these solutions try to offer highly available and scalable services on the cloud, they are significantly different with each other in terms of the architecture and types of the applications, they try to support. Considering three widely-used such systems: Cassandra, HBase and Voldemort; in this paper we compare them in terms of their support for different types of query workloads. We are mainly focused on the range queries. Unlike HBase and Cassandra that have built-in support for range queries, Voldemort does not support this type of queries via its available API. For this matter, practical techniques are presented on top of Voldemort to support range queries. Our performance evaluation is based on mixed query workloads, in the sense that they contain a combination of short and long range queries, beside other types of typical queries on key-value stores such as lookup and update. We show that there are trade-offs in the performance of the selected system and scheme, and the types of the query workloads that can be processed efficiently.     

Two impossibility theorems on behaviour specification of abstract data types

Two kinds of finite specification of the behaviour of a counter data type are proved impossible.We consider the class of data types (many-sorted algebras) behaving like an encapsulated counter that can be observed only by a test for zero. It is shown that no nonempty subclass of this class can be finitely specified in observational first-order logic, which is a variant of first-order logic in which equality may not be used on encapsulated types. Secondly, it is shown that the class cannot be described exactly by a finite specification in first-order logic.An extended abstract of a part of this paper appeared as: Schoett, O.: An observational subset of first-order logic cannot speacify the behaviour of a counter, in: Choffrut, C., Jantzen, M. (eds) STACS 91. 8th Annual Symposium on Theoretical Aspects of Computer Science (Lect. Notes comput. Sci., vol. 480, pp. 499–510) Berlin Heidelberg New York: Springer 1991     

Polycyclic Aromatic Hydrocarbons Activate the Aryl Hydrocarbon Receptor and the Constitutive Androstane Receptor to Regulate Xenobiotic Metabolism in Human Liver Cells

Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants produced by incomplete combustion of organic matter. They induce their own metabolism by upregulating xenobiotic-metabolizing enzymes such as cytochrome P450 monooxygenase 1A1 (CYP1A1) by activating the aryl hydrocarbon receptor (AHR). However, previous studies showed that individual PAHs may also interact with the constitutive androstane receptor (CAR). Here, we studied ten PAHs, different in carcinogenicity classification, for their potential to activate AHR- and CAR-dependent luciferase reporter genes in human liver cells. The majority of investigated PAHs activated AHR, while non-carcinogenic PAHs tended to activate CAR. We further characterized gene expression, protein abundancies and activities of the AHR targets CYP1A1 and 1A2, and the CAR target CYP2B6 in human HepaRG hepatoma cells. Enzyme induction patterns strongly resembled the profiles obtained at the receptor level, with AHR-activating PAHs inducing CYP1A1/1A2 and CAR-activating PAHs inducing CYP2B6. In summary, this study provides evidence that beside well-known activation of AHR, some PAHs also activate CAR, followed by subsequent expression of respective target genes. Furthermore, we found that an increased PAH ring number is associated with AHR activation as well as the induction of DNA double-strand breaks, whereas smaller PAHs activated CAR but showed no DNA-damaging potential.