Influence of a joining helix on the BLUF domain of the YcgF photoreceptor from Escherichia coli

BLUF-domain-comprising photoreceptors sense blue light by utilizing FAD as a chromophore. The ycgF gene product of Escherichia coli is composed of a N-terminal BLUF domain and a C-terminal EAL domain, with the latter postulated to catalyze c-di-GMP hydrolysis. The linkage between these two domains involves a predominantly helical segment. Its role on the function of the YcgF photoreceptor domain was examined by characterizing BLUF domains with and without this segment and reconstituting them with either FAD, FMN or riboflavin. The stability of the light-adapted state of the YcgF BLUF domain depends on the presence of this joining, helical segment and the adenosine diphosphate moiety of FAD. In contrast to other BLUF domains, two-dimensional (1)H,(15)N and one-dimensional (1)H NMR spectra of isotope-labeled YcgF-(1-137) revealed large conformational changes during reversion from the light- to the dark-adapted state. Based on these results the function of the joining helix in YcgF during signal transfer and the role of the BLUF domain in regulating c-di-GMP levels is discussed.     

Loading of a unilateral temporomandibular joint prosthesis: a three-dimensional mathematical study

The load on the prosthetic side and the influence of the design on the remaining natural contralateral TMJ must be known before a unilateral temporomandibular joint (TMJ) prosthesis can be developed. The aim of the present study was to determine the maximum loading of the TMJ prosthetic side and the natural contralateral TMJ and to investigate the influence of the location of the center of rotation of the prosthesis on the maximum loading. For this purpose, a mathematical model of the mandible with a unilateral TMJ prosthesis with a fixed center of rotation (CR) was developed. The location of the CR of the TMJ prosthesis was varied from the middle of the natural mandibular condyle to 15 mm inferior to this location. Although the maximum joint reaction forces changed as a result of a unilateral TMJ prosthesis, the trend of the loading curves was similar to that of an intact mandible. A unilateral TMJ prosthesis resulted in a 50% higher loading of the prosthetic side, while the load on the natural contralateral TMJ remained within normal limits. The maximum load on the prosthetic side occurred during molar bites and could reach 100 N in the cranial direction, 30 N in the ventral direction, and 25 N in the medio-lateral direction. The location of the CR did not have a significant influence on the loading of the TMJ prosthesis and the natural contralateral TMJ.     

Exposure of fertile chicken eggs to microwave radiation (2.45 GHz, CW) during incubation: technique and evaluation.

A multi-mode cavity constructed of 22-gauge perforated galvanized steel with a horn irradiator was used to expose chicken embryos to microwave (MW) radiation during incubation. The MW exposure system was placed within an environmental chamber. Mean +/- standard error of mean (SEM) power level was 3.6 +/- 0.02 mW/cm2 and mean egg specific absorption rate was 0.8 mW/g per mW/cm2. Mean temperature of the MW-exposed eggs was 37.5 +/- 0.9 degrees C as monitored by a Luxtron fluoroptic thermometer. Non-irradiated eggs were incubated at 37.5 +/- 0.1 degrees C and 55% relative humidity. There was no significant different in percent fertile eggs hatched between eggs exposed to MW radiation during incubation or eggs incubated conventionally (82.9% and 87.7%, respectively).     

Measurement and data-processing approach for estimating the spatial statistics of turbulence-induced index of refraction fluctuations in the upper atmosphere

We present a method of data reduction and analysis that has been developed for a novel experiment to measure the spatial statistics of atmospheric turbulence in the tropopause. We took measurements of temperature at 15 points on a hexagonal grid for altitudes from 12,000 to 18,000 m while suspended from a balloon performing a controlled descent. From the temperature data we estimate the index of refraction and study the spatial statistics of the turbulence-induced index of refraction fluctuations. We present and evaluate the performance of a processing approach to estimate the parameters of isotropic models for the spatial power spectrum of the turbulence. In addition to examining the parameters of the von Kármán spectrum, we have allowed the so-called power law to be a parameter in the estimation algorithm. A maximum-likelihood-based approach is used to estimate the turbulence parameters from the measurements. Simulation results presented here show that, in the presence of the anticipated levels of measurement noise, this approach allows turbulence parameters to be estimated with good accuracy, with the exception of the inner scale.     

An Accurate Distance-Calculation Algorithm for Convex Polyhedra

The paper describes an efficient and accurate algorithm to calculate the distance between convex polyhedra. The closest points between two objects can be calculated by simple projections and can be followed continuously in time. The polyhedra are given by the vertices. Interval data are supported. The accuracy of the calculation is explored.     

Wavelength controlled InAs/InP quantum dots for telecom laser applications

This article reviews the recent progress in the growth and device applications of InAs/InP quantum dots (QDs) for telecom applications. Wavelength tuning of the metalorganic vapor-phase epitaxy grown single layer and stacked InAs QDs embedded in InGaAsP/InP (1 0 0) over the 1.55-μm region at room temperature (RT) is achieved using ultra-thin GaAs interlayers underneath the QDs. The GaAs interlayers, together with reduced growth temperature and V/III ratio, and extended growth interruption suppress As/P exchange to reduce the QD height in a controlled way. Device quality of the QDs is demonstrated by temperature-dependent photoluminescence (PL) measurements, revealing zero-dimensional carrier confinement and defect-free InAs QDs, and is highlighted by continuous-wave ground-state lasing at RT of narrow ridge-waveguide QD lasers, exhibiting a broad gain spectrum. Unpolarized PL from the cleaved side, important for realization of polarization insensitive semiconductor optical amplifiers, is obtained from closely stacked QDs due to vertical electronic coupling.