Consequences of the photodynamic treatment of resting and activated peripheral T lymphocytes

Silencing of the cryptic mating-type loci HMR and HML requires the recognition of DNA sequence elements called silencers by the Sir1p, one of four proteins dedicated to the assembly of silenced chromatin in Saccharomyces cerevisiae. The Sir1p is thought to recognize silencers indirectly through interactions with proteins that bind the silencer DNA directly, such as the origin recognition complex (ORC). Eight recessive alleles of SIR1 were discovered that encode mutant Sir1 proteins specifically defective in their ability to recognize the HMR-E silencer. The eight missense mutations all map within a 17-amino-acid segment of Sir1p, and this segment was also required for Sir1p's interaction with Orc1p. The mutant Sir1 proteins could function in silencing if tethered to a silencer directly through a heterologous DNA-binding domain. Thus the amino acids identified are required for Sir1 protein's recognition of the HMR-E silencer and interaction with Orc1p, but not for its ability to function in silencing per se. The approach used to find these mutations may be applicable to defining interaction surfaces on proteins involved in other processes that require the assembly of macromolecular complexes.     

Evidence for compliance controlled oxygen vacancy and metal filament based resistive switching mechanisms in RRAM

We present electrical evidence on asymmetric metal-insulator-semiconductor (MIS) based test structures in support of the presence of two different independent switching mechanisms in a resistive random access memory (RRAM) device. The valid mechanism for switching depends on the compliance capping (I_gl) for forming/SET transition. Our results convincingly show that low compliance based switching only involves reversible oxygen ion drift to and from oxygen gettering gate electrodes, while high compliance switching involves formation and rupture of conductive metallic nanofilaments, as verified further by our physical analysis investigations. We have observed this unique dual mode switching mechanism only in NiSi-based gate electrodes, which have a moderate oxygen solubility as well as relatively low melting point.     

Continuous spatial assignment of moving users

Consider a set of servers and a set of users, where each server has a coverage region (i.e., an area of service) and a capacity (i.e., a maximum number of users it can serve). Our task is to assign every user to one server subject to the coverage and capacity constraints. To offer the highest quality of service, we wish to minimize the average distance between users and their assigned server. This is an instance of a well-studied problem in operations research, termed optimal assignment. Even though there exist several solutions for the static case (where user locations are fixed), there is currently no method for dynamic settings. In this paper, we consider the continuous assignment problem (CAP), where an optimal assignment must be constantly maintained between mobile users and a set of servers. The fact that the users are mobile necessitates real-time reassignment so that the quality of service remains high (i.e., their distance from their assigned servers is minimized). The large scale and the time-critical nature of targeted applications require fast CAP solutions. We propose an algorithm that utilizes the geometric characteristics of the problem and significantly accelerates the initial assignment computation and its subsequent maintenance. Our method applies to different cost functions (e.g., average squared distance) and to any Minkowski distance metric (e.g., Euclidean, L ₁ norm, etc.).     

Effect of nano-TiO<Subscript>2</Subscript> addition on the microstructure and bonding strengths of Sn3.5Ag0.5Cu composite solder BGA packages with immersion Sn surface finish

The effects of nano-TiO₂ particles on the interfacial microstructures and bonding strength of Sn3.5Ag0.5Cu composite solder joints in ball grid array packages with immersion Sn surface finishes have been investigated. Metallography reveals that addition of nano-TiO₂ particle retarded wicker-Cu₆Sn₅ IMC formed in the Sn3.5Ag0.5Cu composite solder joints. The thickness of the interfacial intermetallic compounds of the solder joint was reduced with increased additions of nano-TiO₂ particles (0.25–1.0 wt%), but further additions up to 1.25 wt% decreased the beneficial influence. This indicates that the presence of a small amount of nano-TiO₂ particles is effective in suppressing the growth of the intermetallic compounds layer. In addition, the shear strength of the soldered joints was improved by larger nano-TiO₂ particle additions, with the peak shear strength occurring at 1.0 wt% of nano-TiO₂ particles into the Sn3.5Ag0.5Cu solder. The fracture mode also changed with increased amounts of nano-TiO₂ particles.     

Digital signal processing in communications satellite payloads

Satellite on-board digital signal processing (DSP) offers the potential for significantly improved performance in certain satellite communications system scenarios in terms of system capacity and link quality. On-board DSP functions include digital beamforming, frequency multiplexing and demultiplexing, interference suppression, signal level control and, in a regenerative system, modems. The authors review the status of DSP in communications satellite payloads, focusing on the system advantages, some basic algorithms, and application specific IC implementation issues. As a specific illustrative example an advanced land mobile system is described which features digital beamforming and associated digital signal processing to achieve significant gains in power efficiency (and hence capacity), frequency reuse and flexibility to response to changing traffic scenarios     

A Haptic Knob for Rehabilitation of Hand Function

This paper describes a novel two-degree-of-freedom robotic interface to train opening/closing of the hand and knob manipulation. The mechanical design, based on two parallelogram structures holding an exchangeable button, offers the possibility to adapt the interface to various hand sizes and finger orientations, as well as to right-handed or left-handed subjects. The interaction with the subject is measured by means of position encoders and four force sensors located close to the output measuring grasping and insertion forces. Various knobs can be mounted on the interface, including a cone mechanism to train a complete opening movement from a strongly contracted and closed hand to a large opened position. We describe the design based on measured biomechanics, the redundant safety mechanisms as well as the actuation and control architecture. Preliminary experiments show the performance of this interface and some of the possibilities it offers for the rehabilitation of hand function.     

Converting baluns into broad-band impedance-transforming 180/spl deg/ hybrids

A technique for converting baluns into 180/spl deg/ hybrids by adding an in-phase power splitter is presented in this paper. Incorporating the broad-band antiphase and in-phase power splitting characteristics of the balun and power splitter results in a 180/spl deg/ hybrid with broad-band characteristics. This technique also provides a means of achieving perfect matching and output isolation for three-port lossless baluns. Applying this technique to a Marchand balun will result in a broad-band impedance-transforming 180/spl deg/ hybrid. Simple design equations based on the scattering matrix are presented. These theoretical results are validated by an experimental 180/spl deg/ hybrid using a coupled line Marchand balun. It achieves amplitude balance of 0.5 dB and phase balance of less than 5/spl deg/ from 1.2 to 3.2 GHz.     

Digital wireless sensor server using an adaptive smart-antenna/retrodirective array

A wireless sensor server is developed based on a reconfigurable active smart-antenna/retrodirective array. The system can serve as both a retrodirective array transponder and a smart-antenna receiver simply by changing the frequency of the local oscillator applied to the mixers, enabling it to utilize its hardware best to suit its communication environment. When operating as a direct-conversion receiver, the receiver array successfully demodulates a quaternary phase-shift keying (QPSK) modulated signal with circuit gain of 7 dB, and E/sub b//N/sub 0/ for BER=10/sup -4/ is approximately 12 dB without any error correction. In the retrodirective array mode, the system provides 20 dB circuit gain and 20 dB radio-frequency/intermediate-frequency isolation at the center frequency as well as phase conjugation, exhibiting excellent retrodirectivity. The mixers perform phase conjugation and modulation simultaneously, enabling the transmission of locally stored data. The local data is successfully extracted by an interrogator.     

Microwave properties of low-loss polymers at cryogenic temperatures

Dielectric loss tangent and permittivity of polytetrafluoroethylene (Teflon), high-density (HD) polyethylene, and cross-linked polystyrene (Rexolite) were measured at temperature range from 28 to 84 K and frequency of approximately 18 GHz. The material properties were determined by measurements of the resonant frequency and the Q factor of a TE₀₁₁ mode cylindrical superconducting cavity containing a sample under test. It has been demonstrated that these materials exhibit very low losses at cryogenic temperatures (2×10^-6 for Teflon, 5×10^-5 for HD polyethylene and 1.1×10^-4 for Rexolite). Due to low losses, these materials can be useful in construction of various high-Q factor microwave devices for operation at cryogenic temperatures     

Randomized controlled trial of open and closed haemorrhoidectomy

BACKGROUND: This study was a prospective randomized comparison of healing following open and closed haemorrhoidectomy. METHODS: Sixty-seven consecutive patients (mean(s.e.m.) age 45(1.7) years; 35 men, 32 women) with three prolapsed piles were randomized to open haemorrhoidectomy (n = 34) or closed haemorrhoidectomy (n = 33). RESULTS: Mean(s.e.m.) follow-up was 8.7(0.2) months. There were no differences in the linear analogue pain scores, analgesic requirements and length of hospitalization after open haemorrhoidectomy and closed haemorrhoidectomy. Complete wound healing took significantly longer after closed haemorrhoidectomy (mean(s.e.m.) 6.9(0.7) weeks) compared with open haemorrhoidectomy (4.9(0.4)weeks) (P < 0.05). This was related to wound dehiscence in eight patients. Complication rates, however, were similar except for prolonged serous discharge from unhealed wounds. The anal manometry findings after both procedures were equivalent. CONCLUSION: Open haemorrhoidectomy leads to faster and more reliable wound healing, although this did not result in less pain or fewer complications.