Characterization of expression of phosphofructokinase isoforms in isolated rat pancreatic islets and purified beta cells and cloning and expression of the rat phosphofructokinase-A isoform

We prepared polymers having a phospholipid polar group, poly [omega-methacryloyloxyalkyl phosphorylcholine (MAPC)-co-n-butyl methacrylate(BMA)], as new biomedical materials and evaluated their blood compatibility with attention to protein adsorption and platelet adhesion. The total amount of proteins adsorbed on the polymer surface from human plasma was determined, and the distribution of adsorbed proteins on the plasma-contacting surface was analyzed. The amount of proteins adsorbed on every poly (MAPC-co-BMA) was small compared with that observed on polymers without the phospholipid polar group. However, there was no significant difference in the amount of adsorbed proteins on the poly(MAPC-co-BMA) even when the methylene chain length between the phospholipid polar group and the backbone in the MAPC moiety was altered. Platelet adhesion on the polymer surface from a platelet suspension in a buffered solution was evaluated with and without plasma treatment on the surface. When a rabbit platelet suspension was brought into contact with the poly(BMA) surface after treatment with plasma, many platelets adhered and aggregated. However, a reduced amount of platelet adhered on the poly(BMA) was found in the case of direct contact with the platelet suspension. On the other hand, the poly(MAPC-co-BMA)s could inhibit platelet adhesion under both conditions. Based on these results, it can be concluded that the proteins adsorbed on the surface play an important role in determining the platelet adhesion and suppression of the protein adsorption on the surface, which is one of the most significant ways of inhibiting platelet adhesion.     

c-KIT expression enhances the leukemogenic potential of 32D cells

The growth of human leukemic cells in culture and in vivo is dependent upon the presence of hematopoietic growth factors. Most populations of human leukemic acute myeloblastic leukemia (AML) cells express c-Kit on their surface and respond to Kit ligand (KL) in culture. To determine if this interaction was of potential significance in vivo we used a mouse model system. 32D cells, a murine IL-3-dependent myeloid cell line, were rendered KL responsive by transfection of the murine c-Kit. After injection of 32D or 32D-Kit cells into syngeneic hosts, animals bearing 32D-Kit cells, but not 32D cells, became moribund and were killed. These animals had circulating leukemic blast cells, infiltration of bone marrow, spleen, brain, liver, lung, and kidney. Cells recovered from some of the animals continued to be dependent upon IL-3 or KL for growth while in other cases the cells were factor independent. This model illustrates that the constitutive expression of c-Kit enhances the leukemic potential of 32D cells. The model will be useful for studying the progression of leukemia in vivo and testing whether interruption of the interaction of Kit and KL can affect the growth of leukemic cells.     

Air bearing design, optimization, stability analysis andverification for sub-25 nm flying

Three sub-25 nm fly height sliders are presented for near contact recording. The designs are geared towards the goal of achieving 10 Gb/in ² areal density. The optimization procedure presented shows promise for facilitating achievement of this goal. The dynamic simulations show the stability of these designs when disturbed from their steady state conditions     

中国民航信息系统现状及发展展望

介绍了中国民航信息系统的现状和与实际业务需求的差距,接着从系统设计理念、核心系统、新系统新功能和信息系统整合等多个方面详细阐述了民航信息系统的发展思路,最后强调了建立新一代民航信息系统的紧迫性,具备很强的指导意义。     

ICP-AES法测定金属镝中钽

利用钽不溶于酸的性质与稀土分离，用焦硫酸钾熔融，酒石酸浸取，制得被测溶液，采用ICP-AES测定其中钽量，结果令人满意。     

浅谈“水源空调“在丹东市的推广使用

本文通过对“水源空调”的介绍,结合丹东市的地质情况,分析论证其在我市推广使用的可行性。     

国际工程中的安全管理——浅谈安全管理在巴贡电站工程施工中的应用

旨在通过对马来西亚巴贡水电站工程安全管理的介绍与分析,结合国内施工企业在安全管理方面的特点,找出了适合我国施工企业可以借鉴的经验与管理要点,旨在提高国内施工企业的安全管理水平.     

潜孔锤冲击作用下土体变形的有限元分析

潜孔锤冲击挤密钻进过程中周围土体的变形机理是一个高度非线性问题。针对潜孔锤在土中的面-面接触问题,运用Drucker-Prager屈服准则为判据,将土体视为DP材料,通过钻具与土之间的接触及施加冲击载荷来模拟钻进过程,从而建立了潜孔锤在土中冲击挤密钻进的有限元模型。利用非线性瞬态动力学有限元分析手段,对钻进过程中冲击力对土的作用加以研究,分析了钻具周围土体的应力、应变以及钻具与土体的摩擦情况。结果表明,在冲击载荷作用下,锥形钻头前端土体应力集中,并形成应力泡,冲击功主要用于竖向压缩土体,而水平方向的挤压作用相对较小,摩擦应力在钻头锥面附近出现峰值,是影响钻进速度的主要原因之一。