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51.
L Zardi LS Vincent A Barbin R Montesano GP Margison 《Canadian Metallurgical Quarterly》1977,3(3-4):183-188
DNA repair was measured in human fibroblasts, mouse C3H 10 T 1/2 fibroblsts and rat hepatocytes by the non-semi-conservative incorporation of [3H]-TdR during DNA repair synthesis using liquid scintillation techniques. Confluent monolayers of these cells grown on cover slips were exposed to split doses (125 or 250 microgram/ml) of the mutagenic and carcinogenic alkylating agent MNU and DNA repair synthesis compared with that produced by a single dose (500 microgram/ml). No significant difference in DNA repair capacity was detected in the three cell lines treated with a single dose or split doses of MNU. 相似文献
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After drinking a small dose of alcohol, Orientals flushed, their heart rates increased and their diastolic pressures decreased; Caucasians' skin reflectance did not change, but their heart rates and systolic blood pressures decreased. In both groups, flushing was associated with an increase in feelings of vigor. Other mood correlates depended on condition. 相似文献
53.
Regimes of parallel and turbulent flow in a parallel plate precipitator are discussed as theoretical idealizations. Particle trajectories and resultant efficiency of an ideal parallel flow device are described and compared with the Deutsch equation analysis, based upon complete mixing in turbulent flow, for a single mobility species. A relationship is developed between particle size and mobility which incorporates field and diffusion charging and the Cunningham correction to Stoke's drag. Log normal distributions of particle size are transformed into mobility distribution functions. Efficiencies based upon parallel flow and turbulent mixing are expressed for the particle mobility distributions by finding incremental efficiencies for each segment of a given distribution and weighting each segment by its relative proportion of the whole. These efficiencies are compared with each other and with the result obtained by inserting the mean mobility into the Deutsch equation. It is shown that, for relatively high standard deviation, use of an average mobility value in the Deutsch equation can result in device efficiency characteristics quite similar to those resulting from collection of the various mobility species in parallel flow. A much lower efficiency results from incremental analysis of the same distribution under the assumption of turbulent mixing. 相似文献
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GP Walsh 《Canadian Metallurgical Quarterly》1996,347(9016):1690-1691
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TE Cawston VA Curry CA Summers IM Clark GP Riley PF Life JR Spaull MB Goldring PJ Koshy AD Rowan WD Shingleton 《Canadian Metallurgical Quarterly》1998,41(10):1760-1771
OBJECTIVE: To study the interaction of interleukin-1alpha (IL-1alpha) and oncostatin M (OSM) in promoting cartilage collagen destruction. METHODS: Bovine, porcine, and human cartilage and human chondrocytes were studied in culture. The levels of collagenase (matrix metalloproteinase 1 [MMP-1]) and tissue inhibitor of metalloproteinases 1 (TIMP-1) were measured by bioassay and enzyme-linked immunosorbent assay (ELISA). The levels of OSM in rheumatoid synovial fluid were measured by ELISA. RESULTS: When combined with OSM, IL-1alpha, IL-1beta, and tumor necrosis factor alpha released proteoglycan and collagen from cartilage. OSM was the only member of the IL-6 family to have this effect. Human tendon also responded to IL-1alpha and OSM. OSM increased the production of MMP-1 and TIMP-1 but when combined with IL-1alpha, synergistically promoted MMP-1 production in human chondrocytes and synovial fibroblasts. High levels of OSM were found in human rheumatoid synovial fluids, and confocal microscopy showed that OSM was produced by macrophages in rheumatoid synovial tissue. CONCLUSION: These results highlight an important new mechanism by which there is irreversible loss of collagen from cartilage. 相似文献
59.
PR Gibson D Kilias O Rosella JM Day M Abbott CF Finch GP Young 《Canadian Metallurgical Quarterly》1998,94(6):671-676
Public awareness and misunderstandings of lactose intolerance are at an all-time high. Many people erroneously believe they are lactose intolerant or develop gastrointestinal symptoms after intake of lactose. Consequently, lactose-containing foods such as milk and other dairy foods may be eliminated unnecessarily from the diet. Because these foods are a major source of calcium, low intake of them can compromise calcium nutriture. This, in turn, can increase the risk of major chronic diseases such as osteoporosis (porous bones) and hypertension. This review is intended to help dietetics professionals alleviate clients' fears about lactose intolerance and recommend dietary strategies to improve tolerance to lactose. Scientific findings indicate that the prevalence of lactose intolerance is grossly overestimated. Other physiologic and psychologic factors can contribute to gastrointestinal symptoms that mimic lactose intolerance. Scientific findings also indicate that people with laboratory-confirmed low levels of the enzyme lactase can consume 1 serving of milk with a meal or 2 servings of milk per day in divided doses at breakfast and dinner without experiencing symptoms. Several dietary strategies are available to help lactose maldigesters include milk and other dairy foods in their diet without experiencing symptoms. 相似文献
60.
C Mannironi A Di Nardo P Fruscoloni GP Tocchini-Valentini 《Canadian Metallurgical Quarterly》1997,36(32):9726-9734
RNA aptamers that specifically bind dopamine have been isolated by in vitro selection from a pool of 3.4 x 10(14) different RNA molecules. One aptamer (dopa2), which dominated the selected pool, has been characterized and binds to the dopamine affinity column with a dissociation constant of 2.8 microM. The specificity of binding has been determined by studying binding properties of a number of dopamine-related molecules, showing that the interaction with the RNA might be mediated by the hydroxyl group at position 3 and the proximal aliphatic chain in the dopamine molecule. The binding domain was initially localized by boundary experiments. Further definition of the dopamine binding site was obtained by secondary selection on a pool of sequences derived from a partial randomization of the dopa2 molecule. Sequence comparison of a large panel of selected variants revealed a structural consensus motif among the active aptamers. The dopamine binding pocket is built up by a tertiary interaction between two stem and loop motifs, creating a stable framework in which five invariant nucleotides are precisely arrayed. Minimal active sequence and key nucleotides have been confirmed by the design of small functional aptamers and mutational analysis. Enzymatic probing suggests that the RNA might undergo a conformational change upon ligand binding that stabilizes the proposed tertiary structure. 相似文献