Spontaneous tibial artery thrombosis associated with varicella pneumonia and free protein S deficiency

Pneumonia is the most common serious complication of varicella infection in adults. A variety of thrombotic complications including purpura fulminans and disseminated intravascular coagulation have been reported in children with varicella but not in adults. Two men with varicella pneumonia who had profound lower extremity ischemia caused by thrombosis of the profunda femoris and tibial arteries are reported. Both patients had free protein S deficiency and vascular thrombosis in association with varicella pneumonia without overt evidence of disseminated intravascular coagulation or purpura fulminans. Antiphospholipid immunoglobulin G and immunoglobulin M antibodies were present in one, whereas the other had evidence of the lupus anticoagulant. The proposed pathogenesis and management options including intraarterial thrombolytic therapy with urokinase and the need for long-term anticoagulation are discussed.     

The effects of automobile inspections on accident rates

To determine whether diagnostic inspections reduce assidents, this paper compares the accident rates of vehicles which participated in the Alabama Motor Vehicle Diagnostic Inspection Demonstration Program with vehicles which did not actively participate. In Huntsville the accident rate of inspected vehicles represents an improvement of 9.1% over the rate for uninspected vehicles. Furthermore, the accident rate of inspected vehicles decreased 5.3% after inspection. Whereas the monthly accident rate of the responsive participant who returned for subsequent periodic inspections did not significantly increase in eighteen months, the monthly accident rate of unresponsive participants increased to the level of uninspected vehicles. Vehicles involved in accidents were in significantly worse mechanical condition on the average than those not involved in accidents. The data suggests that poor mechanical condition is a significant factor in motor vehicle accidents and that annual inspections are a desirable means of reducing accident rates.     

Measurement and modeling of the electromagnetic response to phase transformation in steels

An electromagnetic (EM) sensor, capable of detecting the formation of ferromagnetic ferrite from paramagnetic austenite below the Curie temperature, has been developed and assessed. In this article, results obtained using an a.c. EM sensor for a medium (0.45 wt pct)-carbon steel slow cooled through its transformation-temperature range are presented. It was found that the EM sensor can successfully detect the formation of ferrite below the Curie temperature, but that the transimpedance values can be significantly affected by the formation of a decarburized ferrite ring around the samples. It was also found that the transimpedance value is monotonically (nonlinearly) related to the ferrite volume fraction and depends on the morphology/distribution of the ferromagnetic phase and, hence, is influenced by the prior-austenite grain size. Results from finite-element (FE) simulations designed to enable prediction of the transimpedance from the microstructure are also presented, showing that two-dimensional (2-D) FE simulations can be successfully used to model the experimental trends observed. The combination of modeling and measurement has shown that EM sensors can be used to indirectly monitor the ferrite transformation (below the Curie temperature), thus providing a measure of ferrite volume fraction and also a means of identifying the ferrite distribution in the microstructure.     

Effects of cell condition, pH, and temperature on lead, zinc, and copper sorption to Acidithiobacillus caldus strain BC13

This study describes the effects of cell condition, pH, and temperature on lead, zinc, and copper sorption to Acidithiobacillus caldus strain BC13 with a Langmuir model. Copper exhibited the highest loading capacity, 4.76 ± 0.28 mmol g(-1), to viable cells at pH 5.5. The highest k(L) (binding-site affinity) observed was 61.2 ± 3.0 L mmol(-1) to dehydrated cells at pH 4.0. The pHs that maximized loading capacities and binding-site affinities were generally between 4.0 and 5.5, where the sum of free-proton and complexed-metal concentrations was near a minimum. Of additional importance, lead, zinc, and copper sorbed to viable cells at pH values as low as 1.5. Previous studies with other acidithiobacilli did not measure viable-cell sorption below pH 4.0. In separate experiments, desorption studies showed that far less copper was recovered from viable cells than any other metal or cell condition, suggesting that uptake may play an important role in copper sorption by At. caldus strain BC13. To reflect an applied system, the sorption of metal mixtures was also studied. In these experiments, lead, zinc, and copper sorption from a tertiary mixture were 40.2 ± 4.3%, 28.7 ± 3.8%, and 91.3 ± 3.0%, respectively, of that sorbed in single-metal systems.     

Efficient computation of higher order frequency response functions for nonlinear systems with,and without,a constant term

The behaviour of a nonlinear system can be profoundly affected by the presence of a constant or dc term in the system governing equation. These changes are reflected in the nonlinear frequency response characteristics of the system which provide a powerful insight into the system's dynamics. In this article, a new and efficient algorithm is presented for computing the higher order Volterra frequency response functions from nonlinear time-domain models that may contain a constant term. A comparison with previous methods is included to demonstrate the significant gains in computational efficiency that are achieved using the new method. The algorithm is applicable to systems modelled by nonlinear differential, or difference, equations and is easily automated. Several examples are used to illustrate the method, and to highlight the importance of dc terms in nonlinear system analysis.     

Yacc in sasl — an exercise in functional programming

Among the advantages claimed for a purely functional programming style is ease of designing and implementing large programs. However, little experience of actually doing so has been gained so far. The experience of writing a particular medium sized program in a functional language is described here, with particular emphasis on the differences in programming style that were appropriate. This is compared with the experience of writing a very similar program in an imperative language. The main conclusions appear to be that the functional version was significantly easier to write, and was considerably smaller, though not by as large a factor as is sometimes claimed; that strong typing is, if anything, more desirable than in imperative systems; and that the question of debugging functional programs needs further research attention.     

State in Haskell

Some algorithms make critical internal use of updatable state, even though their external specification is purely functional. Based on earlier work on monads, we present a way of securely encapsulating stateful computations that manipulate multiple, named, mutable objects, in the context of a non-strict, purely-functional language. The security of the encapsulation is assured by the type system, using parametricity. The same framework is also used to handle input/output operations (state changes on the external world) and calls to C.     

A Patch of Detachable Hybrid Microneedle Depot for Localized Delivery of Mesenchymal Stem Cells in Regeneration Therapy

Mesenchymal stem cells (MSCs) have been widely used for regenerative therapy. In most current clinical applications, MSCs are delivered by injection but face significant issues with cell viability and penetration into the target tissue due to a limited migration capacity. Some therapies have attempted to improve MSC stability by their encapsulation within biomaterials; however, these treatments still require an enormous number of cells to achieve therapeutic efficacy due to low efficiency. Additionally, while local injection allows for targeted delivery, injections with conventional syringes are highly invasive. Due to the challenges associated with stem cell delivery, a local and minimally invasive approach with high efficiency and improved cell viability is highly desired. In this study, a detachable hybrid microneedle depot (d‐HMND) for cell delivery is presented. The system consists of an array of microneedles with an outer poly(lactic‐co‐glycolic) acid shell and an internal gelatin methacryloyl (GelMA)‐MSC mixture (GMM). The GMM is characterized and optimized for cell viability and mechanical strength of the d‐HMND required to penetrate mouse skin tissue is also determined. MSC viability and function within the d‐HMND is characterized in vitro and the regenerative efficacy of the d‐HMND is demonstrated in vivo using a mouse skin wound model.     

Comparison of uranium(VI) removal by Shewanella oneidensis MR-1 in flow and batch reactors

To better understand the interactions among metal contaminants, nutrients, and microorganisms in subsurface fracture-flow systems, biofilms of pure culture of Shewanella oneidensis MR-1 were grown in six fracture-flow reactors (FFRs) of different geometries. The spatial and temporal distribution of uranium and bacteria were examined using a tracer dye (brilliant blue FCF) and microscopy. The results showed that plugging by bacterial cells was dependent on the geometry of the reactor and that biofilms grown in FFRs had a limited U(VI)-reduction capacity. To quantify the U(VI)-reduction capacity of biofilms, batch experiments for U(VI) reduction were performed with repetitive U(VI) additions. U(VI)-reduction rates of stationary phase cultures decreased after each U(VI) addition. After the fourth U(VI) addition, stationary phase cultures treated with U(VI) with and without spent medium yielded gray and black precipitates, respectively. These gray and black U precipitates were analyzed using high-resolution transmission electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction. Data for randomly selected areas of black precipitates showed that reduced U particles (3-6 nm) were crystalline, whereas gray precipitates were a mixture of crystalline and amorphous solids. Results obtained in this study, including a dramatic limitation of S. oneidensis MR-1 and its biofilms to reduce U(VI) and plugging of FFRs, suggest that alternative organisms should be targeted for stimulation for metal immobilization in subsurface fracture-flow systems.