Uranium immobilization by sulfate-reducing biofilms

Hexavalent uranium [U(VI)] was immobilized using biofilms of the sulfate-reducing bacterium (SRB) Desulfovibrio desulfuricans G20. The biofilms were grown in flat-plate continuous-flow reactors using lactate as the electron donor and sulfate as the electron acceptor. U(VI)was continuously fed into the reactor for 32 weeks at a concentration of 126 microM. During this time, the soluble U(VI) was removed (between 88 and 96% of feed) from solution and immobilized in the biofilms. The dynamics of U immobilization in the sulfate-reducing biofilms were quantified by estimating: (1) microbial activity in the SRB biofilm, defined as the hydrogen sulfide (H2S) production rate and estimated from the H2S concentration profiles measured using microelectrodes across the biofilms; (2) concentration of dissolved U in the solution; and (3) the mass of U precipitated in the biofilm. Results suggest that U was immobilized in the biofilms as a result of two processes: (1) enzymatically and (2) chemically, by reacting with microbially generated H2S. Visual inspection showed that the dissolved sulfide species reacted with U(VI) to produce a black precipitate. Synchrotron-based U L3-edge X-ray absorption near edge structure (XANES) spectroscopy analysis of U precipitated abiotically by sodium sulfide indicated that U(VI) had been reduced to U(IV). Selected-area electron diffraction pattern and crystallographic analysis of transmission electron microscope lattice-fringe images confirmed the structure of precipitated U as being that of uraninite.     

Reoxidation of reduced uranium with iron(III) (hydr)oxides under sulfate-reducing conditions

In cultures of Desulfovibrio desulfuricans 620 the effects of iron(III) (hydr)oxides (hematite, goethite, and ferrihydrite) on microbial reduction and reoxidation of uranium (U) were evaluated under lactate-limited sulfate-reducing conditions. With lactate present, G20 reduced U(VI) in both 1,4-piperazinediethanesulfonate (PIPES) and bicarbonate buffer. Once lactate was depleted, however, microbially reduced U served as an electron donor to reduce Fe(III) present in iron(III) (hydr)oxides. With the same initial amount of Fe(III) (10 mmol/L) for each iron(III) (hydr)oxide, reoxidation of U(IV) was greater with hematite than with goethite orferrihydrite. As the initial mass loading of hematite increased from 0 to 20 mmol of Fe(III)/L, the rate and extent of U(IV) reoxidation increased. Subsequent addition of hematite [15 mmol of Fe(III)/L] to stationary-phase cultures containing microbially reduced U(IV) also resulted in rapid reoxidation to U(VI). Analysis by U L3-edge X-ray absorption near-edge spectroscopy (XANES) of microbially reduced U particles yielded spectra similar to that of natural uraninite. Observations by high-resolution transmission electron microscopy, selected area electron diffraction, and energy-dispersive X-ray spectroscopic analysis confirmed that precipitated U associated with cells was uraninite with particle diameters of 3-5 nm. By the same techniques, iron sulfide precipitates were found to have a variable Fe and S stoichiometry and were not associated with cells.     

Treating anxiety disorders in children with group cognitive-behavioral therapy: A randomized clinical trial.

A randomized clinical trial evaluated the therapeutic efficacy of group cognitive-behavioral therapy (GCBT) versus a wait-list control (WLC) condition to treat anxiety disorders in children. Results indicated that GCBT, with concurrent parent sessions, was highly efficacious in producing and maintaining treatment gains. Children in GCBT showed substantial improvement on all the main outcome measures, and these gains were maintained at 3-, 6-, and 12-month follow-ups. Children in the WLC condition did not show improvements from the pre- to postwait assessment point. These findings are discussed in terms of the need to continue to advance the development of practical, as well as conceptual, knowledge of efficacious treatment for anxiety disorders in children. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The Geography of Supermarkets in Cape Town: Supermarket Expansion and Food Access

Although the rapid expansion in the number of supermarkets in South and Southern Africa in recent years is well-documented, the potential impact of this process is not well understood. The existing literature does not engage adequately with the spatial distribution of supermarkets within cities and is therefore unable to address the impact of these stores on household food security. The paper presents a mapping of the location of supermarkets in Cape Town with reference to income characteristics of neighbourhoods and transport routes. The distribution of supermarkets is shown to be highly unequal and the distance of low-income from high-income areas hinders access to supermarkets for the urban poor. The paper further argues that the supermarkets in low-income areas typically stock less healthy foods than those in wealthier areas and, as a result, the supermarkets do not increase access to healthy foods and may, in fact, accelerate the nutrition transition.     

Imaging system based on electromagnetic tomography (EMT)

A novel non-contacting instrumentation system for obtaining cross-sectional images of conductive and ferrous material is described. The image space is energised with a parallel magnetic field and the distortion created by objects is detected by sensing coils. The backprojection method is used for reconstruction. Initial results are presented.<>     

State in Haskell

Some algorithms make critical internal use of updatable state, even though their external specification is purely functional. Based on earlier work on monads, we present a way of securely encapsulating stateful computations that manipulate multiple, named, mutable objects, in the context of a non-strict, purely-functional language. The security of the encapsulation is assured by the type system, using parametricity. The same framework is also used to handle input/output operations (state changes on the external world) and calls to C.     

Circuit for Monitoring the Median Frequency of the Spectrum of the Surface EMG Signal

The power spectrum of the surface EMG signal is known to undergo a compression towards the lower frequencies during sustained muscle contractions. The median frequency appears to be the preferred parameter to monitor this compression. This paper describes a simple circuit which can provide an estimate of the median frequency.     

Micropatterning Alginate Substrates for In Vitro Cardiovascular Muscle on a Chip

Soft hydrogels such as alginate are ideal substrates for building muscle in vitro because they have structural and mechanical properties close to the in vivo extracellular matrix (ECM) network. However, hydrogels are generally not amenable to protein adhesion and patterning. Moreover, muscle structures and their underlying ECM are highly anisotropic, and it is imperative that in vitro models recapitulate the structural anisotropy in reconstructed tissues for in vivo relevance due to the tight coupling between sturcture and function in these systems. Two techniques to create chemical and structural heterogeneities within soft alginate substrates are presented and employed to engineer anisotropic muscle monolayers: i) microcontact printing lines of extracellular matrix proteins on flat alginate substrates to guide cellular processes with chemical cues and ii) micromolding of alginate surface into grooves and ridges to guide cellular processes with topographical cues. Neonatal rat ventricular myocytes as well as human umbilical artery vascular smooth muscle cells successfully attach to both these micropatterned substrates leading to subsequent formation of anisotropic striated and smooth muscle tissues. Muscular thin film cantilevers cut from these constructs are then employed for functional characterization of engineered muscular tissues. Thus, micropatterned alginate is an ideal substrate for in vitro models of muscle tissue because it facilitates recapitulation of the anisotropic architecture of muscle, mimics the mechanical properties of the ECM microenvironment, and is amenable to evaluation of functional contractile properties.     

Nitrate reduction with Halomonas campisalis. Kinetics of denitrification at pH 9 and 12.5% NaCl

Regeneration of ion exchange resins with NaCl produces brine containing high concentrations of nitrate that can be difficult to remove using standard biological, physical, or chemical technologies. In this study. Halomonas campisalis (ATCC #700597) (Mormile et al., 1999) was shown to completely reduce nitrate at 125 g/L NaCl and pH 9. This organism was also used in experiments to determine nitrate-reduction rates and biomass yields. Kinetic parameters were measured separately with glycerol, lactate. acetate, ethanol, and methanol. The specific nitrate-reduction rate coefficient was highest in cultures amended with acetate, while lactate and glycerol (a natural osmoticum in hypersaline environments) had lower reduction rates. No evidence of nitrate reduction was observed when ethanol or methanol was provided as an electron donor. Kinetic modeling provided values for nitrate and nitrite-reduction rate coefficients and for biomass yields. Measured rates and yields were similar to reported parameters obtained from non-halophilic nitrate-reducing cultures under low salt concentrations. Therefore, for highly saline solutions, the use of halophiles to selectively remove nitrate from these brines may represent a viable treatment option.     

Exploring the relationship between ferrite fraction and morphology and the electromagnetic properties of steel

The link between the electromagnetic properties of steel and its microstructure is a complex one, depending on both phase fractions and morphology. In this paper, both analytical and three-dimensional finite element (3D FEM) modelling techniques were applied to the prediction of permeability for steel with a given ferrite fraction for random ferrite/austenite distributions. Experimental measurements from a multi-frequency electromagnetic sensor on samples generated by hot isostatic pressing (HIPping) of powder mixtures were used to evaluate the analytical and FEM predictions. Theoretical treatment of the relationship between the sensor output and the effective permeability is also given; in particular, it was found that the zero crossing frequency of the real part of the inductance is approximately linearly related to the permeability for high (> 40%) ferrite percentages. The EM sensor can therefore be used to identify the samples across the full range (0–100%) of ferrite percentages using both the zero crossing frequency (> 40%) and trans-impedance (0–40%). The effect of banded (non-random) microstructures on sensor output and the prediction of the upper and lower bounds of permeability are also discussed.