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61.
There is a need to develop effective stormwater filters for passive (without any addition of chemicals or energy) and effective removal of pathogens in order to mainstream stormwater harvesting. This study focuses on the development of coated granular activated carbon (GAC) filtration material in order to develop filters for effective removal of pathogens from urban stormwater. Several laboratory trials were performed to gauge the effectiveness of the filters, which use a mixture of the zinc-sulphate-heptahydrate coated GAC and sand, on the removal of Escherichia coli (E. coli) from semi-natural stormwater. On average, a 98% removal of the inflow concentration of E. coli was achieved. Furthermore, there was also an improvement of approximately 25% in the removal of phosphorous. However, it was found that the treated material was leaching zinc. It was important to determine whether the observed removal of E. coli was indirectly caused by the sampling methodology. The results showed that the inactivation of the E. coli in the collected sample was small compared with the inactivation which actually occurred within the filter. This provides much promise to the filter, but the presence of zinc in the outflow demonstrates the need for further investigation into the stabilisation of the coating process. 相似文献
62.
Alves RM Pereira BF Pitol DL Senhorini JA Rocha Rde C Caetano FH 《Microscopy research and technique》2012,75(5):615-619
The adipose fin is small, nonpared, and usually located medially between the dorsal and caudal fin. Its taxonomic occurrence is very restrict; thus, it represents an important trace for taxon distinction. As it does not play a known vital physiological roll and it is easily removed, it is commonly used in marking and recapture studies. The present study characterizes the adipose fin of Prochilodus lineatus, as it is poorly explored by the literature. The adipose fin consists basically of a loose connective core, covered by a stratified epithelium supported by collagen fibers. At the epithelium, pigmented cells and alarm substance cells are found. Despite the name, adipocytes or lipid droplets are not observed on the structure of the fin. 相似文献
63.
Cabral RM Ferraz MS Rizzo M Sousa FD Rodrigues NM Ibiapina PB Ambrósio CE De Carvalho MA 《Microscopy research and technique》2012,75(5):566-570
The aim of this study is to show histological and immunofluorescence analysis of renal parenchyma of agoutis affected by gentamicin‐induced renal disease after the infusion of bone marrow mononuclear cells (BMMC) stained with Hoechst®. Nine agouti's males were divided into three groups: Test group (TG): renal disease by gentamicin induced (n = 3), cell therapy group (CTG): renal disease by gentamicin induced and BMMC infusion (n = 3), and control group (CG): nonrenal disease and BMMC infusion (n = 3). TG and CTG were submitted to the protocol of renal disease induction using weekly application of gentamicin sulfate for 4 months. CG and CTG received a 1 × 108 BMMC stained with Hoechst and were euthanized for kidney examination 21 days after BMMC injection and samples were collected for histology and immunofluorescence analysis. Histological analysis demonstrated typical interstitial lesions in kidney similarly to human disease, as tubular necrosis, glomerular destruction, atrophy tubular, fibrotic areas, and collagen deposition. We conclude that histological analysis suggest a positive application of agouti's as a model for a gentamicin inducing of kidney disease, beyond the immunofluorescence analysis suggest a significant migration of BMMC to sites of renal injury in CTG. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc. 相似文献
64.
After a brief discussion of the algebraic reconstruction techniques (ART), we introduce the attenuation problem in positron emission tomography (PET). We anticipate that a generalization of ART, the so-called cyclic subgradient projection (CSP) method, may be useful for solving this problem. This, however, has not been successfully realized, due to the fact that data collected by our proposed stationary PET detector ring are too sparsely sampled. That this is, in fact, a major problem is demonstrated by showing that ordinary ART produces reconstructions with unacceptably strong artifacts even on perfect (no attenuation) data collected according to the PET geometry. We demonstrate that the source of this artifact is the sparse sampling, and we propose the use of interpolated rays to overcome the problem. This approach is successful, as is illustrated by showing reconstructions from sparsely sampled data by ART with interpolated rays. 相似文献
65.
Streaks arise in computed tomograms for a variety of reasons, such as presence of high-contrast edges and objects, aliasing errors, patient movement, and use of very few views. The problem appears to be an inherent difficulty with all reconstruction methods, including backprojection (with convolution) and the algebraic reconstruction technique (ART). This paper presents the derivation and results of an ART-like algorithm (SPARTAF) oriented towards prevention of streaks via optimization of a cost function based on features of streaks, subject to the constraints of the given projection data. The object-dependent method employs pattern recognition of streaks and adaptive filtering during iterative reconstruction by ART. Results of experiments with a test pattern and of application of the method to reconstructive tomography from radiographic films are presented and the convergence properties demonstrated. 相似文献
66.
Organization of relational models for scene analysis 总被引:1,自引:0,他引:1
Shapiro LG Haralick RM 《IEEE transactions on pattern analysis and machine intelligence》1982,(6):595-602
Relational models are commonly used in scene analysis systems. Most such systems are experimental and deal with only a small number of models. Unknown objects to be analyzed are usually sequentially compared to each model. In this paper, we present some ideas for organizing a large database of relational models. We define a simple relational distance measure, prove it is a metric, and using this measure, describe two organizational/access methods: clustering and binary search trees. We illustrate these methods with a set of randomly generated graphs. 相似文献
67.
Haralick RM Shapiro LG 《IEEE transactions on pattern analysis and machine intelligence》1979,(2):173-184
In this first part of a two-part paper we introduce a general consistent labeling problem based on a unit constraint relation T containing N-tuples of units which constrain one another, and a compatibility relation R containing N-tuples of unit-label pairs specifying which N-tuples of units are compatible with which N-tuples of labels. We show that Latin square puzzles, finding N-ary relations, graph or auto-mata homomorphisms, graph colorings, as well as determining satisfiability of propositional logic statements and solving scene and edge labeling problems, are all special cases of the general consistent labeling problem. We then discuss the various approaches that researchers have used to speed up the tree search required to find consistent labelings. Each of these approaches uses a particular look-ahead operator to help eliminate backtracking in the tree search. Finally, we define the ?KP two-parameter class of look-ahead operators which includes, as special cases, the operators other researchers have used. 相似文献
68.
Despite the great technical advancement of mass spectrometry, this technique has contributed in a limited way to the discovery and quantitation of specific/precocious markers linked to free radical-mediated diseases. Unsaturated aldehydes generated by free radical-induced lipid peroxidation of polyunsaturated fatty acids, and in particular 4-hydroxy-trans-2 nonenal (HNE), are involved in the onset and progression of many pathologies such as cardiovascular (atherosclerosis, long-term complications of diabetes) and neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, and cerebral ischemia). Most of the biological effects of HNE are attributed to the capacity of HNE to react with the nucleophilic sites of proteins and peptides (other than nucleic acids), to form covalently modified biomolecules that can disrupt important cellular functions and induce mutations. By considering the emerging role of HNE in several human diseases, an unequivocal analytical approach as mass spectrometry to detect/elucidate the structure of protein-HNE adducts in biological matrices is strictly needed not only to understand the reaction mechanism of HNE, but also to gain a deeper insight into the pathological role of HNE. This with the aim to provide intermediate diagnostic biomarkers for human diseases. This review sheds focus on the "state-of-the-art" of mass spectrometric applications in the field of HNE-protein adducts characterization, starting from the fundamental early studies and discussing the different MS-based approaches that can provide detailed information on the mechanistic aspects of HNE-protein interaction. In the last decade, the increases in the accessible mass ranges of modern instruments and advances in ionization methods have made possible a fundamental improvement in the analysis of protein-HNE adducts by mass spectrometry, and in particular by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) tandem mass spectrometry. The recent developments and uses of combined analytical approaches to detect and characterize the type/site of interaction have been highlighted, and several other aspects, including sample preparation methodologies, structure elucidation, and data analysis have also been considered. 相似文献
69.
Magnetic particle imaging (MPI) uses safe iron oxide nanoparticle tracers to offer fundamentally new capabilities for medical imaging, in applications as vascular imaging and ultra-sensitive cancer therapeutics. MPI is perhaps the first medical imaging platform to intrinsically exploit nanoscale material properties. MPI tracers contain magnetic nanoparticles whose tunable, size-dependent magnetic properties can be optimized by selecting a particular particle size and narrow size-distribution. In this paper we present experimental MPI measurements acquired using a homemade MPI magnetometer: a zero-dimensional MPI imaging system designed to characterize tracer performance by measuring the derivative of the time-varying tracer magnetization, M'(H(t)), at a driving frequency of 25 kHz. We show that MPI performance is optimized by selecting phase-pure magnetite tracers of a particular size and narrow size distribution; in this work, tracers with 20 nm median diameter, log-normal distribution shape parameter, σ(v), equal to 0.26, and hydrodynamic diameter equal to 30 nm showed the best performance. Furthermore, these optimized MPI tracers show 4?×?greater signal intensity (measured at the third harmonic) and 20% better spatial resolution compared with commercial nanoparticles developed for MRI. 相似文献
70.
Characterization of Spanish honeys with protected designation of origin "Miel de Granada" according to their mineral content 总被引:1,自引:0,他引:1
de Alda-Garcilope C Gallego-Picó A Bravo-Yagüe JC Garcinuño-Martínez RM Fernández-Hernando P 《Food chemistry》2012,135(3):1785-1788
Honey attributes such as geographical origin or specified botanical sources often command a premium price due to their organoleptic or pharmacoactive properties. "Miel de Granada" is a highly quality product with protected designation of origin (PDO) which includes six monofloral honeys and two multifloral honeys. Our objective was the characterization of "Miel de Granada" according to their metal content. Metal content was specific enough and allowed discrimination from honeys of different botanical and geographical origins and confirmed the authenticity of PDO labelling as Granada product with the determination of only five elements (K, Na, Ca, Mg and Zn). Chemometric techniques as cluster analysis and ANOVA were used to classify honeys according to their botanical and geographical origin in the metal data. Metal content marks the differences in honey samples and can be used as a tool to assess the quality of honeys. ANOVA showed significant differences among rosemary honeys from different geographical areas despite the botanical factor weight. Our research contributes to the groundwork studies to determine the geographical origin of Spanish honeys. 相似文献