Near-visible stereolithography of a low shrinkage cationic/free-radical photopolymer blend and its nanocomposite

In this work, a newly prepared cationic/free-radical photopolymer, which consists of two epoxies and a tetrafunctional acrylate, is presented for the first time for a visible light stereolithography (SL), showing the advantages of both cationic and free-radical resins. An onium salt, commonly used as a cationic UV initiator, and a photosensitizer make the blend suitable for a near-visible (405 nm) SL. An increase in the polymerization rate and a drop in the induction period are observed for the newly prepared cationic/free-radical blend, compared with either only cationic systems or free-radical resins. This suggests that the combination of cationic and free-radical polymerizations in a single resin has a positive synergistic effect. The addition of silica nanoparticles to the blend provides a reinforcing and toughening effect. Indeed, the resin loaded with silica shows a 31% increase in the elastic modulus, compared with the unfilled resin. Regarding the values of tensile strength and elongation at break, they, respectively, grow by 47 and 15%, when the nanocomposite resin is compared with the neat resin. A very low volumetric shrinkage of 0.7% and a remarkable printing quality of objects obtained with this new photopolymer will enable the 3D printing of microrobots, bioengineering microdevices, and sensors. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48333.     

Push–Pull Organic Chromophores for Frequency‐Upconverted Lasing

Properly designed organic molecules where a π‐conjugated bridge is end‐capped by an electron‐donor and electron‐withdrawing group (push–pull chromophores) can show, under specific conditions, frequency‐upconverted lasing emission. Materials based on these dyes are therefore able to convert the emission of a cheap and easily available infrared (IR) laser (e.g., 800 nm radiation) into more useful visible (vis) laser emission via a two‐photon absorption induced fluorescence phenomenon. The design of the molecular structure, the substituent effect, and modulation of the electronic and geometric parameters can be used in order to optimize and tune frequency emission and conversion efficiency. Examples of some of the best dyes reported to‐date and organic design strategies employed are discussed in this article.     

A Customizable Instrument for Measuring the Mechanical Properties of Thin Biomedical Membranes

Summary: A customized instrument has been developed as part of multidisciplinary research work relating to the development of a biodegradable vascular scaffold. This instrument aims to measure the mechanical properties of elastic and viscoelastic thin membranes with tissue engineering applications. Uniform and omni‐directional pressure is applied on the whole membrane which is uniformly clamped and submerged into a liquid medium. The mechanical testing described in this study is focused on the stress‐strain curves of polycaprolactone (PCL) films after different treatments. The influence of Dulbecco's modified Eagle's culture medium, L929 fibroblast culture, NaOH treatment and film thickness on the mechanical properties of PCL films was evaluated after different times. These studies show that the PCL degradation process is influenced by immersion in the culture medium, inducing an increment in the slope of the pressure‐dilation curve which is indicative of an increase in the polymer stiffness. On the other hand, long NaOH treatments make PCL films have more flexible behavior.

A computerized version of the instrument: (1) Electrical compressor; (2) Filter; (3) Voltage‐pressure converter; (5) Pressure sensor; (6) Differential pressure sensor; (7–8) Main and auxiliary pipettes; (9) Printed circuit board; (10) Personal computer.     

Therapeutic Acellular Scaffolds for Limiting Left Ventricular Remodelling-Current Status and Future Directions

Myocardial infarction (MI) is one of the leading causes of heart-related deaths worldwide. Following MI, the hypoxic microenvironment triggers apoptosis, disrupts the extracellular matrix and forms a non-functional scar that leads towards adverse left ventricular (LV) remodelling. If left untreated this eventually leads to heart failure. Besides extensive advancement in medical therapy, complete functional recovery is never accomplished, as the heart possesses limited regenerative ability. In recent decades, the focus has shifted towards tissue engineering and regenerative strategies that provide an attractive option to improve cardiac regeneration, limit adverse LV remodelling and restore function in an infarcted heart. Acellular scaffolds possess attractive features that have made them a promising therapeutic candidate. Their application in infarcted areas has been shown to improve LV remodelling and enhance functional recovery in post-MI hearts. This review will summarise the updates on acellular scaffolds developed and tested in pre-clinical and clinical scenarios in the past five years with a focus on their ability to overcome damage caused by MI. It will also describe how acellular scaffolds alone or in combination with biomolecules have been employed for MI treatment. A better understanding of acellular scaffolds potentialities may guide the development of customised and optimised therapeutic strategies for MI treatment.     

The translation initiation factor eIF4A from Schizosaccharomyces pombe is closely related to its mammalian counterpart

We have isolated a cDNA clone encoding eIF4A from Schizosaccharomyces pombe. The deduced protein sequence is similar in length and sequence to other eIF4A proteins and exhibits highest similarity with the mammalian eIF4A protein. Hybridization with genomic DNA reveals two eIF4A genes located on two different chromosomes. This sequence has been deposited in the EMBL Data Library under Accession Number X80796.     

Robust frame synchronization for the DVB‐S2 system with large frequency offsets

This paper tackles the problem of frame synchronization design for the Digital Video Broadcasting standard via Satellite (DVB‐S2) system. In particular, the design of a novel frame detection scheme exhibiting robustness against large frequency errors is proposed. Post‐detection integration (PDI) is exploited to provide coarse frame alignment with limited complexity with respect to classical approximate maximum likelihood (ML) solutions. The novel scheme, denoted as differential generalized post‐detection integration, derives from a pragmatic adaptation of the well‐known generalized PDI scheme (IEEE Trans. Commun. 2007; 55 (11):2159–2171), which eliminates its inherent source of weakness in the presence of large frequency offsets, i.e. its non‐differential component given by non‐coherent PDI. The proposed approach manages to reduce the remarkable performance gap with respect to approximate ML techniques available in the literature (IEEE Trans. Commun. 2002; 50 (7):1062–1065), providing the designer with an interesting performance/complexity trade‐off. Performance is numerically evaluated in terms of receiver operating characteristics and mean acquisition time (MAT), handling the acquisition procedure according to a single‐dwell approach. Both threshold crossing and MAX criterion are contrasted to identify the best design solution. Copyright © 2008 John Wiley & Sons, Ltd.     

Abbreviated kinetic profiles in area-under-the-curve monitoring of cyclosporine therapy. Technical note

BACKGROUND: The new microemulsion formulation of cyclosporine (CsA-ME) displays more consistent pharmacokinetic properties than the original formulation and may allow successful implementation of an abbreviated area-under-the-curve (AUC) strategy. METHODS: Here we compared two limited sampling strategies in order to define the one that best predicts AUC after CsA-ME in 51 renal transplant recipients with stable renal function. Pharmacokinetics were based on analysis of blood samples collected over 12 hours after drug administration by high-performance liquid chromatography (HPLC). Predicted AUC was estimated by using a three-point (0, 1 and 3 hr) or a two-point (2 and 6 hr or 0 and 2 hr) sampling strategy. RESULTS: A simplified strategy with three time points of blood collection at 0, 1, and 3 hours after CsA-ME allowed adequate and accurate prediction of the daily exposure to CsA. AUC prediction with two-point sampling at 2 and 6 hours was less good with a very large error in prediction (only 59% of the estimated AUC were within the accepted range). This limitation was even more evident when the 0 and 2 hour time points were examined, in which only 51% of AUC estimates were included in the accepted range of variation (-10 to 10%). CONCLUSIONS: A limited strategy of three-point sampling taken early after dosing allows an excellent and perfectly reliable prediction of the actual AUC.     

Automated classification of non-functional requirements

This paper describes a technique for automating the detection and classification of non-functional requirements related to properties such as security, performance, and usability. Early detection of non-functional requirements enables them to be incorporated into the initial architectural design instead of being refactored in at a later date. The approach is used to detect and classify stakeholders’ quality concerns across requirements specifications containing scattered and non-categorized requirements, and also across freeform documents such as meeting minutes, interview notes, and memos. This paper first describes the classification algorithm and then evaluates its effectiveness through reporting a series of experiments based on 30 requirements specifications developed as term projects by MS students at DePaul University. A new and iterative approach is then introduced for training or retraining a classifier to detect and classify non-functional requirements (NFR) in datasets dissimilar to the initial training sets. This approach is evaluated against a large free-form requirements document obtained from Siemens Logistics and Automotive Organization. Although to the NFR classifier is unable to detect all of the NFRs, it is useful for supporting an analyst in the error-prone task of manually discovering NFRs, and furthermore can be used to quickly analyse large and complex documents in order to search for NFRs.