Heat-induced Maillard reaction of the tripeptide IPP and ribose: Structural characterization and implication on bioactivity

Maillard reaction products (MRPs) were prepared from aqueous model mixtures containing 60 g L^? 1 ribose and 30 g L^? 1 of the bioactive tripeptide IPP (Ile-Pro-Pro), heated at 98 °C. MRP and associated reactions with changes in IPP were observed within one hour of heat-treatment. The pH of MRPs decreased significantly during the heat treatment of IPP–ribose mixtures from 9.0 to 7.6 after one hour. The amino group content, IPP and ribose concentration decreased significantly during heat treatment. The fluorescence intensity of the IPP–ribose MRPs reached the maximum within 2 h. Modification of the UV/vis spectra for IPP–ribose MRPs was mainly due to a condensation reaction of IPP with ribose. Compounds with molecular weight between 300 and 650 Da were dominant while compounds smaller than 250 Da were also produced during the reactions, as characterized by size exclusion chromatography. Mass spectrometry revealed that IPP was conjugated to ribose at the N-terminal (m/z of 458.3) upon heat-treatment. The presence of ribose also promoted peptide degradation to dehydrated IP (m/z of 211.1). IPP–ribose MRPs lost the known angiotensin-I-converting enzyme (ACE) inhibitory activity of IPP; however, strong antioxidant properties were detected.     

A computer approach for reliability evaluation of telecommunicationnetworks with heterogeneous link-capacities

The authors present a computer approach to obtaining a survivability index called capacity related reliability (CRR) in large telecommunication networks where links have different capacities. The method is a two-step approach. The first step deals with composite path enumeration (CPE). A k-composite path is defined as the union of the set of edges in any k simple paths and relates link capacity and network connectivity. The CPE approach is an improvement over the algorithms proposed by the authors earlier (1991). In step two, k-composite paths information is manipulated to generate the CRR. The authors use CAREL (computer aided reliability evaluator) to solve this step. The technique is automated using C on the Encore Multimax System. The results on CRR for three networks with various values of minimum message capacity are presented. An exhaustive technique is used to verify these results. An informal proof of the CPE approach is also included     

Studies in surface chemistry of carbon blacks-vi. adsorption isotherms of benzene on carbons associated with different surface oxygen complexes

Adsorption isotherms of benzene (35°C) on carbon blacks are of Type II and show complete reversibility. The presence of CO₂-complex, which imparts polar and hydrophilic character to the surface, suppresses the sorption of benzene but its elimination and the emergence of CO-complex as the only predominant complex enhances the sorption at all r.v.p's. The additional sorption amounts roughly to one molecule of benzene per quinonic oxygen as obtained by sodium borohydride treatment. This indicates probability of interaction of π electrons of benzene ring with partial positive charge on carbonyl carbon atom. The isosteric heat of adsorption is also higher in the presence of quinonic oxygen. The use of benzene isotherms for estimating surface area or pore volume of carbons may, therefore, be looked upon with caution. The isotherm on sugar charcoal also shifts bodily upward with the emergence of CO-complex but the adsorbed benzene cannot be recovered even on prolonged evacuation, showing that the benzene complexed at the quinonic sites within the microporous system requires activation energy to diffuse out of the pores. The interaction of benzene, due to the presence of π electrons, seems to be specific, since the isotherms of cyclohexane on a given carbon black are identical irrespective of the nature or amount of any of the surface oxygen complexes present in it.     

Dependability Under Priority Repair Disciplines

We have studied the operational behaviour of a complex system consisting of two classes of units (with standby redundancy in one class) under two repair disciplines, viz., preemptive-resume and preemptive-repeat. Units have constant failure rate, while repair follows general probability distributions. Supplementary variable and Laplace transform techniques have been used to obtain the transient state probabilities for such a system. From these, steady state behaviour of the complex system has been examined.     

Methods for calculating point-to-point congestion in switched communication networks

This paper presents two simple algorithms for computing the point-to-point congestion probability in a switched communication network. Methods are straightforward and can easily be computerized. Examples illustrate the methods.     

On Lifetime-Based Node Failure and Stochastic Resilience of Decentralized Peer-to-Peer Networks

To model P2P networks that are commonly faced with high rates of churn and random departure decisions by end-users, this paper investigates the resilience of random graphs to lifetime-based node failure and derives the expected delay before a user is forcefully isolated from the graph and the probability that this occurs within his/her lifetime. Using these metrics, we show that systems with heavy-tailed lifetime distributions are more resilient than those with light-tailed (e.g., exponential) distributions and that for a given average degree, k-regular graphs exhibit the highest level of fault tolerance. As a practical illustration of our results, each user in a system with n = 100 billion peers, 30-minute average lifetime, and 1-minute node-replacement delay can stay connected to the graph with probability 1 - 1/n using only 9 neighbors. This is in contrast to 37 neighbors required under previous modeling efforts. We finish the paper by observing that many P2P networks are almost surely (i.e., with probability 1 - o(1)) connected if they have no isolated nodes and derive a simple model for the probability that a P2P system partitions under churn.     

Photocatalytic degradation of indigo carmine dye using TiO<Subscript>2</Subscript> impregnated activated carbon

The photocatalytic degradation of indigo carmine dye was studied using hydrothermally prepared TiO₂ impregnated activated carbon (TiO₂: AC). A comparison between the degradation of the indigo carmine dye using commercial TiO₂ and TiO₂: AC revealed the efficiency of the title compound. The degradation reaction was optimized with respect to the dye concentration and catalyst amount. The reduction in the chemical oxygen demand (COD) revealed the mineralization of dye along with colour removal. The active compound like TiO₂ was impregnated onto the activated carbon surface under mild hydrothermal conditions (<250°C, P ∼ 40 bars). The impregnated activated carbon samples were characterized using powder X-ray diffraction (XRD) and scanning electron microscope (SEM).     

Annual and seasonal variations in tropospheric ozone concentrations around Varanasi

This study examines the annual, seasonal and diurnal variations in the ambient concentrations of ozone at a suburban site of Varanasi, India, during 2002–2006. Prominent seasonal variations in ozone concentrations were recorded. Ozone concentrations were higher during the warmer months. Daytime 12‐hourly mean monthly ozone concentrations varied from 45.18 to 62.35 ppb during summer, from 28.55 to 44.25 ppb during winter and from 24 to 43.85 ppb during the rainy season from 2002 to 2006. Distinct diurnal variations in ozone concentrations were also observed. Daytime maxima in ozone concentration were recorded between 1200 and 1400 h, whereas morning and evening hours showed lower concentrations of ozone. Ozone concentrations in the atmosphere depended on several meteorological factors. Monthly average ozone concentration was significantly correlated with maximum temperature (p<0.001) and mean monthly temperature (p<0.05), maximum relative humidity (p<0.001), minimum relative humidity (p<0.001) and mean monthly relative humidity (p<0.001), and sunshine hours (p<0.001). Ozone concentrations in the ambient air have shown an increase in the past decade that was more in the winter and rainy seasons than in the summer. This study suggests that ozone concentrations around Varanasi were sufficiently high to cause significant damage to agricultural production. The present work can be extended to a regional level by incorporating modelling studies using recent remote sensing tools.     

Prediction of semiconducting behavior in minority spin of Co2CrZ（Z=Ga,Ge,As）:LSDA

正Volume optimization was performed to obtain the theoretical lattice constants by using the generalized gradient approximation(GGA).The electronic and magnetic properties of Heusler alloys Co_2CrZ(Z = Ga,Ge, As) were investigated by using local spin density approximation(LSDA).Amongst the systems under investigation, Co_2CrGe and Co_2CrGa give 100%spin polarization at the Fermi level(E_f)- Co_2CrGe and Co_2CrGa are the most stable half-metallic ferromagnets(HMFs);their E_F lie exactly at the gap of 0.24 eV and 0.38 eV,respectively,in the spin-down channel.Even though Co_2CrAs gives a distinct and bigger gap as compared to Co_2CrGa and Co_2CrGe, its E_F is not located at the middle of the gap in the spin-down channel.We have also found that the total magnetic moments increase as the Z goes from Ga to As.The calculated density of states and band structures show the HMF character for Co_2CrGe and Co_2CrGa.     

Isolation of directed evolution mutants of chloroperoxidase resistant to suicide inactivation by primary olefins

Wild-type chloroperoxidase catalyzes the efficient chiral epoxidation of secondary olefins but is rapidly inactivated in a mechanism-based suicide reaction when incubated with hydrogen peroxide and primary olefins. Directed evolution mutants of chloroperoxidase have now been isolated that are resistant to suicidal inactivation. Plasmid vectors containing error-prone copies of the chloroperoxidase gene and a hygromycin B resistance marker gene have been used to transform Caldariomyces fumago spheroplasts and produce mutant libraries. The mutant library clones were screened for their ability to resist mechanism-based inactivation by allylbenzene. Four generations of PCR-based random mutagenesis and screening yielded mutants that were completely resistant to the suicide-inactivation reaction. Rather surprisingly, the fourth generation mutant developed enhanced epoxidation activity in addition to resistance to allylbenzene inactivation. These initial results suggest that the directed evolution technique can be used to produce chloroperoxidase variants that can further exploit the potential of chloroperoxidase for the synthesis of chiral intermediates.