Oxidant damage to erythrocyte membrane in glucose-6-phosphate dehydrogenase deficiency: correlation with in vivo reduced glutathione concentration and membrane protein oxidation

Chronic nonspherocytic hemolytic anemia has been observed in a recently described glucose-6-phosphate dehydrogenase (G6PD) variant, G6PDWayne. The mechanical properties of these erythrocytes and other G6PD variants were examined. The deformability of G6PD-deficient erythrocytes was normal, as determined by osmotic scan ektacytometry, and was not significantly affected by hemolytic crisis. In the common varieties of G6PD deficiency, the mechanical stability of the red blood cell (RBC) membrane was greater than normal, but G6PDWayne membranes were abnormally susceptible to shear-induced fragmentation. There was no evidence for a concurrent genetic defect in spectrin, because self-association constants and tryptic digests were normal. The fragility of G6PDWayne membranes appeared to be a consequence of oxidative damage to membrane thiol groups associated with a low glutathione (GSH) level in these RBCs. Associations among GSH level, thiol oxidation, and membrane instability were also found when a larger group of G6PD-deficient RBCs were examined. In normal erythrocytes, 1-chloro-2,4-dinitrobenzene was used to reduce GSH levels by 50%. Membrane thiol oxidation and membrane fragility both increased when these cells were kept at 4 degrees C for 3 to 5 days. Our findings suggest that chronic depletion of GSH leads to the destabilization of membrane skeleton through oxidation of membrane protein thiols.     

Über die Autoxydation von Bicyclo[2,2,2]octen

On the Autoxidation of Bicyclo[2,2,2]octene During the autoxidation of bicyclo[2,2,2]octene at 90°C about 50% of epoxide are obtained. The most important byproduct obviously is a polymeric peroxide. From the acidic products formed in low amounts, cis-1, 4-Cyclohexane dicarboxylic acid could be isolated as the corresponding dimethyl ester.     

Modeling of poly(ethylene terephthalate) reactors. IX. Solid state polycondensation process

A mathematical model for simulation of industrial process of solid state polycondensation of poly(ethylene terephthalate) (PET) has been developed. The model eliminates errors evident in the earlier models by proper formulation. The model results have been validated by experimental data in the literature. It enables prediction of the influence of particle shape, size, temperature, etc. on the polycondensation process correctly in all different regimes of operation, apart from bringing out the importance of gas-side resistance, influence of carrier gas, etc. for the first time.     

Facile Fabrication and Superparamagnetism of Silica‐Shielded Magnetite Nanoparticles on Carbon Nitride Nanotubes

Using conventional methods to synthesize magnetic nanoparticles (NPs) with uniform size is a challenging task. Moreover, the degradation of magnetic NPs is an obstacle to practical applications. The fabrication of silica‐shielded magnetite NPs on carbon nitride nanotubes (CNNTs) provides a possible route to overcome these problems. While the nitrogen atoms of CNNTs provide selective nucleation sites for NPs of a particular size, the silica layer protects the NPs from oxidation. The morphology and crystal structure of NP–CNNT hybrid material is investigated by transmission electron microscopy (TEM) and X‐ray diffraction. In addition, the atomic nature of the N atoms in the NP–CNNT system is studied by near‐edge X‐ray absorption fine structure spectroscopy (nitrogen K‐edge) and calculations of the partial density of states based on first principles. The structure of the silica‐shielded NP–CNNT system is analyzed by TEM and energy dispersive X‐ray spectroscopy mapping, and their magnetism is measured by vibrating sample and superconducting quantum interference device magnetometers. The silica shielding helps maintain the superparamagnetism of the NPs; without the silica layer, the magnetic properties of NP–CNNT materials significantly degrade over time.     

Amphiphilic Poly(p‐phenylene)s for Self‐Organized Porous Blue‐Light‐Emitting Thin Films

Micro‐ and nanostructuring of conjugated polymers are of critical importance in the fabrication of molecular electronic devices as well as photonic and bandgap materials. The present report delineates the single‐step self‐organization of highly ordered structures of functionalized poly(p‐phenylene)s without the aid of either a controlled environment or expensive fabrication methodologies. Microporous films of these polymers, with a honeycomb pattern, were prepared by direct spreading of the dilute polymer solution on various substrates, such as glass, quartz, silicon wafer, indium tin oxide, gold‐coated mica, and water, under ambient conditions. The polymeric film obtained from C₁₂PPPOH comprises highly periodic, defect‐free structures with blue‐light‐emitting properties. It is expected that such microstructured, conjugated polymeric films will have interesting applications in photonic and optoelectronic devices. The ability of the polymer to template the facile micropatterning of nanomaterials gives rise to hybrid films with very good spatial dispersion of the carbon nanotubes.     

Development of microbiologically safe mung bean sprouts using combination treatment of sodium hypochlorite and gamma radiation

Specific group of people, with impaired immune system, are recommended to consume pathogen‐free foods. In this study, microbiologically safe ready‐to‐eat (RTE) mung bean sprouts were developed using combination treatment (CT) with 200 ppm sodium hypochlorite and 12 kGy dose of gamma radiation. Microbiological analysis of combination‐treated sprout samples showed complete elimination (<10 CFU g^?1 of sprouts) of microbial load in these samples, even during storage at 4 °C up to 12 days. Combination treatment and storage period did not have any significant effect on the sensory qualities of RTE mung bean sprouts. However, reduction in the firmness and vitamin C content of combination‐treated sprout samples, similar to other food processing methods, was observed. These results suggest that CT is effective in sterilisation of mung bean sprouts. These sprouts can be included in the diets of special target groups and thereby improve in their quality of life.     

Membrane application for recovery and reuse of water from treated tannery wastewater

Secondary treated tannery wastewater contains high concentrations of Total Dissolved Solids (TDS) and other residual organic impurities, which cannot be removed by conventional treatment method. A pilot plant membrane system with a designed processing capacity of 1 m³/h, comprising of nano and reverse osmosis (RO) membrane units, accompanied by several pre-treatment operations, was evaluated in order to further treat and reuse the tannery wastewater. The maximum TD S removal efficiency of the polyamide RO membrane was more than 98%. The permeate recovery of about 78% was achieved. The water recovered from the membrane system, which had very low TDS concentration, was reused for wet finishing process in the tanneries. The reject concentrate obtained from the operation was sent to solar evaporation pans. It was evident from the study that the membrane system can successfully be applied for recovery of water from secondary treated tannery effluent, provided a suitable and effective pretreatment system prior to membrane system is employed. Combining nano and RO membranes improved the life of the membranes and permeate recovery rate.     

Phase‐dependent radiation‐resistant behavior of BaTiO3: An in situ X‐ray diffraction study

The radiation‐resistant response of BaTiO₃ in the tetragonal and rhombohedral phases on exposure to 100 MeV Ag⁷⁺ ion irradiation was investigated by in situ X‐ray diffraction (XRD) at room temperature (300 K) and low temperature (25 K), respectively. This study revealed that the BaTiO₃ in rhombohedral phase retained crystallinity up to an ion fluence of 1×10¹⁴ ions/cm², whereas tetragonal phase amorphized at much lower fluence viz. 1×10¹³ ions/cm². The in situ XRD along with Raman spectroscopy studies revealed that BaTiO₃ in rhombohedral phase is more radiation resistant than that of tetragonal phase. The density functional theory (DFT) calculations confirmed higher bond strength of rhombohedral phase as compared to tetragonal phase, which supported the experimental result of higher radiation stability of rhombohedral phase. The theoretical predictions on high‐temperature phase will be of relevance to the nuclear waste applications.     

A Novel Low-Temperature Synthesis of Nanosized NiZn Ferrite

Nanosized NiZn ferrite powder is synthesized by a low-temperature method, using a unique combination of citric acid and glycine. An appropriate molar ratio of both citric acid and glycine offers a low-temperature synthetic route by incorporating the complexation behavior of citric acid and the combustion nature of glycine. Thermal decomposition/controlled autocatalytic combustion of the composite gel occurs at a low temperature of around 175°C, with the evolution of a large amount of gases. Transmission electron microscopic studies showed that the average particle size of the ferrite obtained is ∼2.5 nm, with a narrow size distribution. Uniformly distributed fine-grained microstructure with low porosity is obtained for a sample sintered at 1000°C.     

Formation of BaCrO4 NanoCrystallites within Thermally Evaporated Sodium Bis-2-Ethylhexyl-Sulfosuccinate and Stearic Acid Thin Films

This paper describes the growth of barium chromate (BaCrO₄) nanocrystallites within thermally evaporated thin films of stearic acid (StA) and sodium bis-2-ethylhexyl-sulfosuccinate by a process of Ba²⁺ ion entrapment followed by in situ reaction with CrO₄²⁻ ions. Dense spherical assemblies of BaCrO₄ nanocrystallites of very uniform size (∼50 nm) were obtained within the two different host matrices. The spherical assemblies were composed of smaller (ca. 5–10 nm size) BaCrO₄ crystals indicating that efficient size control over crystal size may be exercised by the matrix. Contact angle measurements of the BaCrO₄–StA and BaCrO₄–sodium bis-2-ethylhexyl-sulfosuccinate films indicated that they were hydrophobic, thus pointing to the possible role of hydrophobic interaction between the StA and sodium bis-2-ethylhexyl-sulfosuccinate monolayer-covered BaCrO₄ crystals in the assembly process.