颁奖典礼我主持——分屏特效的制作

看过奥斯卡颁奖典礼的朋友或许都会对颁奖前司仪或是嘉宾宣布奖项的画面转播方式记忆犹新——四个候选人将画面一分为四，每个画面上的候选者表情各异，等最终结果出现后，其中一个角色的画面增大覆盖掉其他三个画面并占据整个屏幕，这种类似于电视墙的效果，其实我们用Premiere即可轻松完成，不信？那就快跟我来吧。     

让字体飞起来

在上期的“梦工厂”栏目里．我们介绍了灯光效果片头的制作方法。不少读者纷纷来信．表示对该类文章的兴趣．并要求介绍更多的特效制作。别急．在这样一个讲求个性的年代里．Premiere又怎会让你失望呢？今天就让笔者为大家介绍一种新的片头字幕制作方法——飞射字。     

Evaluation of a 7‐Methoxycoumarin‐3‐carboxylic Acid Ester Derivative as a Fluorescent,Cell‐Cleavable,Phosphonate Protecting Group

Cell‐cleavable protecting groups often enhance cellular delivery of species that are charged at physiological pH. Although several phosphonate protecting groups have achieved clinical success, it remains difficult to use these prodrugs in live cells to clarify biological mechanisms. Here, we present a strategy that uses a 7‐methoxycoumarin‐3‐carboxylic acid ester as a fluorescent protecting group. This strategy was applied to synthesis of an (E)‐4‐hydroxy‐3‐methyl‐but‐2‐enyl diphosphate (HMBPP) analogue to assess cellular uptake and human Vγ9Vδ2 T cell activation. The fluorescent ester displayed low cellular toxicity (IC₅₀>100 μm ) and strong T cell activation (EC₅₀=0.018 μm ) relative to the unprotected anion (EC₅₀=23 μm ). The coumarin‐derived analogue allowed no‐wash analysis of biological deprotection, which revealed rapid internalization of the prodrug. These results demonstrate that fluorescent groups can be applied both as functional drug delivery tools and useful biological probes of drug uptake.     

A modified allomone collecting apparatus

A modified allomone collecting apparatus was designed that could be used under water with a scuba tank. This apparatus provides a simple method of obtaining naturally secreted chemicals from benthic marine organisms at scuba depths, for the first time without the necessity of using a bilge pump. Organic material from Sep-paks in the allomone collector confirmed the release of secondary metabolites from a soft coral into the surrounding water.     

可扩展的多目标最优化多播路由

提出了一种提高多播可扩展性的新思路——将多播可扩展性作为一个最优化目标引入到多播路由算法的设计中,采用多目标最优化路由算法,提高现有多播可扩展性方法的效率.采用多目标最优化路由设计方法对AM(aggregated multicasc)和DTM(dynamic tunnel multicast)两种方法进行改进,给出了相应的最优化目标、启发式多目标最优化多播路由算法和多目标最优化多播路由遗传算法.对于AM方法,使用该算法可以有效地减少汇聚多播树的数量;对于DTM方法,使用该算法可以有效地增加非分枝节点的数量,减少多播状态.     

灵光一闪，够酷！——无厘头效果轻松做

喜欢漫画的朋友肯定熟悉这样的情节——当漫画人物恶念顿生或灵光闪现的时候，他们的眼角或嘴角处都会有白光一闪（有时为了表现少女的楚楚可怜或是男主角的机智果敢，眼角处也都加入闪烁的亮光）。你是否想让自己的照片或者视频片段也加入这种无厘头效果？那还不简单？赶快打开Photoshop和Premiere吧！     

靓丽大都市 潮流美女最爱之选我有我的EeePC

时间临近6月，街头已是一片初夏光景。鲜花伴绿叶，娇嫩更加。更有穿梭在大街之上的形形色色美女，无一不用她们姣好的面容、青春的气息，撩拨着宅男们的心房。不经意间，发现她们手中的数码产品又换了样，从以前的MP3、PSP，变成了现在的“小本本”。     

吓死人不偿命——巧用Premiere制作镜中影像

寂静的午夜，一位略显清便的白农少女对着镜子经缓也梳理着自己的头发，谁知道镜中却慢慢出现了另外一张陌生的面孔……各位稳住！别怕！这不过是恐怖电影里的一个常用桥段而已，在Rremiare中，我们也可以做出类似的效果，下面我们就来开始制作这部精彩的灵异片段吧。     

Selective Inhibitors of Glutathione Transferase P1 with Trioxane Structure as Anticancer Agents

The response to chemotherapy in cancer patients is frequently compromised by drug resistance. Although chemoresistance is a multifactorial phenomenon, many studies have demonstrated that altered drug metabolism through the expression of phase II conjugating enzymes, including glutathione transferases (GSTs), in tumor cells can be directly correlated with resistance against a wide range of marketed anticancer drugs. In particular, overexpression of glutathione transferase P1 (GSTP1) appears to be a factor for poor prognosis during cancer therapy. Former and ongoing clinical trials have confirmed GSTP1 inhibition as a principle for antitumor therapy. A new series of 1,2,4‐trioxane GSTP1 inhibitors were designed via a type II photooxygenation route of allylic alcohols followed by acid‐catalyzed peroxyacetalization with aldehydes. A set of novel inhibitors exhibit low micromolar to high nanomolar inhibition of GSTP1, revealing preliminary SAR for further lead optimization. Importantly, high selectivity over another two human GST classes (GSTA1 and GSTM2) has been achieved. The trioxane GSTP1 inhibitors may therefore serve as a basis for the development of novel drug candidates in overcoming chemoresistance.