Purification and Properties of β-Glucuronidase from the Muscle of Bombay Duck (Harpodon Nehereus)

Bombay duck muscle β-glucuronidase purified 4200-fold had molecular weight of 160,000 as estimated by gel filtration on Sephadex G-200. The glycoprotein enzyme exhibited dimeric structure on SDS-PAGE and had a pI of 5.0. The enzyme was optimally active at pH 5.2 when phenolphthalein β-D-glucuronide was used as the substrate while with p-nitrophenyl glucuronide the pH optimum was 4.6. Saccharo-1,4-lactone was a potent competitive inhibitor of the enzyme. Heavy metalic ions such as Hg²⁺, Cd²⁺ and Ag⁺ also proved to be inhibitory to the enzyme. Radiation inactivation of the enzyme could be protected in the presence of mercaptoethanol. Sodium chloride activated the enzyme while sodium tripolyphosphate inhibited it.     

Biocides used in building materials and their leaching behavior to sewer systems.

There is increasing concern about diffuse pollution of aquatic systems by biocides used in urban areas. We investigated sources and pathways of biocides significant for the pollution of storm water runoff. Main sources seem to be building envelopes, i.e. facades (paints, plasters) and roof sealing membranes. First results from a defined urban catchment drained by a separated sewer system without any agricultural activities reveal a substantial occurrence. Even after the first flush, concentrations of terbutryn, carbendazim, mecoprop as well as Irgarol 1051 and its metabolite exceeded the Swiss water quality standard of 0.1 microg/L. In laboratory experiments, leaching of mecoprop used as a root protection agent in bitumen sheets for roof waterproofing was determined. The concentrations differed in 16 different sheets two orders of magnitude, depending on the product composition. Using optimized products, it is expected to be the most efficient and sustainable way to reduce the environmental impact. To understand transport dynamics and environmental risk, further storm water events will be analyzed. Based on the ongoing project URBIC, first measures will be proposed to limit the release to surface and ground water.     

SED88: a Pascal program for the analysis of sedimentation equilibrium data

Analytical ultracentrifugation is commonly used for the determination of molecular weights (sedimentation equilibrium) and sedimentation coefficients (sedimentation rate) of biological macromolecules in solution. A Turbo Pascal program for the analysis of sedimentation equilibrium centrifugation data produced by absorbance optical systems is described. The user may enter data from a scan of absorbance versus distance from the centre of rotation, via a graphics tablet (or ASCII file). This is subsequently manipulated to yield an apparent weight average molecular weight for the given sample. Plots of ln (absorbance) versus (radius2) may also be produced. The method described uses readily available computational equipment requiring only a graphics tablet in addition to an IBM PC compatible computer. This technique and the software developed have been used to investigate the molecular weight range of two International Humic Substances Society (IHSS) reference samples from the Suwannee River.     

Decentralized energy supply: Suitability for the UK's industrial sectors

From the results reported here it is suggested that policy decisions about the potential contribution of decentralized energy supply systems to UK industrial energy requirements should be based on the suitability of individual sectors rather than averaged over all sectors. The suitability of decentralized energy sources is evaluated, based on a themodynamic match between source and demand. This suitability criterion is used to identify the most appropriate sector in manufacturing industries for the adoption of decentralized energy supply, leaving aside economic cost considerations and individual site requirements.