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141.
Reliable joints of Ti3SiC2 ceramic and TC11 alloy were diffusion bonded with a 50 μm thick Cu interlayer. The typical interfacial structure of the diffusion boned joint, which was dependent on the interdiffusion and chemical reactions between Al, Si and Ti atoms from the base materials and Cu interlayer, was TC11/α-Ti + β-Ti + Ti2Cu + TiCu/Ti5Si4 + TiSiCu/Cu(s, s)/Ti3SiC2. The influence of bonding temperature and time on the interfacial structure and mechanical properties of Ti3SiC2/Cu/TC11 joint was analyzed. With the increase of bonding temperature and time, the joint shear strength was gradually increased due to enhanced atomic diffusion. However, the thickness of Ti5Si4 and TiSiCu layers with high microhardness increased for a long holding time, resulting in the reduction of bonding strength. The maximum shear strength of 251 ± 6 MPa was obtained for the joint diffusion bonded at 850 °C for 60 min, and fracture primarily occurred at the diffusion layer adjacent to the Ti3SiC2 substrate. This work provided an economical and convenient solution for broadening the engineering application of Ti3SiC2 ceramic. 相似文献
142.
Dr. Sandra Liebscher Dr. Sebastian Mathea Dr. Tobias Aumüller Dr. Andreas Pech Prof. Dr. Frank Bordusa 《Chembiochem : a European journal of chemical biology》2021,22(7):1201-1204
Fluorescent fusion proteins are powerful tools for studying biological processes in living cells, but universal application is limited due to the voluminous size of those tags, which might have an impact on the folding, localization or even the biological function of the target protein. The designed biocatalyst trypsiligase enables site-directed linkage of small-sized fluorescence dyes on the N terminus of integral target proteins located in the outer membrane of living cells through a stable native peptide bond. The function of the approach was tested by using the examples of covalent derivatization of the transmembrane proteins CD147 as well as the EGF receptor, both presented on human HeLa cells. Specific trypsiligase recognition of the site of linkage was mediated by the dipeptide sequence Arg-His added to the proteins’ native N termini, pointing outside the cell membrane. The labeling procedure takes only about 5 minutes, as demonstrated for couplings of the fluorescence dye tetramethyl rhodamine and the affinity label biotin as well. 相似文献
143.
144.
目的:研究抗坏血酸(ascorbic acid,AsA)-还原型谷胱甘肽(reduced glutathione,GSH)循环代谢在水杨酸处理采后甜瓜诱导的过量H2O2清除过程中的作用。方法:用4 mmol/L水杨酸浸泡‘玉金香’厚皮甜瓜10 min,测定处理后果实常温贮藏过程中丙二醛(malondialdehyde,MDA)含量,分析活性氧的积累水平、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活力,以及AsA-GSH循环过程相关酶活力及产物和底物含量。结果:水杨酸处理降低了果实MDA含量,第10天处理组MDA含量较对照组降低14.6%;显著提高了果实O2-·的产生速率和H2O2含量(P<0.05),其中处理后第2天O2-·的产生速率高出同期对照组的1.9 倍,第6天H2O2含量高出对照组果实29.7%;提高了果实SOD活力,但抑制了CAT活力,说明H2O2的清除可能是依赖于除酶促系统外的其他系统。此外,水杨酸处理提高了果实抗坏血酸过氧化物酶、单脱氢抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶的活力,增加了AsA和氧化型谷胱甘肽的含量,降低了脱氢抗坏血酸和GSH的含量。结论:水杨酸处理诱导了厚皮甜瓜果实的氧爆,抑制了MDA产生,由水杨酸诱导产生的过量H2O2主要依靠AsA-GSH循环系统清除。 相似文献
145.
Changbai Long Ning Ji Li Yang Wenjie Zhou Kun Zheng Wei Ren Laijun Liu 《Journal of the European Ceramic Society》2021,41(6):3369-3378
With co-substitution of (Li0.5Sm0.5) at A site and W at B site, the electrical properties of modified Ca0.92(Li0.5Sm0.5)0.08Bi2Nb2-xWxO9 [(CLS)BN-xW, x = 0, 0.015 and 0.03] piezoceramics with ultrahigh Curie temperature (TC) of > 930 °C were enhanced dramatically. The increased resistivity induced by the co-substitution ensure them to be polarized under an enough high field. Combined with the increase of spontaneous ferroelectric polarization (PS), the significant enhancements in the piezoelectric, dielectric and ferroelectric properties can be obtained in the composition x = 0.015. Furthermore, the piezoelectric activity (d33) and bulk resistivity (ρb) of (CLS)BN-0.015 W can be further enhanced at an appropriate sintering temperature. This optimum composition sintered at 1170 °C shows ultrahigh TC of ~948 °C, d33 of ~17.3 pC/N and ρb of ~6.9 MΩ cm at 600 °C, which are comparable to those of the reported high-temperature Aurivillius piezoceramics with TC > 850 °C. 相似文献
146.
Castro Mateus B. Souza Raphael R. N. Junior Agord M. P. Lima Eduardo R. Manera Leandro T. 《Analog Integrated Circuits and Signal Processing》2021,109(3):647-656
Analog Integrated Circuits and Signal Processing - This paper presents the complete design of a phase locked loop-based clock synthesizer for reconfigurable analog-to-digital converters. The... 相似文献
147.
Zhang Qi Wang Yujing Zhang Xueling Song Jun Li Yinlei Wu Xuehong Yuan Kunjie 《Journal of Materials Science》2022,57(14):7208-7224
Journal of Materials Science - Form-stable composite phase change materials (C-PCMs) prepared by microencapsulation method and porous matrix adsorption method need for compression molding after... 相似文献
148.
149.
Catalysis Letters - An environmentally benign process for synthesizing 4-methoxyphenol through methylation of hydroquinone using polystyrene immobilized Bronsted acidic ionic liquid is presented.... 相似文献