Conversion of 3-chlorocatechol by various catechol 2,3-dioxygenases and sequence analysis of the chlorocatechol dioxygenase region of Pseudomonas putida GJ31

Pseudomonas putida GJ31 contains an unusual catechol 2,3-dioxygenase that converts 3-chlorocatechol and 3-methylcatechol, which enables the organism to use both chloroaromatics and methylaromatics for growth. A 3.1-kb region of genomic DNA of strain GJ31 containing the gene for this chlorocatechol 2,3-dioxygenase (cbzE) was cloned and sequenced. The cbzE gene appeared to be plasmid localized and was found in a region that also harbors genes encoding a transposase, a ferredoxin that was homologous to XylT, an open reading frame with similarity to a protein of a meta-cleavage pathway with unknown function, and a 2-hydroxymuconic semialdehyde dehydrogenase. CbzE was most similar to catechol 2,3-dioxygenases of the 2.C subfamily of type 1 extradiol dioxygenases (L. D. Eltis and J. T. Bolin, J. Bacteriol. 178:5930-5937, 1996). The substrate range and turnover capacity with 3-chlorocatechol were determined for CbzE and four related catechol 2,3-dioxygenases. The results showed that CbzE was the only enzyme that could productively convert 3-chlorocatechol. Besides, CbzE was less susceptible to inactivation by methylated catechols. Hybrid enzymes that were made of CzbE and the catechol 2, 3-dioxygenase of P. putida UCC2 (TdnC) showed that the resistance of CbzE to suicide inactivation and its substrate specificity were mainly determined by the C-terminal region of the protein.     

cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes

A cDNA fingerprinting strategy was developed to identify genes based on their differential expression pattern during osteoblast development. Preliminary biological and molecular staging of cDNA pools prepared by global amplification PCR allowed discrim-inating choices to be made in selection of expressed sequence tags (ESTs) to be isolated. Sequencing of selected ESTs confirmed that both known and novel genes can be isolated from any developmental stage of interest, e.g. from primitive progenitors, intermediate precursors or mature osteoblasts. EST expression provides insight into possible interrelated physiological functions and putative interacting molecules during differentiation. This method offers a functional genomics approach to isolate differentiation stage-specific genes in samples as small as a single cell.     

嵌入式Linux在新型仪器交叉站中的应用

本文介绍了一种基于嵌入武Linux的交叉站设计方案,分别从交叉站的硬件平台、嵌入式Linux操作系统以及系统应用软件设计流程等方面进行阐述;并利用Intel XScale系列的IXP460微处理器实现了嵌入式Linux的交叉站设计.通过测试和实验,达到了预期的设计要求,显示了嵌入式系统具有针对性和实时性的设计,提高了资源利用率,成本低,性能高,并具备应用软件在系统可升级的功能.     

Continuum Bed Model for Estuarine Sediments Based on Nonlinear Consolidation Theory

The goal of this note is to examine a continuum theory that describes the evolution of sediment beds when subjected to time-dependent shearing forces resulting from surface water movement. The bed was conceptualized as a medium with continuously varying properties such as shear strength and void ratio. The nonlinear equation describing finite strain consolidation, and the complicated nature of the shearing forces acting on top of the bed, preclude the possibility of analytical solutions. Ramifications of linearizing the governing flow equations were explicitly evaluated for applications in bed modeling. Numerical solutions were obtained for the linear and nonlinear models under transient boundary conditions. Model results indicated that the linear model typically predicts lower void ratios, and consequently underestimates the amounts of sediment eroded from the bed as compared to the nonlinear model.     

Progressive bounds on merit factor

Two usual criteria of goodness of pulse compression sequences are the discrimination and the merit factor. Using the notion of equivalence of l/sub p/ norms, they are related by an inequality. Using bounds on the aperiodic autocorrelation, the inequality is strengthened further to obtain a bound on the merit factor in terms of the length of the sequence and the Barker progression number.<>     

江西于都天然琉璃釉的研发

鉴于江西于都发现天然黄绿色琉璃釉资源，从资源概况、琉璃釉工艺特征和应用效果等方面对其进行了初步评价，并对其成釉机理做了简单探讨。     

An improved iterative finite element solution for pin joints

Accurate, reliable and economical methods of determining stress distributions are important for fastener joints. In the past the contact stress problems in these mechanically fastened joints using interference or push or clearance fit pins were solved using both inverse and iterative techniques. Inverse techniques were found to be most efficient, but at times inadequate in the presence of asymmetries. Iterative techniques based on the finite element method of analysis have wider applications, but they have the major drawbacks of being expensive and time-consuming. In this paper an improved finite element technique for iteration is presented to overcome these drawbacks. The improved iterative technique employs a frontal solver for elimination of variables not requiring iteration, by creation of a dummy element. This automatically results in a large reduction in computer time and in the size of the problem to be handled during iteration. Numerical results are compared with those available in the literature. The method is used to study an eccentrically located pin in a quasi-isotropic laminated plate under uniform tension.     

Opioid receptor-coupled G-proteins in rat locus coeruleus membranes: decrease in activity after chronic morphine treatment

The nucleus locus coeruleus is involved in the expression of opiate physical dependence and withdrawal, and has been characterized extensively with regard to chronic morphine-induced alterations in biochemical and electrophysiological responses. In the present study the effects of chronic morphine treatment on opioid receptor-coupled G-protein activity was investigated in membranes from rat locus coeruleus. Opioid agonists stimulated low Km GTPase activity with pharmacology consistent with mu receptors. Chronic morphine treatment resulted in decreases in both basal and opioid-stimulated low Km GTPase activity, with no change in the percent stimulation by agonist. The decrease in low Km GTPase activity appeared to be due to a decrease in the Vmax of the enzyme, with no change in the Km for GTP hydrolysis. These results were confirmed by assays of basal and opioid receptor-stimulated [35S]GTP gamma S binding in the presence of excess GDP. Thus, chronic morphine treatment apparently decreased inhibitory G-protein activity in the locus coeruleus without producing any detectable desensitization. These results suggest a potential adaptation at the receptor/transducer level which may contribute to other biochemical changes produced in the locus coeruleus by chronic morphine treatment.