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In experimental science measurements are typically repeated only a few times, yielding a sample size n of the order of 3 to 8. One then wants to summarize the measurements by a central value and measure their variability, i.e. estimate location and scale. These estimates should preferably be robust against outliers, as reflected by their small-sample breakdown value. The estimator's stylized empirical influence function should be smooth, monotone increasing for location, and decreasing–increasing for scale. It turns out that location can be estimated robustly for n3, whereas for scale n4 is needed. Several well-known robust estimators are studied for small n, yielding some surprising results. For instance, the Hodges–Lehmann estimator equals the average when n=4. Also location M-estimators with auxiliary scale are studied, addressing issues like the difference between one-step and fully iterated M-estimators. Simultaneous M-estimators of location and scale (‘Huber's Proposal 2’) are considered as well, and it turns out that their lack of robustness is already noticeable for such small samples. Recommendations are given as to which estimators to use.  相似文献   
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Euler diagrams are a popular technique to visualize set-typed data. However, creating diagrams using simple shapes remains a challenging problem for many complex, real-life datasets. To solve this, we propose RectEuler: a flexible, fully-automatic method using rectangles to create Euler-like diagrams. We use an efficient mixed-integer optimization scheme to place set labels and element representatives (e.g., text or images) in conjunction with rectangles describing the sets. By defining appropriate constraints, we adhere to well-formedness properties and aesthetic considerations. If a dataset cannot be created within a reasonable time or at all, we iteratively split the diagram into multiple components until a drawable solution is found. Redundant encoding of the set membership using dots and set lines improves the readability of the diagram. Our web tool lets users see how the layout changes throughout the optimization process and provides interactive explanations. For evaluation, we perform quantitative and qualitative analysis across different datasets and compare our method to state-of-the-art Euler diagram generation methods.  相似文献   
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The human scalp is characterized by a moderately diverse microbial community, comprising prokaryotic (bacteria) and eukaryotic (fungi) members. Although the details are far from being fully understood, the human scalp microbiota is implicated in several scalp disorders, in particular dandruff formation. Hence, the protection of an intact and diverse scalp microbiota can be regarded as a quality criterion for hair and scalp care formulations. In this study, we investigated the influence of two commercially available, non-antimicrobial shampoo formulations on the structure of the scalp microbiota.  相似文献   
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The antimicrobial equipment of materials is of great importance in medicine but also in daily life. A challenge is the antimicrobial modification of hydrophobic surfaces without increasing their low surface energy. This is particularly important for silicone‐based materials. Because most antimicrobial surface modifications render the materials more hydrophilic, methods are needed to achieve antimicrobial activity without changing the high water‐contact‐angle. This is achieved in the present work, where SiO2 nanoparticles are prepared and functionalized with 3‐(trimethoxysilyl)‐propyldimethyloctadecyl ammonium chloride (QAS) in a one‐pot synthesis. The modified nanoparticles are applied onto a silicone surface from suspension with no need of elaborate pretreatment. The resulting surface exhibits a Lotus‐Effect combined with contact‐active antimicrobial properties. The particle surfaces show self‐organizing micro‐ and nanostructures that afford a water‐contact angle of 144° and a hysteresis below 10°. The particles are self‐adhering on the silicone after solvent evaporation and resistant against immersion into and washing with water for at least 5 d. Thereby, the adhesion of the bacterial strain Staphylococcus aureus to these surfaces is reduced and the remaining bacterial cells are killed within 16 h. This is the first example of a Lotus‐Effect surface with intrinsic contact‐active antimicrobial properties.  相似文献   
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Inteins carry out protein-splicing reactions, which are used in protein chemistry, protein engineering and biotechnological applications. Rearrangement of the order of the domains in split-inteins results in head-to-tail cyclisation of the target sequence, which can be used for genetic encoding and expression of libraries of cyclic peptides (CPs). The efficiency of the splicing reaction depends on the target sequence. Here we used mass spectrometry to assess in vivo cyclic peptide formation from different hexameric target sequences by the DnaE split-inteins from Synechocystis sp. and Nostoc punctiforme, revealing a strong impact of the target sequence and of the intein on the intracellular peptide concentration. Furthermore, we determined the crystal structures of their pre-splicing complexes, which allowed us to identify F-block Asp17 as crucial for the DnaE-mediated splicing reaction.  相似文献   
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