On the Relationship of Sample Size and Moments of Estimators in Simple Linear Regression

Methods are developed for determining minimum sample size in simple linear regression (when residuals are nonnormal) that permit use of the classical normality-based analyses. The methods are based on behavior of standardized third and fourth moments of regression estimators. The case of symmetric independent variable values with one observation at each is considered. All other regression assumptions are assumed to be true.     

Expression profile of T cell associated molecules in the interfacial tissue of aseptically loosened prosthetic joints

The involvement of T cells in the progression of inflammation in response to wear debris at the interface of aseptically loosened joints is currently undefined. This cell type has repeatedly been demonstrated to be a common component of the cellular membrane, the interface, which forms between the bone and implant of total joint replacements (TJRs) [1, 2]. Three further insights into the role of this cell type in the interface were investigated here. Immunostaining demonstrated CD4 expression in 80% of the 15 cases tested while CD8 expression was present in 60% of the cases. Polymerase chain reaction (PCR) detected IFN- mRNA expression in 75% of eight cases tested; in contrast IL-10 mRNA was only demonstrated in 50% of these same cases. Proteins extracted from another eight cases of revision tissue were analyzed using Western blotting for IL-17, fractalkine (Fkn) and CD40. IL-17 and Fkn were a consistent feature of all cases tested (8/8), while CD40 was undetectable in one case (7/8). These results show that T cells present in the interface are more commonly of the helper T cell phenotype, although cytotoxic T cells are also present. Helper T cells (Th) are responsible for the polarization of the immune response through their production of key mediators. The PCR results obtained in this study suggest that a Th1 response characterized by the production of IFN- predominates over the Th2, IL-10 mediated response. Furthermore the demonstration of the expression of IL-17, Fkn and CD40, all of which are Th1 associated molecules, supports this conclusion.     

Multi-Modal Interaction of Human and Home Robot in the Context of Room Map Generation

In robotics, the idea of human and robot interaction is receiving a lot of attention lately. In this paper, we describe a multi-modal system for generating a map of the environment through interaction of a human and home robot. This system enables people to teach a newcomer robot different attributes of objects and places in the room through speech commands and hand gestures. The robot learns about size, position, and topological relations between objects, and produces a map of the room based on knowledge learned through communication with the human. The developed system consists of several sections including: natural language processing, posture recognition, object localization and map generation. This system combines multiple sources of information and model matching to detect and track a human hand so that the user can point toward an object of interest and guide the robot to either go near it or to locate that object's position in the room. The positions of objects in the room are located by monocular camera vision and depth from focus method.     

Influence of the valve lubricant on the aerodynamic particle size of a metered dose inhaler

Presented in this work are the results of a study designed to investigate the impact of valve lubricant (i.e., silicone oil) on the aerodynamic particle size distribution (PSD) of a steroid suspension metered dose inhaler (MDI) containing propellant HFA-227. The objective of this study was to explore whether the valve lubricant, which is often used in MDI products to prevent valve sticking, can enter an MDI product and potentially impact the aerosol spray dynamics. The results of this work have shown that samples containing valves with high silicone levels produced a larger aerodynamic particle size (by cascade impaction) than samples with low-silicone or silicone-free valves. It is postulated that the presence of silicone in the product may increase the propensity for drug aggregation, thereby leading to an increase in the aerodynamic particle size of the emitted aerosol. These findings stress the importance of evaluating the effects of valve lubricant on the aerodynamic PSD in the early formulation development stage of an MDI.     

Entry port selection for detecting particle size differences in metered dose inhaler formulations using cascade impaction

Different sized glass entry ports were evaluated for their drug collection efficiency during aerodynamic particle sizing of metered dose inhalers (MDIs) using cascade impaction. A comparison was made between collection efficiency in the entry port, impactor plates, and filter using the 1 L, 2 L, and 20 L glass entry ports and the USP and twin impinger entry ports. Entry port losses were dependent on the size of entry port selected, with 1-2 L ports showing optimal recovery on impactor plates, compared to the USP entry port. The 1 L entry port was further compared with the USP entry port in its ability to discriminate between subtle changes in particle size distribution (PSD) in an investigational hydrofluoroalkane (HFA)-based MDI formulation. Deliberately induced differences during product manufacture were easily detected using the 1 L entry port with the Andersen cascade impactor. The USP port was unable to distinguish among products with small particle size differences. An alternative entry port such as the 1 L glass entry port used in this study may provide better means of characterizing the PSD during formulation development and stability testing of MDIs.     

Influence of the size of micronized active pharmaceutical ingredient on the aerodynamic particle size and stability of a metered dose inhaler

Pharmaceutical inhalers are often used to treat pulmonary diseases. Only active pharmaceutical ingredient (API) particles from these inhalers that are less than approximately 5 microm are likely to reach the lung and be efficacious. This study was designed to investigate the impact of micronized API particle size on the aerodynamic particle size distribution (PSD) profile and the particle size stability of a suspension metered dose inhaler (MDI) containing propellant HFA-227 (1,1,1,2,3,3,3 heptafluoropropane) and a corticosteroid. The median API particle size ranged from 1.1 microm to 1.8 microm (97% to 70% of particles <3 microm, respectively). This study showed that increasing the particle size of the API used to manufacture a suspension MDI product increased the aerodynamic PSD of the MDI product. Furthermore, upon storage of the MDI product under temperature cycling conditions, samples containing larger-size API particles were less stable with respect to their aerodynamic PSD than those with smaller-size API particles. It was found that size-dependent particle growth and/or aggregation of the suspended API may be occurring as a result of temperature cycling. In conclusion, this study has shown that the particle size of the raw API impacts the properties and stability of the emitted aerosol spray. Based on the findings from this study, it is recommended that the API particle size be carefully controlled in order to meet specifications set for the finished MDI product.     

DFB Lasers With Deeply Etched Vertical Grating Based on InAs–InP Quantum-Dash Structures

Distributed feedback lasers with first-order vertical grating based on AlInGaAs-InAs-InP quantum-dash lasers were fabricated by electron beam lithography and Cl₂-Ar reactive ion etching with an electron cyclotron resonance source. Low threshold currents and single-mode operation with sidemode suppression ratios of 48 dB and a direct modulation bandwidth of 5.5 GHz were demonstrated     

A New Approach to Parallelization of Serial Nested Loops Using Genetic Algorithms

Loop parallelization is an important issue in the acceleration of the execution of scientific programs. To exploit parallelism in loops a system of equations representing the dependencies between the loop iterations and a system of non-equations indicating the loop boundary conditions has to be solved. This is a NP-Complete problem. Our major contribution in this paper has been to apply genetic algorithm to solve system of equation and non-equation resulted from loop dependency analysis techniques to find two dependent loop iterations. We use distance vector to find the rest of dependencies.     

An improved wavelet-based speech enhancement by using speech signal features

This paper presents a new speech enhancement system that works in wavelet domain. The core of system is an improved WaveShrink module. First, different parameters of WaveShrink are studied; then, based on the features of speech signal, an improved wavelet-based speech enhancement system is proposed. The system uses a novel thresholding algorithm, and introduces a new method for threshold selection. Moreover, the efficiency of system has been increased by selecting more suitable parameters for voiced, unvoiced and silence regions, separately. The proposed system has been evaluated on different sentences under various noise conditions. The results show a plausible improvement in performance of system, in comparison with similar approaches.     

Some New Organometallic Polyamides based on Ferrocene

Three new polyamides containing ferrocene units in the main chain were synthesized via low temperature polycondensation route, reacting 1,1′-ferrocenedicarboxylic acid chloride with three different types of aromatic diamines. The products were characterized by their solubilities, elemental analysis, FTIR spectral analysis, differential scanning calorimetry, thermogravimetry and viscosity measurements. The glass transition temperatures of the polymers were found by DSC curves and the activation energies of pyrolysis were estimated from TG curves applying Horowitz and Metzger method. Among these, polyamide P-1 (prepared from 4-(4-aminophenyloxy)phenyl-4-aminobenzamide) was found soluble in some of the organic solvents at room temperature but has poor thermal stability. Polyamide P-2 (prepared from 1,2-di(para-aminophenyloxy)ethylene) was soluble on heating and is thermally stable. However, all of these were also miscible with concentrated H₂SO₄ forming red coloured solutions.

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Graphical Abstract Ferrocenedicarbonyl chloride was reacted with three different types of aromatic diamines via solution polycondensation to get three new polyamides. Two of which were soluble in some organic solvents. The resulting polymers were characterized by their solubilities, viscosity measurements, elemental analysis, FTIR spectroscopy, differential scanning calorimetric and thermogravimetric studies.