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71.
    
Wheat blast, caused by the fungus Magnaporthe oryzae Triticum (MoT) pathotype, is a devastating disease persistent in South America and Bangladesh. Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced, disease control by fungicide application solely based on the detection of visual symptoms is ineffective. To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control, we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments, MoT-6098 and MoT-6099, that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae (MoO) pathotype. Using polymerase chain reaction (PCR), we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh. To test the efficiency of the two markers, we first established a loop-mediated isothermal amplification (LAMP) method to detect MoT at isothermal conditions, without the use of a PCR machine. Following this, we used the Cas12a protein and guide RNAs (gRNAs) to target the MoT-6098 and MoT-6099 sequences. The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease (ssDNase) activity. We then combined target-dependent Cas12a ssDNase activation with recombinase polymerase amplification (RPA) and nucleic acid lateral flow immunoassay (NALFIA) to develop a method that accurately, sensitively, and cost-effectively detects MoT-specific DNA sequences in infected wheat plants. This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.  相似文献   
72.
    
In this paper, a recovery model is developed for managing sudden supply delays that affect retailers’ economic order quantity model. For this, a mathematical model is developed that considers fuzzy demand and safety stock, and generates a recovery plan for a finite future period immediately after a sudden supply delay. An efficient heuristic solution is developed that generates the recovery plan after a sudden supply delay. An experiment with scenario-based analysis is conducted to test our heuristic and to analyse the results. To assess the quality and consistency of solutions, the performance of the proposed heuristic is compared with the performance of the generalised reduced gradient method, which is widely applied in constrained mathematical programming. A simulation model is also designed to bring the recovery model closer to real-world processes. Several numerical examples are presented and a sensitivity analysis is performed to demonstrate the effects of various parameters on the performance of the heuristic method. The results show that safety stock plays an important role in recovery from sudden supply delays, and there is a trade-off between backorder and lost sales costs in the recovery plan. With the help of the proposed model, supply chain decision-makers can make accurate and prompt decision regarding recovery plans in case of sudden supply delay.  相似文献   
73.
    
A robust consensus controller is proposed for heterogeneous higher‐order nonlinear multi‐agent systems, when the agent dynamics are involved with mismatched uncertainties. A distributed consensus protocol based on a time‐varying nonhomogeneous finite‐time disturbance observer and sliding mode control is designed to realize the network consensus of higher‐order multi‐agent systems. The time‐varying finite‐time disturbance observer overcomes the problem of peaking value near the initial time caused by the constant gain one and is designed to estimate the uncertainties and to mitigate the effect of mismatched uncertainties during the sliding mode. To eliminate the chattering phenomenon and ensure finite‐time convergence to the sliding surface, the control law is designed by using the super twisting algorithm. Finally numerical simulations are given to illustrate the validity of the proposed method. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   
74.
    
The efficacy of woven jute-polyester (JP) composite tubes as structural energy-absorbing countermeasures has been explored in the current study. In this connection, the behaviors of 3-ply and 4-ply JP composite tubes of square and double-hat shaped sections subjected to axial quasi-static and impact loads are considered, and compared with 4-ply glass-polyester (GP) tubes of similar geometric configurations. Initially, basic mechanical characterization of JP laminates is carried out using a Universal Testing Machine, which is followed up with axial quasi-static and drop-weight impact testing of JP tubes, along with similar tests carried out on GP tubes. A thorough comparison is made between the performances including failure patterns of JP and GP components under axial loading using metrics such as peak load, mean crush load, absorbed energy, and specific energy absorption (SEA). As many structural applications involve dynamic bending loads, a comparative study is carried out between double-hat JP and GP components subjected to transverse impact loading. Based on the competitive values of mean load and SEA yielded by jute-composite tubes in the present study for axial impact loading, and high mean load and moderate energy absorption till failure recorded by similar components under three-point impact bending, it can be concluded that JP tubes can be capable of supporting impact loads under both axial and bending modes.  相似文献   
75.
    
This work aims to develop smart stimuli responsive composites utilizing hydrogenated nitrile rubber (HNBR), fly ash (FA), and slag (SG) with white cement (WC). HNBR has been chosen by capitalizing its unique attributes of high temperature performance and high oil resistance as the matrix and coining the synergistic setting characteristics of FA and SG as industrial wastes with WC. The composites displayed synergism in mechanical and thermal properties. The calcium-rich phases of FA and SG, entice the postpolymerization modification of the rubber matrix in conjunction with cement serving as reactive fillers. The changes of modulus (~50 MPa), mass (~7%), volume (~8%), and hardness were observed upon repeated wet-dry cycles. These are attributed to the ionic interactions between the modified polymer matrix and cementitious phases as a result of postpolymerization conversion. These smart composite materials could be used for sealing and microfluidic valves for the downhole applications in oil field industries.  相似文献   
76.
    
V2O5 electrochromic (EC) coatings are attractive for various photonic applications owing to their multiple oxidation states discernable by distinct colors. The material also shows massive potential for electrochemical energy storage because of their layered structure and high theoretical capacitance. Here, the solution processing of the electrodes composed of an ultra‐thin layer of MnO2‐encrusted V2O5 (V2O5/MnO2) nanowire mats on fluorinated tin oxide substrates is reported that offer much enhanced electrochemical stability along with a superior energy storage performance compared to a bare V2O5 electrode. The areal capacitance of the V2O5 shows 25.7% enhancement (10.90 to 13.70 mF cm−2 at 5.0 mV cm−2) due to MnO2 encrustation. The fabricated EC devices show three distinct colors, i.e., orange (+3.0 V), green (−2.5 V), and blue (−3.0 V). Capacitance retention of bare V2O5 at 1000 cycles is 71% that increases to 93.6% after MnO2 coating due to the enhanced electrochemical stability.  相似文献   
77.
Higher production, better safety standard, and potential for automation are some of the benefits of Iongwall mining. Today, Iongwall face advances at a faster rate exposing many diversifted rock layers in a short period of time. It is now a serious challenge to cope with ground control problems such as roof falls, face and floor failure, and excessive shield loading as fast as possible to minimize production and monetary losses. In Illinois Coal Mines, the existence of weak floor strata blow the coal seam may pose additional problems related to floor heaving, shield base punching, and associated roof and face falls. In this study, the effects of weak floor on Iongwall ground control are analyzed using two-dimensional finite element models. A two-leg 635.6 ton (700-short-ton) yielding capacity shield is included in the models to evaluate the effects of different thickness and material properties of the weak floor. The study indicates that the thickness and material properties of weak floor have significant effects on shield loading, the distribution and intensity of front abutment stress, failure zones in the surrounding strata, roof-to-floor convergence, and floor punching by the shieldbase.  相似文献   
78.
Network-Wide Optimal Scheduling of Transit Systems Using Genetic Algorithms   总被引:1,自引:0,他引:1  
The primary objective of any transit system is to provide a better level of service to its passengers. One of the good measures of level of service is the waiting time of passengers during their journey. The waiting time consists of an initial waiting time (the time a passenger waits to board a vehicle at his or her point of origin) and a transfer time (the time a passenger waits at a transfer station while transferring from one vehicle to another). An efficient schedule minimizes the overall transfer time (TT) of passengers transferring between different routes as well as the initial waiting time (IWT) of the passengers waiting to board the vehicle at their point of origin. This paper uses genetic algorithm (GA)—a search and optimization procedure—to find optimal/near-optimal schedules of vehicles in a transit network. The main advantage of using GA is that the transit network scheduling problem can be reformulated in a manner that is computationally more efficient than the original problem. Further, the coding aspect of GA inherently takes care of most of the constraints associated with the scheduling problem. Results from a number of test problems show that GAs are able to find optimal/near-optimal schedules with minimal computational resources.  相似文献   
79.
The experimental results of an air-core pulse transformer are presented, which is very compact (<10 Kg in weight) and is primed by a capacitor bank that is fabricated in such a way that the capacitor bank with its switch takes the shape of single-turn rectangular shaped primary of the transformer. A high voltage capacitor assembly (pulse-forming-line capacitor, PFL) of 5.1 nF is connected with the secondary of transformer. The transformer output voltage is 160 kV in its second peak appearing in less than 2 μS from the beginning of the capacitor discharge. The primary capacitor bank can be charged up to a maximum of 18 kV, with the voltage delivery of 360 kV in similar capacitive loads.  相似文献   
80.
The implications of the ageing of the Australian population can be experienced at a micro level in any number of municipalities around Victoria and Australia. The cyclical nature of household formation is such that disproportionate representations of specific age groups can be evident in particular areas at particular times.  相似文献   
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