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61.
Full analysis of triglycerides of natural fats and oils has been investigated by the combination of argentation high performance
liquid chromatography (HPLC) with nonaqueous reversed phase (NARP) HPLC. An infrared detector was used in argentation HPLC,
because it indicated molar responsibility to all triglycerides. After peak trapping with argentation HPLC, each triglyceride
fraction was analyzed with NARP chromatography using the glyceride-selective post-column reactor detector. The results of
the analyses of triglycerides of palm oil and cocoa butter by the proposed method agreed well with those reported earlier. 相似文献
62.
The potential protection of Picea glehnii seedlings from damping-off by seed-epiphytic Penicillium species was investigated. We studied the chemical response of seed-epiphytic Penicillium species (Pen. cyaneum, Pen. damascenum, and Pen. implicatum) to Pythium vexans, a damping-off fungus, in vitro. Penicillium species were cultured singly or cocultured with Pyt. vexans for 14 or 18 d, and mycelial growth, pH of culture filtrate, antifungal activity of the culture filtrate against Pyt. vexans, and the amount of antifungal compound produced by each Penicillium species, were examined. The filtrate of both the single culture of Penicillium and the coculture of Penicillium and Pyt. vexans showed antifungal activity against Pyt. vexans. In a coculture with Pyt. vexans, Pen. cyaneum produced an antifungal compound (patulin) as in the single culture. Pen. damascenum cocultured with Pyt. vexans produced an antifungal compound (citrinin), as it did in the single culture and in larger amounts on day 10. Pen. implicatum produced two antifungal compounds, frequentin and palitantin, and the ratio of frequentin (with higher antifungal activity than palitantin) to palitantin was higher in the coculture with Pyt. vexans than in the single culture. Our results indicate that these Penicillium species have the ability to produce antifungal compounds and to keep antifungal activity under competitive condition with Pyt. vexans. The chemical response of these Penicillium species to Pyt. vexans may contribute to protect P. glehnii seedlings from damage by Pyt. vexans. 相似文献
63.
Thin SiO2 layers were deposited by atomic layer deposition (ALD) using either Bis-dimethylamino-silane (BDMAS: SiH2(N(CH3)2)2) or Tris-dimethylamino-silane (TDMAS: SiH(N(CH3)2)3) precursors. The purpose of this study is to evaluate these precursors for their suitability for ALD of hafnium (Hf)-silicate gate dielectrics. The advantages of these precursors are that the melting points and vapor pressures are moderate. The thickness of SiO2 deposited using ALD process is controlled by the number of growth cycles and the growth rate was different for each precursor, that for BDMAS being 1.5 times that for TDMAS at the same reactor pressure. The carbon impurity in the SiO2 film deposited using BDMAS was about half an order of magnitude less than that using for TDMAS. Furthermore, the carbon impurity was reduced to about the detection limit of secondary ion mass spectrometry after high temperature annealing at 1000 °C during 5 s. 相似文献
64.
Theory of Three-Dimensional Discontinuous Deformation Analysis and Its Application to a Slope Toppling at Amatoribashi, Japan 总被引:1,自引:0,他引:1
Jian-Hong Wu Yuzo Ohnishi Gen-Hua Shi Satoshi Nishiyama 《Canadian Metallurgical Quarterly》2005,5(3):179-195
A three-dimensional (3D) rock slope toppling occurred in a discontinuous rock mass. To simulate the failure process and study the mechanism of this rock failure with contact and large displacement in 3D, a new discrete numerical method has been developed called the 3D discontinuous deformation analysis (DDA). This article first introduces the basic principles and then derives the formulas in detail. Finally, the slope failure simulation is applied as an example to investigate the applicability of this new method to rock slope failure research. The simulation results indicate the advantages of using this new method to study the mechanism of a rock slope failure with 3D behavior. 相似文献
65.
Satoshi Izumi Chan Wee Ping Shinsuke Sakai Atsushi Suzuki 《Engineering Fracture Mechanics》2005,72(17):2672-2685
Specimens and fracture test methods for strength analysis of MEMS micromirrors were proposed. Bending and combined loading tests were performed, and torsion strength was estimated from those results. Two-parameter Weibull distribution was used to evaluate the fracture stresses estimated from the FEM model. The resulting scale and shape parameters were 787 MPa and 7.77 for the bending test and 517 MPa and 5.28 for the combined loading test. There was a difference in strength between the results of the bending and combined loading tests. From the load factor analysis, it was found that both geometry and stress distribution have to be considered to estimate the strength of MEMS since flaws are non-uniformly distributed. It was also found that torsional strength can be estimated on the safe side using the results of the combined loading test. 相似文献
66.
67.
Scott L. Huang Matthew T. Bray Satoshi Akagawa Masami Fukuda 《Canadian Metallurgical Quarterly》2004,18(1):2-34
A large scale 105-m-long, 0.9-m-diam chilled pipeline experiment was conducted to assess the response of a production pipeline that transits between unfrozen (seasonally frozen) and permafrost soils in discontinuous permafrost regions. Thermal and heave characteristics of the experiment for a three-year monitoring period are reported. In response to the chilled pipeline, the progressive cooling patterns within the permafrost and unfrozen soils were different, resulting in the development of a thermal boundary at the transition zone between the two thermally different soils. The absolute maximum movement of pipeline was 0.197 m near the thermal boundary, resulting in a differential heave of 0.148 m. Three distinct heave phases were identified within the unfrozen soil. Phase 1 of the first 200 days related to penetration and heave above the groundwater table and was characterized by heave rates between 0.211 and 0.237 mm/day. Phase 2 resulted in increased ice segregation due to interaction of the freez-ing front with the groundwater table. This phase lasted from approximately day200 to day 500 and corresponded to a heave rate from 0.206 to 0.313 mm/day. Phase 3 was characterized by a further decrease in the heave rate to about 0.081 mm/day and lasted from approximately day 500 to day 1,056. 相似文献
68.
In this paper, we present a numerical algorithm to the cross‐coupled algebraic Riccati equations(CARE) related to H2/H∞ control problems for singularly perturbed systems (SPS) by means of Newton's method. The resulting algorithm can be widely used to solve Nash game problems and robust control problems because the CARE is solvable even if the quadratic term has an indefinite sign. We prove that the resulting iterative algorithm has the property of the quadratic convergence. Using the solution of the CARE, we construct the high‐order approximate H2/H∞ controller. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
69.
Satoshi Suzuki Yaodong Pan Katsuhisa Furuta Shoshiro Hatakeyama 《Asian journal of control》2004,6(3):307-316
In general, a Variable Structure (VS) system is designed with a sliding mode. Recently a sliding sector, designed by an algebraic Riccati equation, has been proposed to replace the sliding mode for chattering‐free VS controllers. In this paper we extend the design algorithm for the sliding sector to a time‐varying sliding sector. The time‐varying sliding sector is defined by functions dependent on both state and time, hence time‐varying uncertainty can be considered. The VS controller is designed to stabilize an uncertain system, quadratically. The design procedure for real systems is introduced via an implementation to the control of “Furuta pendulum”. To enhance the stability it is necessary to compensate the time‐varying nonlinear static friction of the actuator adequately, hence this problem is a good example to demonstrate the performance of the proposed VS control method. In the experiment, it will be shown that the VS control with the time‐varying sliding sector is superior to an orthodox chattering‐free VS control. 相似文献
70.
Yanyan Hou Satoshi Arai Yoshiaki Takei Atsushi Murata Shinji Takeoka 《Science and Technology of Advanced Materials》2016,17(1):293-299
Ca2+ distribution is spatially and temporally non-uniform inside cells due to cellular compartmentalization. However, Ca2+ sensing with small organic dyes, such as fura-2 and fluo-4, has been practically applied at a single cell level where the averaged signal from freely diffusing dye molecules is acquired. In this study, we aimed to target azide-functionalized fura-2 (N3-fura-2) to a specific site of subcellular compartments to realize focal Ca2+ sensing. Using scAVD (single-chain avidin)–biotin interaction and a copper-free click reaction system, we linked N3-fura-2 to specifically-targeted scAVD protein fused with a red fluorescent protein mCherry, so that Ca2+ sensors conjugated with four N3-fura-2 dyes with dibenzocyclooctyne (DBCO)-PEG4-biotin as a linker were generated at subcellular compartments in living cells. In cytoplasm, N3-fura-2 showed a prolonged retention period after binding to scAVD. Furthermore, the reacted N3-fura-2 was retained inside cells even after free dyes were washed out by methanol fixation. When scAVD was overexpressed on endoplasmic reticulum (ER) membranes, N3-fura-2 was accumulated on ER membranes. Upon histamine stimulation, which increases cytosolic Ca2+ concentration, ER-localized N3-fura-2 successfully sensed the Ca2+ level changes at the cytosolic side of ER membrane. Our study demonstrated specific targeting of N3-fura-2 to subcellular compartments and the ability of sensing focal Ca2+ level changes with the specifically targeted Ca2+ sensors. 相似文献