Network Architectures and Performance

The capability of networks to handle various forms of communications traffic and to interconnect is based on several network characteristics, most important, capacity and utilization. A review of available network architectures and their abilities to handle increasing user demand while maintaining reliability in a bandwidth-intensive environment helps sort out today's maze of networking options.     

A comprehensive panel of near-full-length clones and reference sequences for non-subtype B isolates of human immunodeficiency virus type 1

Non-subtype B viruses cause the vast majority of new human immunodeficiency virus type 1 (HIV-1) infections worldwide and are thus the major focus of international vaccine efforts. Although their geographic dissemination is carefully monitored, their immunogenic and biological properties remain largely unknown, in part because well-characterized virological reference reagents are lacking. In particular, full-length clones and sequences are rare, since subtype classification is frequently based on small PCR-derived viral fragments. There are only five proviral clones available for viruses other than subtype B, and these represent only 3 of the 10 proposed (group M) sequence subtypes. This lack of reference sequences also confounds the identification and analysis of mosaic (recombinant) genomes, which appear to be arising with increasing frequency in areas where multiple sequence subtypes cocirculate. To generate a more representative panel of non-subtype B reference reagents, we have cloned (by long PCR or lambda phage techniques) and sequenced 10 near-full-length HIV-1 genomes (lacking less than 80 bp of long terminal repeat sequences) from primary isolates collected at major epicenters of the global AIDS pandemic. Detailed phylogenetic analyses identified six that represented nonrecombinant members of HIV-1 subtypes A (92UG037.1), C (92BR025. 8), D (84ZR085.1 and 94UG114.1), F (93BR020.1), and H (90CF056.1), the last two comprising the first full-length examples of these subtypes. Four others were found to be complex mosaics of subtypes A and C (92RW009.6), A and G (92NG083.2 and 92NG003.1), and B and F (93BR029.4), again emphasizing the impact of intersubtype recombination on global HIV-1 diversification. Although a number of clones had frameshift mutations or translational stop codons in major open reading frames, all the genomes contained a complete set of genes and three had intact genomic organizations without inactivating mutations. Reconstruction of one of these (94UG114.1) yielded replication-competent virus that grew to high titers in normal donor peripheral blood mononuclear cell cultures. This panel of non-subtype B reference genomes should prove valuable for structure-function studies of genetically diverse viral gene products, the generation of subtype-specific immunological reagents, and the production of DNA- and protein-based subunit vaccines directed against a broader spectrum of viruses.     

Aromatase-immunoreactive neurons in the adult female chicken brain detected using a specific antibody

Estrogen formation in the brain catalysed by the cytochrome P450arom is required for the control of estrogen-dependent neural mechanisms regulating reproductive behaviour. A polyclonal antibody was raised against a 15-amino acid fragment of the chicken ovarian P450arom protein, to localise aromatase-immunoreactive (AR-IR) cells in the adult female chicken brain. Specificity of antibody reaction was established by Western blot and by inhibition of aromatase activity in homogenates of chicken ovarian follicles determined by a radiometric assay. The AR-IR material in the brain was localised in the perikarya and some of their adjacent cytoplasmatic processes. Intense immunoreactivity was observed in the preoptic region as well as in other hypothalamic nuclei. AR-IR cells were also found in extrahypothalamic areas; in particular, in the area entorhinalis and hippocampus. These results confirm histologically that aromatization of testosterone in the adult female chicken brain occurs in preoptic nuclei closely associated with the regulation of reproductive behaviour. The mapping of AR-IR cells in the female chicken brain now allows study of its regulation under different physiological and environmental conditions, and its relation to classic target areas expressing estrogen receptors.     

Knowledge, interest, and narrative writing.

One-hundred-six 9th graders and 203 undergraduates wrote a story about baseball for 25 min and then completed a 39-item multiple-choice test of baseball topic knowledge. Students also answered 6 questions about their individual interest in baseball. Confirmatory factor analysis suggested that knowledge and interest tests measured different constructs. Hierarchical regression analyses revealed Grade?×?Interest and Gender?×?Interest interactions on thematic maturity. Differences favoring undergraduates at low levels of interest disappeared at higher levels of interest, and differences favoring male students at low levels of interest disappeared at higher levels of interest. Topic knowledge predicted thematic maturity and was a better predictor of the interestingness of students' written texts than was individual interest. Implications for the assignment of student writing topics are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Quantitative and densitometric considerations in bas-relief electron micrographs

Two methods for bas-relief electron micrographs are described, one to produce transparencies for projection and one to produce prints for publication. The basis of these methods is a controlled misregistration of a positive image and a negative image, both on film, to increase contrast and produce a “bas-relief” effect. This gives the final image a three-dimensional appearance. No apparent loss of resolution results and no artificial amendment is required. To produce transparencies, a contact film positive is produced from the negative and the two are sandwiched in a slide mount with the appropriate amount of lateral displacement to give the effect. To produce prints, the film positive and the film negative are used to sequentially expose the same sheet of photographic paper prior to development to realize the effect. The bas-relief induced by the misregistration of the film plates causes apparent shadows and highlights that produce the apparent height that is seen in the enhanced images.     

Accelerating subsurface scattering using Cholesky factorization

In this paper we present a simplified subsurface scattering model that exploits a diffusion mechanism to provide a simpler solution to the transport equation. Our model is based on numerical analysis techniques that are amenable to Cholesky factorization. We treat the factorization as a precomputed scattering quantity which can be used to significantly speed up multiple scattering calculations as the global light source changes. On low resolution meshes, we have been able to achieve real-time solutions of the subsurface scattering while still maintaining good visual quality of the solution.     

The impact of zeta potential on the physical properties of ferric--NOM flocs

The physical properties of natural organic matter (NOM) flocs, such as size, growth rate, and strength, were investigated using a laser diffraction particle sizing device. Conditions were set such that varying carbon coagulant ratio and zeta potential could both be investigated. Results demonstrated a link between zeta potential and coagulation and flocculation performance, with the production of strong flocs and low residual concentrations when the zeta potential was minimized. The overall strength of the connection points within the floc were determined by a combination of steric interactions, polymer bridging, van der Waals forces, and electrostatic forces. Hence, both dose ratio and zeta potential are important in understanding floc properties. Floc growth was dominated by dose ratio, whereas the response to elevated shear was strongly related to zeta potential. The steady-state floc size was a combination of both factors. This allowed the continued development of a qualitative model in order to engineer optimal floc properties when coagulating NOM.