Application of the three-dimensional finite-difference time-domainmethod to the analysis of planar microstrip circuits

A direct three-dimensional finite-difference time-domain (FDTD) method is applied to the full-wave analysis of various microstrip structures. The method is shown to be an efficient tool for modeling complicated microstrip circuit components and microstrip antennas. From the time-domain results the input impedance of a line-fed rectangular patch antenna and the frequency-dependent scattering parameters of a low-pass filter and a branch-line coupler are calculated. These circuits were fabricated and the measurements made on them are compared with the FDTD results and shown to be in good agreement     

Robust Spiral Tool-Path Generation for Arbitrary Pockets

When converting CC data (cutter contact point data) into CL data (cutter location data) for tool-path generation for arbitrary pockets, overcut avoidance plays an important role in CNC pocket milling. Complicated calculations of the self-intersections in the offset of spiral cutting is one of the main considerations in some algorithms. This study presents a quasi-offset method to solve complicated self-intersection calculations. Instead of using offsetting methods, the proposed method uses the location points and their track types to generate the son loop. The proposed quasi-offset method also makes it much easier to generate spiral tool paths.     

Effect of temperature cycling on joint strength of PbSn and AuSnsolders in laser packages

The effect of temperature cycle testing on the joint strength of PbSn and AuSn solders in laser diode packages has been studied experimentally and numerically. Experimental results showed that the joint strength increased as the temperature cycle number increased initially, and then became steady after 400 cycles. The joint strengths of PbSn and AuSn solders increased about 40% to 20% after undergoing 500 temperature cycles, respectively. A finite-element method (FEM) analysis was performed on the calculation of joint strength variation of PbSn and AuSn solders in temperature cycling tests. The coupled thermal-elasticity-plasticity model was employed in the solidification and residual stresses calculation. Simulation results were in good agreement with the experimental measurements that the solder joint strength increased as the temperature cycle increased. Numerical results indicate that the increasing solder joint strength comes from the redistribution of the residual stresses within the solder during temperature cycling tests. The local yielding and the creep effects on the low melting temperature solders will make uniform the residual stresses distribution introduced in the solidification process and increasing the solder joint strength as the temperature cycle number increased. The result suggests that the FEM is an effective method for analyzing and predicting the solder joint strength in laser diode packages     

Post-weld-shift in dual-in-line laser package

The post-weld-shift (PWS) effect in laser welding for a dual-in-line package (DIP) with fiber pigtail to semiconductor laser connection has been studied experimentally and numerically. Experimental results show that the PWS of an optical component welded by a dual-beam laser system deforms and the welded component rotates counterclockwise as the difference of the energies between two laser beams increases. This indicates that the PWS in laser packaging can be minimized by properly controlling the laser beam-to-beam energy balance. A thermal-plasticity coupled finite-element model (FEM) has been also carried out on the analysis of the effect of PWS in laser packaging. Numerical results show that a PWS in the DIP may be introduced from an unbalanced distribution of residual stresses introduced from the solidification shrinkage. A satisfactory agreement between the experimental results and FEM calculations suggests that the FEM may provide an effective method for predicting the PWS in laser welding technique for optoelectronic packaging     

Postnatal High-Fat Diet Increases Liver Steatosis and Apoptosis Threatened by Prenatal Dexamethasone through the Oxidative Effect

The objective of this study was to investigate cellular apoptosis in prenatal glucocorticoid overexposure and a postnatal high fat diet in rats. Pregnant Sprague-Dawley rats at gestational days 14 to 21 were administered saline (vehicle) or dexamethasone and weaned onto either a normal fat diet or a high fat diet for 180 days; in total four experimental groups were designated, i.e., vehicle treated group (VEH), dexamethasone treated group (DEX), vehicle treated plus high-fat diet (VHF), and dexamethasone treated plus high-fat diet (DHF). Chronic effects of prenatal liver programming were assessed at postnatal day 180. The apoptotic pathways involved proteins were analyzed by Western blotting for their expressions. Apoptosis and liver steatosis were also examined by histology. We found that liver steatosis and apoptosis were increased in the DHF, DEX, and VHF treated groups, and that the DHF treated group was increased at higher levels than the DEX and VHF treated groups. The expression of leptin was decreased more in the DHF treated group than in the DEX and VHF treated groups. Decreased peroxisome proliferator-activated receptor-gamma coactivator 1α, phosphoinositide-3-kinase, manganese superoxide dismutase and increased malondialdehyde expression levels were seen in DHF treated group relative to the DEX treated group. The DHF treated group exhibited higher levels of oxidative stress, apoptosis and liver steatosis than the DEX treated group. These results indicate that the environment of high-fat diet plays an important role in the development of liver injury after prenatal stress.     

Combustion kinetic modeling using multispecies time histories in shock-tube oxidation of heptane

Recently, species time histories have been measured during n-heptane oxidation behind reflected shock waves [D.F. Davidson, Z. Hong, G.L. Pilla, A. Farooq, R.D. Cook, R.K. Hanson, Combust. Flame 157 (2010) 1899–1905]. The highly precise nature of these measurements is expected to impose critical constraints on chemical kinetic models of hydrocarbon combustion. In this paper, we apply the Method of Uncertainty Analysis using Polynomial Chaos Expansions (MUM-PCE) [D.A. Sheen, X. You, H. Wang, T. Løvås, Proc. Combust. Inst. 32 (2009) 535–542] to demonstrate how the multispecies measurement may be utilized beyond simple model validation. The results show that while an as-compiled, prior reaction model of n-alkane combustion can be accurate in its prediction of the detailed species profiles, the kinetic parameter uncertainty in the model remains to be too large to obtain a precise prediction of the data. Constraining the prior model against the species time histories within the measurement uncertainties led to notable improvements in the precision of model predictions against the species data as well as the global combustion properties considered. Lastly, we show that while the capability of the multispecies measurement presents a step-change in our precise knowledge of the chemical processes in hydrocarbon combustion, accurate data of global combustion properties are still necessary to predict fuel combustion.     

Modelling transfer of Listeria monocytogenes during slicing of 'gravad' salmon

Transfer of a rifampicin-resistant mutant of Listeria monocytogenes from an inoculated slicing blade to slices of 'gravad' salmon (Salmo salar), and from inoculated salmon fillet to the slicing machine and subsequently to slices of uninoculated fillet was studied. The effect of slicing temperature (0 degrees C, 10 degrees C and room temperature), inoculum level (approx. 3, 5 and 8 log CFU/blade), and attachment time of inoculum to blade (10 min and 2.5 h) were investigated and predictive models of the transfer were produced. In the tests of transfer from inoculated blade (5.9-9.0 log CFU/blade) initially 2.5-5.3 log CFU/g was present on the slices, slowly decreasing to an overall average decrease of 1.6+/-0.2 log CFU/g during slicing of 39 slices; the lowest reduction being 1.3 log CFU/g at 0 degrees C. In tests of transfer from contaminated salmon (7.6+/-0.1 log CFU/fillet) to uninoculated blade and further to uninoculated salmon, the reduction in number of L. monocytogenes in slices was 1.5 log CFU/g during slicing of 39 slices. For example 5.3+/-0.3 log CFU/g was transferred to second slice when the inoculum level was 8.4+/-0.4 log CFU/blade, but clearly (p<0.05) lower total number of L. monocytogenes were transferred to slices when the inoculum level was lower, the temperature was colder or the attachment time was longer. There was a progressive exponential reduction in the quantity of L. monocytogenes transferred and, based on statistical parameters, an exponential model (y=ae((-x/b))) fit the data from different test conditions and was suitable for predicting an expected number of L. monocytogenes on the salmon slices. Based on the predicted values, the logarithmic reduction in number of L. monocytogenes in slices was highest at room temperature with an inoculum level of 8.4+/-0.4 log CFU/blade (attachment time 10 min); the other test conditions differed significantly from this (p<0.05). Despite statistically significant differences, in all test conditions the number of bacteria were predicted to reduce quite rapidly (i.e. after slicing of the fourth fillet) to <1 log CFU/g, though this prediction was an extrapolation after 39 slices. The predictive models described herein can assist salmon processors and regulatory agencies in assessing cross-contamination from contaminated slicing machines to product and in designing risk management strategies.     

Modeling the impact of chlorine on the behavior of Listeria monocytogenes on ready-to-eat meats

Listeria monocytogenes (Lm) continues to pose a food safety hazard in ready-to-eat (RTE) meats due to potential cross-contamination. Chlorine is commonly used to sanitize processing equipment and utensils. However, Lm may survive the treatment and then contaminate food products. The objective of this study was to characterize the behavior of chlorine-exposed Lm on RTE ham during refrigerated storage. A two strain cocktail of Lm serotype 4b was pre-treated with chlorine (0, 25, and 50 ppm) for one hour, and then inoculated onto the surface of RTE ham to obtain an inoculum of about 3.0 log CFU/g. The inoculated ham samples were stored at 4, 8, and 16 °C, and Lm was enumerated periodically during the storage. The growth characteristics (lag time and growth rate) of Lm were estimated using the DMFit software. The results indicated that Lm growth was suppressed by the chlorine treatment. At 4 °C, the lag time of Lm with no (0 ppm) chlorine exposure (4.2 days) was shorter than those exposed to 25 ppm (5.4 days) and 50 ppm (6.8 days). The lag time decreased with the increase of temperature, e.g., at 25 ppm, the lag times were 5.2, 3.8 and 2.6 days for 4, 8 and 16 °C, respectively, and increased with the increase of chlorine concentration, e.g., at 16 °C, the lag times were 1.2, 2.6 and 4.0 days for 0, 25 and 50 ppm, respectively. However, growth rate increased with the increase of temperature and decreased with the increase of chlorine concentration. The lag time and growth rate as a function of chlorine concentration and temperature can be described using a modified Ratkowsky model and a modified Zwietering model, respectively. The results showed that the growth of Lm on RTE ham was delayed by pre-exposure to chlorine (at ≤50 ppm). The predictive models developed will contribute to microbial risk assessments of RTE meats.     

Growth characteristics of Listeria monocytogenes as affected by a native microflora in cooked ham under refrigerated and temperature abuse conditions

This study examined the growth characteristics of Listeria monocytogenes as affected by a native microflora in cooked ham at refrigerated and abuse temperatures. A five-strain mixture of L. monocytogenes and a native microflora, consisting of Brochothrix spp., isolated from cooked meat were inoculated alone (monocultured) or co-inoculated (co-cultured) onto cooked ham slices. The growth characteristics, lag phase duration (LPD, h), growth rate (GR, log₁₀ cfu/h), and maximum population density (MPD, log₁₀ cfu/g), of L. monocytogenes and the native microflora in vacuum-packed ham slices stored at 4, 6, 8, 10, and 12 °C for up to 5 weeks were determined. At 4-12 °C, the LPDs of co-cultured L. monocytogenes were not significantly different from those of monocultured L. monocytogenes in ham, indicating the LPDs of L. monocytogenes at 4-12 °C were not influenced by the presence of the native microflora. At 4-8 °C, the GRs of co-cultured L. monocytogenes (0.0114-0.0130 log₁₀ cfu/h) were statistically but marginally lower than those of monocultured L. monocytogenes (0.0132-0.0145 log₁₀ cfu/h), indicating the GRs of L. monocytogenes at 4-8 °C were reduced by the presence of the native microflora. The GRs of L. monocytogenes were reduced by 8-7% with the presence of the native microflora at 4-8 °C, whereas there was less influence of the native microflora on the GRs of L. monocytogenes at 10 and 12 °C. The MPDs of L. monocytogenes at 4-8 °C were also reduced by the presence of the native microflora. Data from this study provide additional information regarding the growth suppression of L. monocytogenes by the native microflora for assessing the survival and growth of L. monocytogenes in ready-to-eat meat products.     

Modeling the nonlinearity of superconducting strip transmissionlines

The use of the Ginzburg-Landau (GL) theory to predict the nonlinear behavior in a superconducting stripline resonator as a function of input current is reported. A method for calculating the nonlinear inductance and the fractional change in the resonant frequency (Δf/f) of a stripline resonator is presented. By solving the GL equations inside the superconducting strip, the spatial variation of the number density of superconducting electrons and, hence, the spatial variation of the magnetic penetration depth are obtained for different values of input current. First, an infinite parallel plate transmission line is considered where the one-dimensional GL equations are solved. The two-dimensional case of a stripline is then considered. Nonlinear inductances are calculated as functions of input current for different superconducting striplines. Comparisons of the calculated Δf/f with measurements for YBa₂Cu₃O_7-x stripline resonators show excellent agreement