The structure and organization of the human NPAT gene

Ataxia telangiectasia (AT) is an autosomal recessive gene disorder, and ATM, a housekeeping gene, has been identified as the gene responsible for AT. Recently we found that another housekeeping gene, NPAT, is located upstream of ATM on human chromosome 11. The two housekeeping genes are transcribed in opposite directions and share a 0.5-kb 5' flanking sequence. The structure and organization of NPAT were determined by direct sequencing of cosmid clones carrying the gene and by application of the long and accurate (LA)-PCR method to amplify regions encompassing the exon/intron boundaries and all of the exons. The gene spans at least 44 kb and consists of 18 exons and 17 introns. It has been suggested that AT heterozygotes have an increased risk of developing cancer, especially breast cancer in women. Frequently, loss of heterozygosity at loci on 11q22-q24 has been observed in DNA isolated from tumors of the breast, uterine cervix, and colon, perhaps suggesting the location of a tumor suppressor gene in 11q22-q24. For investigation of the role of NPAT in AT and these tumors with allelic loss of 11q22-q24, appropriate primer sequences and PCR conditions for amplification of all the NPAT exons from genomic DNA were determined. We previously reported that no recombinations are found among Atm, Npat, and Acat1 (acetoacetyl-CoA thiolase) loci as determined by fine genetic linkage mapping of the mouse AT region. The results of the LA-PCR analysis using NPAT- and ACAT-specific primers and human genomic DNA allowed us to map ACAT 12 kb centromeric to NPAT.     

A 16-Mb CMOS SRAM with a 2.3-μm2 single-bit-linememory cell

A novel architecture that enables fast write/read in poly-PMOS load or high-resistance polyload single-bit-line cells is developed. The architecture for write uses alternate twin word activation (ATWA) with bit-line pulsing. A dummy cell is used to obtain a reference voltage for reading. An excellent balance between a normal cell signal line and a dummy cell signal line is attained using balanced common data-line architecture. A newly developed self-bias-control (SBC) sense amplifier provides excellent stability and fast sensing performance for input voltages close to V_CC at a low power supply of 2.5 V. The single-bit-line architecture is incorporated in a 16-Mb SRAM, which was fabricated using 0.25-μm CMOS technology. The proposed single-bit-line architecture reduces the cell area to 2.3-μm² , which is two-thirds of a conventional two-bit-line cell with the same processes. The 16-Mb SRAM, a test chip for a 64-Mb SRAM, shows a 15-ns address access time and a 20-ns cycle time     

Multiple effects of CD4 CDR3-related peptide derivatives showing anti-HIV-1 activity on HIV-1 gp120 functions

The interaction of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120 with CD4 CDR3-related peptide derivatives showing anti-HIV-1 activity has been studied. Conformational changes in gp120, which could affect its interaction with CD4 and its shedding from virions, were detected by fluorescence spectrum analysis of tryptophan residues after addition of peptide representative of the CD4 CDR3-related region, but not the CD4 CDR2-related region. Interestingly, the addition of scrambled peptide, S1 (with altered amino acid sequence compared with the native CDR3-related peptide but unaltered overall composition), which we recently showed to have stronger anti-HIV-1 activity than the original CDR3-related peptide, had no effects on the conformational change in gp120 or on its interaction with CD4 and its shedding from HIV-1 virions. However, all of the CDR3-related peptides, including S1, showed blocking effects on the binding of antibodies against gp120 V3 loop and C-terminus regions. Thus, we concluded that there were at least two separable activities of the CDR3-related peptides in anti-HIV-1 activity, i.e. induction of conformational changes in gp120, which could affect its binding to CD4 and to gp41 (as observed in native CDR3-related peptides), and inactivation of V3 loop and C-terminus regions in gp120 (as observed in all of the CDR3-related peptides, including S1).     

Effect of polytetrafluoroethylene covering of Palmaz stents on the development of intimal hyperplasia in human iliac arteries

PURPOSE: The occurrence of neointimal hyperplasia within a stent may result in restenosis with recurrent symptoms of end-organ ischemia. This study evaluated the potential of a nonporous covering of a stent to function as a barrier to the formation of intrastent neointimal hyperplasia. MATERIALS AND METHODS: Twelve endovascular stent grafts were used to treat 12 high-risk patients with limb-threatening ischemia secondary to long-segment iliac artery occlusion. A 6-mm, thin-walled polytetrafluoroethylene graft was inserted and anchored to the common iliac artery with use of Palmaz stents. Each stent was covered by graft material over one-half of its length. Control angiograms obtained immediately after graft insertion were compared with follow-up angiograms obtained between 4 and 6 months after the initial procedure. On each angiogram, the region of the stent was magnified by 20x to permit computerized luminal diameter measurements. RESULTS: The mean luminal diameter within the stent was significantly greater on the covered (7.7 mm +/- 0.33 standard deviation) compared with the uncovered (6.7 mm +/- 0.85 standard deviation) portions (P < .01). CONCLUSIONS: Partially covered stents are a unique model for assessing the effects of an extrinsic stent covering on arterial healing and myointimal hyperplasia. These data suggest that a relatively nonporous covering of polytetrafluoroethylene may inhibit stent-related restenosis in iliac arteries.     

Simplified stable crack growth analyses of welded CT specimens—comparison study of GE/EPRI, reference stress and R6 methods

This paper describes some simplified stable crack growth analyses of two kinds of inhomogeneous CT specimens. The one is machined from a submerged are welded plate of a nuclear pressure vessel A533B Class 1 steel, while the other is machined from an electron-beam welded plate of the A533B Class 1 steel and a high strength HT80 steel. In both specimens, initial cracks are placed to be normal to the fusion line. The ratio of yield stresses of the weld metal and the base metal of the A533B Class 1 steel is about 1·15, while that of the HT80 and the A533B Class 1 steels is about 1·4.

The generation phase crack growth analyses using the GE/EPRI and the reference stress methods are performed, calculating an applied load (P) and the J-value, while the application phase analyses of analyses using the R6 method are performed to calculate the maximum value of the applied load (P_max). Finally, some modification procedures of the three simplified estimation schemes are discussed in order to apply them to inhomogeneous material regimes.     

Automaticity in conversations: A reexamination of the mindlessness hypothesis.

The automatic processing of an ordinary conversation, or what has been called mindlessness (Langer, Blank, & Chanowitz, 1978), implies that comprehension of conversation is based almost entirely on generic knowledge. As a consequence, memory of what was said will contain typical events but virtually no idiosyncratic information. To test this prediction, we conducted a field experiment in which college students were individually approached and asked to perform a small favor. Attention was either induced or allowed to operate naturally. Specifically, half of the students were warned beforehand of the forthcoming request and told to pay close attention and half were not forewarned. As expected, regardless of the condition, the generic features of the request were well remembered. On the other hand, memory for idiosyncratic information, namely, specific words used in the request, decreased when the structure of the conversation conformed to cultural conventions and when attention to the request was not induced. Findings cast doubt on the perfect automaticity suggested by the mindlessness hypothesis (Langer et al., 1978). (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Ozone treatment of water-soluble polymers. III. Ozone degradation of polyacrylamide in water

Polyacrylamide (MW 350,000) was ozonized at pH 2 and pH 10 buffer solution. There was little ozonization at pH 2, but at pH 10 the COD and the viscosity of the solution decreased upon ozonization, and a linear relationship existed between the ozone consumed and the number of breaks calculated from the viscosity. This relationship apparently indicated that 45 molecules ozone were consumed for one cleavage of the polymer chain. Such random cleavage was confirmed also by the observation of molecular weight distribution by means of gel filtration chromatography. The amide group decreased scarcely by the ozonization, while a small amount of aldehyde was observed in the ozonized solution. No remarkable change was observed in the IR spectra of the ozonized sample, except for a weak absorption band at 1725 cm^?1 which arose from the carbonyl of aldehyde or ketone. Although no variation was observed in the ¹³C NMR spectra either, a strong absorption peak at 266 nm appeared in the UV spectra of the ozonized solution and increased with the ozonization time. This phenomenon was presumed to be due to the formation of a certain ring structure between the amide group and the small amount of ketone produced in the main chain. However, the details of the ozonization mechanism could not be ascertained.     

Kinetic analysis of crystallization of zeolite beta synthesized by direct heating

To quantitatively investigate the initial crystallization of zeolite beta synthesized by direct heating, the extent of the reaction was precisely evaluated by X-ray diffraction measurements and Rietveld structural refinement, and a kinetic analysis of crystallization was performed using the Avrami-Erofe'ev equation. The activation energy for crystallization was lower than that for hydrothermal synthesis. Reaction and synthesis time curves revealed that the initial zeolite beta crystallization consisted of three stages. The first was an induction period with nucleation by the generation of building units and the formation of an initial coordinated structure. The second stage was crystal growth by a diffusion-controlled reaction, and the third stage involved slowing down of crystallization by the limitation of dehydrocondensation. These stages could be analyzed by calculation of the rate constant and Avrami exponent for each stage.