Rice β-Glucosidase 4 (Os1βGlu4) Regulates the Hull Pigmentation via Accumulation of Salicylic Acid

Salicylic acid (SA) is a stress hormone synthesized in phenylalanine ammonia-lyase (PAL) and the branching acid pathway. SA has two interconvertible forms in plants: SAG (SA O-β-glucoside) and SA (free form). The molecular mechanism of conversion of SA to SAG had been reported previously. However, which genes regulate SAG to SA remained unknown. Here, we report a cytoplasmic β-glucosidase (β-Glu) which participates in the SA pathway and is involved in the brown hull pigmentation in rice grain. In the current study, an EMS-generated mutant brown hull 1 (bh1) displayed decreased contents of SA in hulls, a lower photosynthesis rate, and high-temperature sensitivity compared to the wild type (WT). A plaque-like phenotype (brown pigmentation) was present on the hulls of bh1, which causes a significant decrease in the seed setting rate. Genetic analysis revealed a mutation in LOC_Os01g67220, which encodes a cytoplasmic Os1βGlu4. The knock-out lines displayed the phenotype of brown pigmentation on hulls and decreased seed setting rate comparable with bh1. Overexpression and complementation lines of Os1βGlu4 restored the phenotype of hulls and normal seed setting rate comparable with WT. Subcellular localization revealed that the protein of Os1βGlu4 was localized in the cytoplasm. In contrast to WT, bh1 could not hydrolyze SAG into SA in vivo. Together, our results revealed the novel role of Os1βGlu4 in the accumulation of flavonoids in hulls by regulating the level of free SA in the cellular pool.     

VPR-Bench: An Open-Source Visual Place Recognition Evaluation Framework with Quantifiable Viewpoint and Appearance Change

International Journal of Computer Vision - Visual place recognition (VPR) is the process of recognising a previously visited place using visual information, often under varying appearance...     

A wireless Web for creating and sharing personal content through handsets

Mobile handsets have evolved from voice transmission devices to sophisticated digital assistants permanently connected to the Internet. These handsets now include hardware attachments that let users capture images and video and accurately track handsets' positions. These new features combined with the terminals' data transmission capabilities support a rich multimedia interaction model that enables users to share digital assets directly from their mobile devices. We call this model the Personalized Group Wide Web. PGWW aims to provide a flexible, simple, and efficient mechanism to enable next-generation cell phone users to share their digital assets. Increasingly sophisticated handheld devices provide an appropriate infrastructure to host a highly dynamic wireless WWW variant. This variant uses a flexible middleware infrastructure to overcome handset heterogeneity and resource limitations.     

Non‐Newtonian squashed flow simulation across Darcy‐Forchheimer sensor

The current mathematical formulation is dedicated to investigate the Darcy‐Forchheimer boundary layer–squeezed hydromagnetic flow of a Casson fluid passing through a sensor surface. The flow phenomenon is occurring in a locally free stream under the combined sway of heat generation and thermic radiation. The energy equation is deliberated with the assistance of Cattaneo‐Christov theory rather than using Fourier's law for conduction of heat. Here, the thermic conductivity is being presumed as a function of temperature. The governing mathematical structure consists of highly nonlinear terms, so a set of regulatory parameters is being accomplished to attain the unpretentious dimensionless equations. This nondimensional structure is then treated numerically to attain the nearly converging results. The significance of substantial parameters such as magnetic factor, radiation parameter, Casson fluid parameter, heat origination, and thermal relaxation time on the flow phenomenon is estimated and presented graphically. Besides this, the factors of engineering interest like the Prandtl number and squeezed flow index with vacillating thermic conductivity have strong effects on the flow behavior of the fluid. It is observed that the magnetic effect causes an expansion in the velocity curve while a reduction is found for squeezed flow index parameter.     

Boosted hydrogen evolution activity from Sr doped ZnO/CNTs nanocomposite as visible light driven photocatalyst

CNTs were decorated onto Sr doped ZnO nanoparticles to construct an efficient photocatalyst via a facile sol-gel method. The as-fabricated Sr doped ZnO/CNTs with recyclability exhibits Sr and CNTs content dependent hydrogen evolution activit under visible light illumination. The Sr doped ZnO/CNTs photocatalyst shows the highest hydrogen evolution rate of 2732.2 μmolh^?1g^?1, which is 33.7 and 2.83 times higher than pure ZnO and Sr doped ZnO photocatalysts, respectively. The improved hydrogen evolution activity of Sr doped ZnO/CNTs is primarily assigned to high surface area, Sr doping and construction of heterojunction, which can extend the light absorption, decrease the optical band gap and improve the charge separation. Moreover, the underlying photocatalytic mechanism is proposed on the basis of Mott-Schottky study and explains the interfacial charge transfer process from ZnO to CNTs and Sr. This work open new strategies to synthesize CNTs based nanocomposite for hydrogen evolution.     

Creatinine-based non-phospholipid vesicular carrier for improved oral bioavailability of Azithromycin

Context: Novel, safe, efficient and cost effective nano-carriers from renewable resources have got greater interest for enhancing solubility and bioavailability of hydrophobic dugs.

Objectives: This study reports the synthesis of a novel biocompatible non-phospholipid human metabolite "Creatinine" based niosomal delivery system for Azithromycin improved oral bioavailability.

Methods: Synthesized surfactant was characterized through spectroscopic and spectrometric techniques and then the potential for niosomal vesicle formation was evaluated using Azithromycin as model drug. Drug loaded vesicles were characterized for size, polydispersity index (PDI), shape, drug encapsulation efficiency (EE), in vitro release and drug–excipient interaction using zetasizer, atomic force microscope (AFM), LC–MS/MS and FTIR. The biocompatibility of surfactant was investigated through cells cytotoxicity, blood hemolysis and acute toxicity. Azithromycin encapsulated in niosomes was investigated for in vivo bioavailability in rabbits.

Results: The vesicles were spherical with 247?±?4.67?nm diameter hosting 73.29?±?3.51% of the drug. Surfactant was nontoxic against cell cultures and caused 5.80?±?0.51% hemolysis at 1000?µg/mL. It was also found safe in mice up to 2.5?g/kg body weight. Synthesized surfactant based niosomal vesicles revealed enhanced oral bioavailability of Azithromycin in rabbits.

Conclusions: The results of the present study confirm that the novel surfactant is highly biocompatible and the niosomal vesicles can be efficiently used for improving the oral bioavailability of poor water soluble drugs.     

A survey on search results diversification techniques

The quantity of information placed on the web has been greater than before and is increasing rapidly day by day. Searching through the huge amount of data and finding the most relevant and useful result set involves searching, ranking, and presenting the results. Most of the users probe into the top few results and neglect the rest. In order to increase user’s satisfaction, the presented result set should not only be relevant to the search topic, but should also present a variety of perspectives, that is, the results should be different from one another. The effectiveness of web search and the satisfaction of users can be enhanced through providing various results of a search query in a certain order of relevance and concern. The technique used to avoid presenting similar, though relevant, results to the user is known as a diversification of search results. This article presents a survey of the approaches used for search result diversification. To this end, this article not only provides a technical survey of existing diversification techniques, but also presents a taxonomy of diversification algorithms with respect to the types of search queries.     

Solution of Algebraic Lyapunov Equation on Positive-Definite Hermitian Matrices by Using Extended Hamiltonian Algorithm

This communique is opted to study the approximate solution of the Algebraic Lyapunov equation on the manifold of positive-definite Hermitian matrices. We choose the geodesic distance between -AHX - XA and P as the cost function, and put forward the Extended Hamiltonian algorithm (EHA) and Natural gradient algorithm (NGA) for the solution. Finally, several numerical experiments give you an idea about the effectiveness of the proposed algorithms. We also show the comparison between these two algorithms EHA and NGA. Obtained results are provided and analyzed graphically. We also conclude that the extended Hamiltonian algorithm has better convergence speed than the natural gradient algorithm, whereas the trajectory of the solution matrix is optimal in case of Natural gradient algorithm (NGA) as compared to Extended Hamiltonian Algorithm (EHA). The aim of this paper is to show that the Extended Hamiltonian algorithm (EHA) has superior convergence properties as compared to Natural gradient algorithm (NGA). Upto the best of author’s knowledge, no approximate solution of the Algebraic Lyapunov equation on the manifold of positive-definite Hermitian matrices is found so far in the literature.     

Input Selection of Wavelet-Coupled Neural Network Models for Rainfall-Runoff Modelling

Water Resources Management - The use of wavelet-coupled data-driven models is increasing in the field of hydrological modelling. However, wavelet-coupled artificial neural network (ANN) models...