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121.
RA Alvarez AJ Ghalayini P Xu A Hardcastle S Bhattacharya PN Rao MJ Pettenati RE Anderson W Baehr 《Canadian Metallurgical Quarterly》1995,29(1):53-61
Defects in the Drosophila norpA (no receptor potential A) gene encoding a phosphoinositide-specific phospholipase C (PLC) block invertebrate phototransduction and lead to retinal degeneration. The mammalian homolog, PLCB4, is expressed in rat brain, bovine cerebellum, and the bovine retina in several splice variants. To determine a possible role of PLCB4 gene defects in human disease, we isolated several overlapping cDNA clones from a human retina library. The composite cDNA sequence predicts a human PLC beta 4 polypeptide of 1022 amino acid residues (MW 117,000). This PLC beta 4 variant lacks a 165-amino-acid N-terminal domain characteristic for the rat brain isoforms, but has a distinct putative exon 1 unique for human and bovine retina isoforms. A PLC beta 4 monospecific antibody detected a major (130 kDa) and a minor (160 kDa) isoform in retina homogenates. Somatic cell hybrids and deletion panels were used to localize the PCLB4 gene to the short arm of chromosome 20. The gene was further sublocalized to 20p12 by fluorescence in situ hybridization. 相似文献
122.
运用炸药混装车技术开采堆石坝级配料 总被引:2,自引:0,他引:2
葛洲坝集团公司成功地将乳化炸药混装车技术引入三峡水电工程的土石方开挖中,取得了良好的效益,在国内还未曾有人将该技术应用于堆石坝级配料开采。本文结合清江水布垭工程爆破试验,对运用混装车技术开采堆石料进行了研究。事实证明:把混装车技术引入堆石坝级配料开采可大大提高开采强度,降低工程成本;增加细颗粒石料,优化堆石料级配;爆破效果良好,安全可靠。 相似文献
123.
用Nardini-Brebbia边界元法计算了动载荷下的应力强度因子,与解析解及有限元解相比较,效果较好。最后对计算结果进行了分析讨论。 相似文献
124.
RL Richards M Rao NM Wassef GM Glenn SW Rothwell CR Alving 《Canadian Metallurgical Quarterly》1998,66(6):2859-2865
Encapsulation of soluble protein antigens in liposomes was previously shown to result in processing of antigen via the major histocompatibility complex class I pathway, as evidenced by costaining of the trans-Golgi region of murine bone marrow-derived macrophages (BMs) by fluorophore-labeled liposomal antigen and by a trans-Golgi-specific fluorescent lipid. Evidence is presented here that free or liposome-encapsulated RTS,S, a particulate malaria antigen consisting of hepatitis B particles coexpressed with epitopes from the Plasmodium falciparum circumsporozoite protein, also was localized in the trans-Golgi after incubation with BMs, suggesting processing by the class I pathway. An in vivo cytotoxic T-lymphocyte (CTL) response was detected, however, only after immunization with RTS,S encapsulated in liposomes containing lipid A and not after immunization with free RTS,S or with RTS,S encapsulated in liposomes lacking lipid A. Therefore, intracellular delivery of antigen containing CTL epitopes to the Golgi of BMs does not necessarily result in a CTL response in vivo unless an additional adjuvant, such as liposomes containing lipid A, is utilized. Encapsulation of RTS,S in liposomes containing monophosphoryl lipid A (MPL) resulted in a dose-dependent enhancement of the NANP-specific immunoglobulin G (IgG) antibody response compared to that of free RTS,S. The IgG1 and IgG2a subclasses predominated after immunization with RTS,S encapsulated in liposomes containing MPL. These results demonstrate that encapsulation of a lipid-containing particulate antigen, such as RTS, S, in liposomes containing lipid A can enhance both humoral and cellular immune responses. 相似文献
125.
N Ram MV Rao L Prabhu CS Nirodi S Sultana PG Vatsala G Padmanaban 《Canadian Metallurgical Quarterly》1995,317(1):39-45
Polymeric films were deposited from hydroxyethylmethacrylate (HEMA) plasma on non-woven poly(butyleneterephtalate) (PBT) filter materials. To test the effect of deposition conditions on surface properties, film were deposited using a constant monomer flow rate and a discharge power ranging from 40-100 W. Surface composition and surface energetics were evaluated by Electron Spectroscopy for Chemical Analysis (ESCA) and contact angle measurement, respectively. Albumin (Alb) and fibrinogen (Fg) adsorption from single protein solutions to the plasma-coated filters was measured. Results illustrate the marked effects of the deposition condition on the surface composition, the surface field of forces, and the protein adsorption behavior. The latter is modeled by the application of the Good-van Oss-Chaudhury theory of Lewis acid-base contribution to interfacial energetics. Materials endowed with widely different properties are obtained from the same monomer and different deposition conditions, a result that must be taken into account both in the production step, to assure constant quality, and in the development of specifically tailored materials. 相似文献
126.
A Maximum Likelihood Stereo Algorithm 总被引:8,自引:0,他引:8
Ingemar J. Cox Sunita L. Hingorani Satish B. Rao Bruce M. Maggs 《Computer Vision and Image Understanding》1996,63(3):542-567
A stereo algorithm is presented that optimizes a maximum likelihood cost function. The maximum likelihood cost function assumes that corresponding features in the left and right images are normally distributed about a common true value and consists of a weighted squared error term if two features are matched or a (fixed) cost if a feature is determined to be occluded. The stereo algorithm finds the set of correspondences that maximize the cost function subject to ordering and uniqueness constraints. The stereo algorithm is independent of the matching primitives. However, for the experiments described in this paper, matching is performed on the $cf4$individual pixel intensities.$cf3$ Contrary to popular belief, the pixel-based stereo appears to be robust for a variety of images. It also has the advantages of (i) providing adensedisparity map, (ii) requiringnofeature extraction, and (iii)avoidingthe adaptive windowing problem of area-based correlation methods. Because feature extraction and windowing are unnecessary, a very fast implementation is possible. Experimental results reveal that good stereo correspondences can be found using only ordering and uniqueness constraints, i.e., withoutlocalsmoothness constraints. However, it is shown that the original maximum likelihood stereo algorithm exhibits multiple global minima. The dynamic programming algorithm is guaranteed to find one, but not necessarily the same one for each epipolar scanline, causing erroneous correspondences which are visible as small local differences between neighboring scanlines. Traditionally, regularization, which modifies the original cost function, has been applied to the problem of multiple global minima. We developed several variants of the algorithm that avoid classical regularization while imposing several global cohesiveness constraints. We believe this is a novel approach that has the advantage of guaranteeing that solutions minimize the original cost function and preserve discontinuities. The constraints are based on minimizing the total number of horizontal and/or vertical discontinuities along and/or between adjacent epipolar lines, and local smoothing is avoided. Experiments reveal that minimizing the sum of the horizontal and vertical discontinuities provides the most accurate results. A high percentage of correct matches and very little smearing of depth discontinuities are obtained. An alternative to imposing cohesiveness constraints to reduce the correspondence ambiguities is to use more than two cameras. We therefore extend the two camera maximum likelihood toNcameras. TheN-camera stereo algorithm determines the “best” set of correspondences between a given pair of cameras, referred to as the principal cameras. Knowledge of the relative positions of the cameras allows the 3D point hypothesized by an assumed correspondence of two features in the principal pair to be projected onto the image plane of the remainingN− 2 cameras. TheseN− 2 points are then used to verify proposed matches. Not only does the algorithm explicitly model occlusion between features of the principal pair, but the possibility of occlusions in theN− 2 additional views is also modeled. Previous work did not model this occlusion process, the benefits and importance of which are experimentally verified. Like other multiframe stereo algorithms, the computational and memory costs of this approach increase linearly with each additional view. Experimental results are shown for two outdoor scenes. It is clearly demonstrated that the number of correspondence errors is significantly reduced as the number of views/cameras is increased. 相似文献
127.
阐述了EDTA清洗锅炉的基本原理,低浓度清洗新建炉的工艺、方法及控制要点,EDTA低浓度清洗与协调EDTA清洗的经济性比较,为新建炉EDTA低浓度清洗探索了方向。 相似文献
128.
Large internal strains and stresses can be produced by low temperature implantation over small distances from the free surface in a thick substrate. These are typically non-uniform and have large composition gradients. In dilute bcc solutions, containing unclustered interstitial implants, the residual macroscopic strains may be treated as isotropic. The calculation of residual strain (or stress) is based upon anisotropic elasticity theory and internal stress is given in terms of the dipole tensor for individual defects in single crystal films. In a completely elastic zone, forces act to maintain a rigid outside surface and cause the strain distribution to be zero along directions parallel to the free surface. This produces a strain magnification along the perpendicular direction from Poisson contractions. If the implanted zone is completely relaxed by plastic deformation, the strains are described by the free expansion strains due to both implants and lattice damage. There is no angular dependence of the free expansion strain in this extreme condition. One can determine whether a zone is completely elastic, completely relaxed by plastic deformation, or in some intermediate state from plots of strain against sin2, where is the angle of tilt relative to the surface normal. These results may be obtained from X-ray Bragg intensity data by measuring shifts and line broadening from (hkl) planes at different tilt angles. Theoretical results are given for both single crystal and polycrystalline materials in terms of residual strain and stress. 相似文献
129.
Yu Zhi Shi Xiuzhi Zhou Jian Rao Dijun Chen Xin Dong Wenming Miao Xiaohu Ipangelwa Timo 《Engineering with Computers》2021,37(2):991-1006
Engineering with Computers - The indirect and accurate determination of blast-induced rock movement has important significance in the reduction of ore loss and dilution and in the protection of... 相似文献
130.
R.H. Laskar D. Chakrabarty F.A. Talukdar K. Sreenivasa Rao K. Banerjee 《Applied Soft Computing》2012,12(11):3332-3342
In this paper, we present a comparative analysis of artificial neural networks (ANNs) and Gaussian mixture models (GMMs) for design of voice conversion system using line spectral frequencies (LSFs) as feature vectors. Both the ANN and GMM based models are explored to capture nonlinear mapping functions for modifying the vocal tract characteristics of a source speaker according to a desired target speaker. The LSFs are used to represent the vocal tract transfer function of a particular speaker. Mapping of the intonation patterns (pitch contour) is carried out using a codebook based model at segmental level. The energy profile of the signal is modified using a fixed scaling factor defined between the source and target speakers at the segmental level. Two different methods for residual modification such as residual copying and residual selection methods are used to generate the target residual signal. The performance of ANN and GMM based voice conversion (VC) system are conducted using subjective and objective measures. The results indicate that the proposed ANN-based model using LSFs feature set may be used as an alternative to state-of-the-art GMM-based models used to design a voice conversion system. 相似文献