Microarchitectural innovations: boosting microprocessor performancebeyond semiconductor technology scaling

Semiconductor technology scaling provides faster and more plentiful transistors to build microprocessors, and applications continue to drive the demand for more powerful microprocessors. Weaving the raw semiconductor material into a microprocessor that offers the performance needed by modern and future applications is the role of computer architecture. This paper overviews some of the microarchitectural techniques that empower modem high-performance microprocessors. The techniques are classified into: 1) techniques meant to increase the concurrency in instruction processing, while maintaining the appearance of sequential processing and 2) techniques that exploit program behavior. The first category includes pipelining, superscalar execution, out-of-order execution, register renaming, and techniques to overlap memory-accessing instructions. The second category includes memory hierarchies, branch predictors, trace caches, and memory-dependence predictors. The paper also discusses microarchitectural techniques likely to be used in future microprocessors, including data value speculation and instruction reuse, microarchitectures with multiple sequencers and thread-level speculation, and microarchitectural techniques for tackling the problems of power consumption and reliability     

A boolean spider monkey optimization based energy efficient clustering approach for WSNs

Wireless sensor network (WSN) consists of densely distributed nodes that are deployed to observe and react to events within the sensor field. In WSNs, energy management and network lifetime optimization are major issues in the designing of cluster-based routing protocols. Clustering is an efficient data gathering technique that effectively reduces the energy consumption by organizing nodes into groups. However, in clustering protocols, cluster heads (CHs) bear additional load for coordinating various activities within the cluster. Improper selection of CHs causes increased energy consumption and also degrades the performance of WSN. Therefore, proper CH selection and their load balancing using efficient routing protocol is a critical aspect for long run operation of WSN. Clustering a network with proper load balancing is an NP-hard problem. To solve such problems having vast search area, optimization algorithm is the preeminent possible solution. Spider monkey optimization (SMO) is a relatively new nature inspired evolutionary algorithm based on the foraging behaviour of spider monkeys. It has proved its worth for benchmark functions optimization and antenna design problems. In this paper, SMO based threshold-sensitive energy-efficient clustering protocol is proposed to prolong network lifetime with an intend to extend the stability period of the network. Dual-hop communication between CHs and BS is utilized to achieve load balancing of distant CHs and energy minimization. The results demonstrate that the proposed protocol significantly outperforms existing protocols in terms of energy consumption, system lifetime and stability period.     

Bacillus thuringiensis enzyme-digested delta endotoxin: effect on cultured insect cells

Cells from three insect cell lines responded to the enzyme-digested delta endotoxin of Bacillus thuringiensis with swelling, lysis, and vesicle formation. Sufficient toxin was taken up in 1 minute to cause half-maximal cell damage. Cytoxic activity was neutralized by specific antiserum to the endotoxin.     

Crystal structure of the zinc-dependent beta-lactamase from Bacillus cereus at 1.9 A resolution: binuclear active site with features of a mononuclear enzyme

The structure of the zinc-dependent beta-lactamase II from Bacillus cereus has been determined at 1.9 A resolution in a crystal form with two molecules in the asymmetric unit and 400 waters (space group P3121; Rcryst = 20.8%). The active site contains two zinc ions: Zn1 is tightly coordinated by His86, His88, and His149, while Zn2 is loosely coordinated by Asp90, Cys168, and His210. A water molecule (W1) lies between the two zinc ions but is significantly closer to Zn1 and at a distance of only 1.9 A is effectively a hydroxide moiety and a potential, preactivated nucleophile. In fact, Asp90 bridges W1 to Zn2, and its location is thus distinct from that of the bridging water molecules in the binuclear zinc peptidases or other binuclear zinc hydrolases. Modeling of penicillin, cephalosporin, and carbapenem binding shows that all are readily accommodated within the shallow active site cleft of the enzyme, and the Zn1-bound hydroxide is ideally located for nucleophilic attack at the beta-lactam carbonyl. This enzyme also functions with only one zinc ion present. The Zn1-Zn2 distances differ in the two independent molecules in the crystal (3.9 and 4.4 A), yet the Zn1-W1 distances are both 1.9 A, arguing against involvement of Zn2 in W1 activation. The role of Zn2 is unclear, but the B. cereus enzyme may be an evolutionary intermediate between the mono- and bizinc metallo-beta-lactamases. The broad specificity of this enzyme, together with the increasing prevalence of zinc-dependent metallo-beta-lactamases, poses a real clinical threat, and this structure provides a basis for understanding its mechanism and designing inhibitors.     

Analysis of ecdysteroid action in Malacosoma disstria cells: cloning selected regions of E75- and MHR3-like genes

IPRI-MD-66 (MD-66) cells respond to 20-hydroxyecdysone (20E, 4 x 10(-6) M) in the medium by producing cytoplasmic extensions, clumping and attaching themselves to the substrate. These morphological changes are at a maximum by 6 days post treatment. Degenerate oligonucleotides, designed on the basis of conserved amino acid sequences in the DNA and ligand binding regions of the members of the steroid hormone receptor superfamily, were used in RNA-PCR to isolate two cDNA fragments, Malacosoma disstria hormone receptor 2 (MdHR2) and Malacosoma disstria hormone receptor 3 (MdHR3) from the MD-66 cells. Comparison of deduced amino acid sequences of these cDNA fragments with the members of the steroid hormone receptor superfamily showed that MdHR2 is most closely related to E75 proteins of Manduca sexta, Galleria mellonella and Drosophila melanogaster. The MdHR3 is most closely related to Manduca hormone receptor 3 (MHR3), Galleria hormone receptor 3 (GHR3) and Drosophila hormone receptor 3 (DHR3) proteins. At a concentration of 4 x 10(-6) M, 20E induces the expression of MdHR2 and MdHR3 beginning at 3 h, reaching maximum levels in 12 h and declining in 24 h. MdHR2 binds to a 2.5 kb mRNA, whereas MdHR3 binds to a 4.5 kb mRNA. Based on sequence similarity, RNA size and ecdysone inducibility, we conclude that these cDNA fragments, cloned from MD-66 cells, are regions of E75- (MdHR2) and MHR3- (MdHR3) like genes.     

Scalable shared-memory multiprocessor architectures

Directory-based and bus-based cache coherence schemes are defined and described. Directory-based schemes can be classified as centralized or distributed. Both categories support local caches to improve processor performance and reduce traffic in the interconnection. Schemes using presence flags, B pointers, and linked lists are discussed. Bus-based systems provide uniform memory access to all processors. This memory organization allows a simpler programming model, making it easier to develop new parallel applications or to move existing applications from a uniprocessor to a parallel system. Two architectural variations of bus-based systems are described: multiple-bus and hierarchical architectures     

Corrosion resistance of multilayer coatings of nanolayered Cr/Ni electrodeposited from Cr(III)-Ni(II) bath

In this study the corrosion resistance of chromium and nickel single layers and multilayer coatings of nanolayered Cr/Ni, electrodeposited from Cr(III)-Ni(II) baths on low carbon steel substrates, has been studied. The coatings were electrodeposited from a bath using pulse current and modulated agitation. The total thickness of single layer and multilayer coatings was fixed at 5 μm and multilayer coatings with different modulation wavelengths and Cr to Ni thickness ratio were electrodeposited. Corrosion behavior of coatings was then studied by using potentiodynamic polarization test and electrochemical impedance spectroscopy in 0.1 M H₂SO₄. The results showed that Cr and Ni single layers had low corrosion resistance due to the presence of surface cracks and pores, respectively. On the other hand, optimized 20 nm Cr/50 nm Ni multilayer deposition significantly improved corrosion resistance.