Nonliposomal approach--a study of preparation of egg albumin nanospheres containing amphotericin-B

The stability of liposomes after introduction into the body is presently being discussed and needs thorough understanding. Hence, as a nonliposomal approach, egg albumin nanospheres were prepared by the pH-coacervation method, and a preliminary study was carried out of the influence of process variables on the size and shape of nanospheres by changing the pH of the albumin solution, concentration of albumin solution, and volume of cross-linking agent. The batch prepared with an albumin medium of pH 9, 2% concentration, and 100 μl of 4% glutaraldehyde-ethanol solution was found to have a spherical uniform shape with an average size of 497.6 nm. The ideal batch was loaded with the systemic antifungal drug amphotericin-B. Drug-loaded nanospheres were evaluated to study their in vitro release. They were found to exhibit a biphasic pattern with a cumulative percentage release of 97.7%.     

Ab initio modelling of basal plane oxidation of graphenes and implications for modelling char combustion

Ab initio calculations have been performed to determine the energetics of oxygen atoms adsorbed onto graphene planes and the possible reaction path extracting carbon atoms in the form of carbon monoxide. From the energetics it is confirmed that this reaction path will not significantly contribute to the gasification of well ordered carbonaceous chars. Modelling results which explore this limit are presented.     

Network architecture and communication protocols for mobile computing

We discuss the design and implementation of network and transport layer protocols for mobile networking. The network architecture used is unique in that it separates the mobile network(s) from fixed networks and provides connectivity between the two via special gateways. These gateways split all transport connections and provide QoS guarantees to mobile users for all their open connections. We provide summaries of our protocols and discuss possible improvements.     

Applicability of selected probability distributions to the number of authors per article in theoretical population genetics

Recently scientists have investigated what statistical distributions can be used to describe the distribution of the number of authors per article.Ajiferuke has undertaken the most comprehensive study of this problem. He has found that by and large the Inverse Gaussian-Poisson distribution could describe most properly the observed authorship distributions. However, it is well known that this distribution is rather intricate, soRousseau tried to fit some simple one-parameter distributions to the number of authors of LIS articles. He has found that the geometric and the truncated Poisson distribution adequately describe these authorship data sets. The main purpose of the present paper is to continue these investigations and to analyse and test the viability of simple statistical distributions. As to (sub)fields where the single author dominates the results ofRousseau were corroborated: the truncated Poisson and the geometric distribution give often adequate fits to describe the number of authors. The Lotka distribution should be rejected. The truncated binomial distribution and the truncated negative binomial were investigated as well. However, it is not clear whether they are acceptable candidates.     

Methods for calculating point-to-point congestion in switched communication networks

This paper presents two simple algorithms for computing the point-to-point congestion probability in a switched communication network. Methods are straightforward and can easily be computerized. Examples illustrate the methods.     

An optimal model for representing the kinetics of growth and product formation by Lactobacillus rhamnosus on multiple substrates

A comprehensive model was developed to simulate Lactobacillus rhamnosus growth on a medium containing multiple limiting carbon sources. The strategy of optimizing specific growth rate to predict growth on multiple substrates was demonstrated. The model predictions were based on parameters obtained from L. rhamnosus growth on individual substrates. The model was able to simulate the growth, substrate consumption, product formation and specific growth rate profiles of L. rhamnosus accurately. The model prediction that co-metabolism of glucose and pyruvate enhances growth rate of and flavor production by the bacterium was experimentally verified.     

Formation of neutral molecules of potential stellar interest by neutralisation of negative ions in a mass spectrometer. The application of experiment and molecular modelling in concert

This paper is a modified version of a lecture which describes the synthesis, structure and reactivity of some neutral molecules of stellar significance. The neutrals are formed in the collision cell of a mass spectrometer following vertical Franck-Condon one electron oxidation of anions of known bond connectivity. Neutrals are characterised by conversion to positive ions and by extensive theoretical studies at the CCSD(T)/aug-cc-pVDZ//B3LYP/6-31G(d) level of theory. Four systems are considered in detail, viz (i) the formation of linear C(4) and its conversion to the rhombus C(4), (ii) linear C(5) and the atom scrambling of this system when energised, (iii) the stable cumulene oxide CCCCCO, and (iv) the elusive species O(2)C-CO. This paper is not intended to be a review of interstellar chemistry: examples are selected from our own work in this area.     

Pullout Behavior of Granular Pile-Anchors in Expansive Clay Beds In Situ

Granular pile anchors (GPA) are one of the recent innovative foundation techniques devised for mitigating the problems posed by swelling clay beds. In a granular pile anchor, the footing is anchored to an anchor plate at the bottom of the granular pile. This makes the granular pile tension resistant and enables it to absorb the tensile force caused on the foundation by the swelling clay. An understanding of the amount of uplift resistance offered by the GPA is important in the design of granular pile-anchor foundations in field situations causing tensile forces on foundations, such as in expansive clay beds. This paper presents the results of a field-scale test program conducted to study the pullout response of GPAs embedded in expansive clay beds. Pullout load tests were conducted on GPAs of varying lengths and diameters. It was found from the field pullout load tests that granular pile anchors of larger surface area resulted in higher pullout capacity. Of the various single granular pile anchors with l/d values between 2.5 and 10, the GPA of length 1000?mm and diameter 200?mm (l/d = 5) showed the best pullout load response when tested alone, resulting in a failure uplift capacity of 14.71?kN. Increase in diameter and length of granular pile anchor increased the uplift capacity. When the length of the GPA was increased from 500 to 750 and 1000?mm, the percentage increase in the uplift load required for an upward movement of 25?mm was 33.3 and 55.5% respectively. The pullout load of the GPA when tested under group was 18?kN as against a 12?kN for the GPA when tested single.     

Exosomes in bodily fluids are a highly stable resource of disease biomarkers

Biomarkers are measurable indicators of a biological state. As our understanding of diseases meliorates, it is generally accepted that early diagnosis renders the best chance to cure a disease. In the context of proteomics, the discovery phase of identifying bonafide biomarkers and the ensuing validation phase involving large cohort of patient samples are impeded by the complexity of bodily fluid samples. High abundant proteins found in blood plasma make it difficult for the detection of low abundant proteins that may be potential biomarkers. Extracellular vesicles (EVs) have reignited interest in the field of biomarker discovery. EVs contain a tissue-type signature wherein a rich cargo of proteins and RNA are selectively packaged. In addition, as EVs are membranous structures, the luminal contents are protected from degradation by extracellular proteases and are highly stable in storage conditions. Interestingly, an appealing feature of EV-based biomarker analysis is the significant reduction in the sample complexity compared to whole bodily fluids. With these prescribed attributes, which are the rate-limiting factors of traditional biomarker analysis, there is immense potential for the use of EVs for biomarker detection in clinical settings. This review will discuss the current issues with biomarker analysis and the potential use of EVs as reservoirs of disease biomarkers.